2017-01-05Single-particle cryo-electron microscopy has enabled the structures of large proteins to be elucidated. This Perspective discusses technological improvements in this technique, focusing particularly on the past decade and likely future developments.
2017-01-05In this Perspective, Elaine Mardis reviews a decade of DNA sequencing technology, from the introduction of Next-Generation Sequencing to single-molecule sequencing, including future applications that promise to further biological and biomedical research.
2017-01-05This protocol extension describes the fabrication of optofluidic neural probes and implantation for advanced in vivo pharmacology and optogenetics in freely moving rodents.
2017-01-05Moreno-Romero et al. describe how to apply the INTACT method to purify endosperm nuclei from Arabidopsis. Purified nuclei can be used for ChIP-seq analysis or bisulfite sequencing.
2017-01-12The metabolome or lipidome of different phenotypes can be compared using this protocol. A complete workflow for direct-infusion mass spectrometry using nanoelectrospray ionization and spectral stitching with the Galaxy platform is described.
2017-01-12ClusPro is a web server that performs rigid-body docking of two proteins by sampling billions of conformations. Low-energy docked structures are clustered, and centers of the largest clusters are used as likely models of the complex.
2017-01-12This protocol from Wang et al. describes a pulse–chase method to investigate autophagic protein degradation through click labeling of long-lived proteins. This is a safer alternative to similar classic methods that use radioactive labeling.
2017-01-12Friedrich et al. describe their toolkit for transposon-based insertional mutagenesis in mice for discovering cancer genes. Genome-wide transposon insertion sites are identified, mapped and quantified using QiSeq.
2017-01-19CORRECT is a method comprising two variants, re-Guide and re-Cas, that enables efficient and scarless CRISPR/Cas9-based genome editing in hPSCs by introducing blocking mutations that hamper the re-editing of modified loci by Cas9.
2017-01-19This protocol provides details for dispersion and colloidal characterization of suspended engineered nanomaterials, and computational fate and transport modeling to accurately calculate dose metrics for in vitro cellular nanotoxicology experiments.
2017-01-19Detailed guides for the husbandry of Xenopus laevis and protocols for making reproducible spinal cord injury models and subsequent study of neural stem/progenitor cells during regeneration are described.