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Preview: Nature Reviews Genetics - Issue - nature.com science feeds

Nature Reviews Genetics - Issue - nature.com science feeds





 



Genetic screens: Combining CRISPR perturbations and RNA-seq

2017-01-18

Pooled genetic screens have typically been restricted to studies of fairly simplistic phenotypes (such as cell survival or reporter gene expression) in bulk cell populations, and require follow-up studies to dissect gene function in detail. Three studies published in Cell report new screening strategies



Development: Modifying sex in flies

2016-12-28

N6-methyladenosine (m6A) is the most common mRNA modification in eukaryotes, but its functions are not well understood. Now, two studies in Nature report a role for m6A in the regulation of sex determination in Drosophila melanogaster.Lence et



Genetic engineering: A genome-editing off switch

2016-12-28

The CRISPR–Cas9 system has revolutionized molecular biology and biotechnology, rapidly becoming a commonplace laboratory technique. But concerns over off-target effects still limit its use. In a study published in Cell, Pawluk et al. identify natural regulators of Cas9 in Neisseria meningitidis, which



Genetic engineering: CREATE-ing genome-wide designed mutations

2016-12-19

CRISPR–Cas genome engineering is progressively being applied for diverse high-throughput functional genomics screens. A new study reports CRISPR-enabled trackable genome engineering (CREATE) as a method for genome-scale generation of designed mutations in bacteria, with barcode-based monitoring of their phenotypic consequences.High-throughput applications of CRISPR-Cas systems



RNA: The code in the cap

2016-12-28

The discovery that reversible mRNA modifications provide a tuneable layer regulating gene expression has galvanized the field of epitranscriptomics. Now, Mauer et al. report that one of the most prevalent modified bases, N6,2′-O-dimethyladenosine (m6Am), found in



Functional genomics: Screening for lncRNA function

2017-01-03

Biological functions are known for only a small number of long non-coding RNAs (lncRNAs). A study in Science now reports a highly specific, high-throughput CRISPR-based approach to screen for functional lncRNAs.High-throughput functional screening of non-coding DNA using CRISPR–Cas9 is complicated by the fact



Regulatory activities of transposable elements: from conflicts to benefits

2016-11-21

Transposable elements (TEs) are a prolific source of tightly regulated, biochemically active non-coding elements, such as transcription factor-binding sites and non-coding RNAs. Many recent studies reinvigorate the idea that these elements are pervasively co-opted for the regulation of host genes. We argue that the inherent



Making sense of genomic islands of differentiation in light of speciation

2016-11-14

As populations diverge, genetic differences accumulate across the genome. Spurred by rapid developments in sequencing technology, genome-wide population surveys of natural populations promise insights into the evolutionary processes and the genetic basis underlying speciation. Although genomic regions of elevated differentiation are the focus of searches



Order from clutter: selective interactions at mammalian replication origins

2016-11-21

Mammalian chromosome duplication progresses in a precise order and is subject to constraints that are often relaxed in developmental disorders and malignancies. Molecular information about the regulation of DNA replication at the chromatin level is lacking because protein complexes that initiate replication seem to bind



Dissecting the genetics of complex traits using summary association statistics

2016-11-14

During the past decade, genome-wide association studies (GWAS) have been used to successfully identify tens of thousands of genetic variants associated with complex traits and diseases. These studies have produced extensive repositories of genetic variation and trait measurements across large numbers of individuals, providing tremendous



Mosaicism in health and disease — clones picking up speed

2016-12-12

Post-zygotic variation refers to genetic changes that arise in the soma of an individual and that are not usually inherited by the next generation. Although there is a paucity of research on such variation, emerging studies show that it is common: individuals are complex mosaics