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Nature Protocols is an interactive online resource for all laboratory protocols relevant to biological and biomedical research. Nature Protocols includes a free-to-access community site, 'Protocols Network', containing protocols that are posted directly o


Immunofluorescent antibody staining of intact Drosophila larvae


Standard methods for antibody staining of Drosophila larvae have worked poorly or involved dissection. The Doe laboratory describes a reliable protocol for immunofluorescent antibody staining of intact Drosophila larvae that works well for all larval stages.

Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using human pluripotent stem cells


Different concentrations of activin A and BMP4 are used to polarize stem cells into mesodermal subtypes that reflect mid-primitive-streak cardiogenic mesoderm and posterior-primitive-streak hemogenic mesoderm.

Automated identification of mouse visual areas with intrinsic signal imaging


This protocol describes how to produce retinotopic maps of mouse visual cortex using intrinsic signal optical imaging and a segmentation algorithm.

Single-cell barcoding and sequencing using droplet microfluidics


Zilionis et al. describe a protocol for the inDrops platform, which is a high-throughput droplet microfluidics system that allows for single-cell barcoding of over 15,000 cells in 1 h.

Merging allylic C–H bond activation and C–C bond cleavage en route to the formation of a quaternary carbon stereocenter in acyclic systems


This protocol describes the diastereoselective approach for the synthesis of complex, acyclic molecular architectures possessing two stereogenic centers in a 1,4 relationship based on a zirconium-promenade reaction using the Negishi reagent.

Efficient, footprint-free human iPSC genome editing by consolidation of Cas9/CRISPR and piggyBac technologies


This protocol uses a doxycycline-inducible Cas9 transgene carried on a piggyBac transposon for robust, highly efficient and scarless genome editing of human iPSCs, enabling a parental line to be engineered to harbor many separate mutations.