2016-12-07The functional behaviour of haematopoietic stem cells is determined in a cell-autonomous manner by clone-specific epigenetic modifications.
2016-11-30Cadherin-based, finger-like cell–cell contacts are shown to serve as instructive structural cues that coordinate motility during collective cell migration.
2016-12-19A chemical approach for precisely mapping nucleosomes genome-wide provides novel insights into the regulation of transcription and splicing.
2016-12-19The newly identified SND pathway is an alternative route for the targeting of proteins to the endoplasmic reticulum that functions in parallel to the SRP and GET pathways.
2016-12-19OSKM-mediated reprogramming to pluripotency in vivo is facilitated by cellular senescence through the secretion of key cytokines.
2016-12-19Caenorhabditis elegans germ cell granules (P granules) are RNA and protein condensates that are associated with RNA metabolism. In C. elegans zygotes, P granules are spatially restricted to the posterior pole and, similarly to other non-membranous organelles, their formation is driven by liquid–liquid
2016-12-19The repair of DNA double-strand breaks by non-homologous end-joining (NHEJ) is efficient in non-dividing cells, but its use for site-specific transgene integration has not been shown. Suzuki et al. developed homology-independent targeted integration (HITI) for CRISPR–Cas9- and NHEJ-based gene knock-in. HITI successfully mediated site-directed
2016-12-19Recent studies have revealed that genomes contain thousands of small open reading frames that encode microproteins, but the functional significance of microproteins is unclear. D'Lima et al. now report the identification of a human microprotein, NoBody (non-annotated P-body dissociating polypeptide), that interacts with the
2016-10-05The narrow membrane necks formed during viral, exosomal and intra-endosomal budding from membranes, as well as during cytokinesis and related processes, have interiors that are contiguous with the cytosol. Severing these necks involves action from the opposite face of the membrane as occurs during the
2016-09-28Alternative polyadenylation (APA) is an RNA-processing mechanism that generates distinct 3′ termini on mRNAs and other RNA polymerase II transcripts. It is widespread across all eukaryotic species and is recognized as a major mechanism of gene regulation. APA exhibits tissue specificity and is important for
2016-11-03The recent discovery of reversible mRNA methylation has opened a new realm of post-transcriptional gene regulation in eukaryotes. The identification and functional characterization of proteins that specifically recognize RNA N6-methyladenosine (m6A) unveiled it as a modification that cells utilize to accelerate
2016-09-28Contact inhibition of locomotion (CIL) is a process whereby a cell ceases motility or changes its trajectory upon collision with another cell. CIL was initially characterized more than half a century ago and became a widely studied model system to understand how cells migrate and
2016-11-23Human pluripotent stem cells (hPSCs) provide an unparalleled opportunity to establish in vitro differentiation models that will transform our approach to the study of human development. In the case of the blood system, these models will enable investigation of the earliest stages of human