Subscribe: Nature Protocols
http://www.nature.com/nprot/current_issue/rss/index.html
Preview: Nature Protocols

Nature Protocols



Nature Protocols is an interactive online resource for laboratory protocols for bench researchers. The core content is high quality, peer-reviewed protocols that are presented in a 'recipe' style, providing step-by-step descriptions of procedures that use



 



Improving your four-dimensional image: traveling through a decade of light-sheet-based fluorescence microscopy research

2017-05-04

This Perspective discusses the development and applications of light-sheet-based fluorescence microscopy, a method with considerable advantages over confocal fluorescence microscopy.



Using hyperLOPIT to perform high-resolution mapping of the spatial proteome

2017-05-04

This protocol describes a substantially redeveloped approach for the localization of organelle proteins by isotope tagging (LOPIT) to enable subcellular localization of thousands of proteins per experiment (hyperLOPIT).



Comprehensive analysis of mouse retinal mononuclear phagocytes

2017-05-04

This protocol describes how to characterize mouse retinal mononuclear phagocytes in vivo and in situ using scanning laser ophthalmoscopy of microglia, quantitative analysis of Iba1-stained retinal sections, CD11b-based retinal flow cytometry, and qRT–PCR analysis of key microglia markers.



Single-cell template strand sequencing by Strand-seq enables the characterization of individual homologs

2017-05-11

Strand-seq is a single-cell template strand sequencing technology that generates directional genomic libraries, which allows the characterization of individual homologs.



Differentiation of cardiomyocytes and generation of human engineered heart tissue

2017-05-11

This protocol describes how to generate defined embryoid bodies and subsequent standardized beating engineered heart tissue from human iPSCs using small molecules.



Super-resolution microscopy with DNA-PAINT

2017-05-18

In DNA-PAINT, transient binding of dye-labeled oligonucleotides to their target strands creates the ‘blinking’ required for stochastic nanoscopy. This protocol describes how to apply DNA-PAINT, from sample preparation to data processing.



Simultaneous quantification of N- and O-glycans using a solid-phase method

2017-05-18

The comprehensive study of protein glycosylation has been complicated by the complex structural diversity of glycans. In this protocol, Yang et al. describe a solid-phase method for the sequential analysis of N-linked and O-linked glycans.



FISH-Flow, a protocol for the concurrent detection of mRNA and protein in single cells using fluorescence in situ hybridization and flow cytometry

2017-05-18

FISH-Flow is a flow-cytometry-based protocol enabling simultaneous mRNA and protein measurements in single nonadherent mammalian cells. The authors provide both a manual and a semiautomated, single-tube version of the protocol.



Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples

2017-05-24

This multiplex PCR enrichment protocol enables sequencing of Zika and other viral genomes of low abundance from clinical samples using the Illumina platform, or the portable MinION sequencer, facilitating direct application in field situations.



Solid-phase synthesis, cyclization, and site-specific functionalization of aziridine-containing tetrapeptides

2017-05-24

Aziridine-containing cyclic tetrapeptides possess great potential for covalent protein labeling and can be valuable in screening libraries. Chung et al. describe the synthesis, cyclization, and site-specific modification of these scaffolds.