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Nature Protocols

Nature Protocols is an interactive online resource for laboratory protocols for bench researchers. The core content is high quality, peer-reviewed protocols that are presented in a 'recipe' style, providing step-by-step descriptions of procedures that use


Quantitative proteomics: challenges and opportunities in basic and applied research


In their Perspective, Schubert et al. discuss developments and challenges in mass-spectrometry-based proteomics technology in the past decade and explore its role in molecular systems biology, clinical research and personalized medicine.

Chemically induced mouse models of acute and chronic intestinal inflammation


This protocol update describes how to generate mouse models of inflammatory bowel diseases and methods for analyzing disease progression.

Integrating macromolecular X-ray diffraction data with the graphical user interface iMosflm


The MOSFLM software is widely used for X-ray diffraction data integration. The graphical user interface version, iMosflm, now makes this powerful software program accessible to inexperienced users.

mRNA quantification using single-molecule FISH in Drosophila embryos


Single-molecule fluorescence in situ hybridization (smFISH) enables mRNA quantification while preserving spatial information. Trcek et al. describe an smFISH procedure for Drosophila embryos, using wide-field, confocal and structured illumination miscroscopy.

Preparation of biomimetic hierarchically helical fiber actuators from carbon nanotubes


Deng et al. describe a method for preparing mechanically responsive hierarchically arranged helical fiber (HHF) actuators based on aligned carbon nanotubes. These HHF actuators are responsive to external stimuli in a rapid and reversible manner.

Biological and chemical strategies for exploring inter- and intra-kingdom communication mediated via bacterial volatile signals


Bacterial interactions with other species can occur via small, volatile metabolites. In this protocol, volatile metabolites generated by placing two species in a two-Petri-plate system are analyzed by GC–MS after solid-phase microextraction.

Assessment of social transmission of threats in humans using observational fear conditioning


This protocol describes how to assess indirectly acquired threat responses in humans. The reaction to the emotional expressions of others is determined by measuring skin conductance responses.

A human intestinal M-cell-like model for investigating particle, antigen and microorganism translocation


This protocol describes coculture of Caco-2 cells with Raji cells on inverted inserts, resulting in conversion of Caco-2 cells to a microfold (M)-cell-like model for the study of particle, antigen and microorganism translocation in vitro.

Cancer imaging using surface-enhanced resonance Raman scattering nanoparticles


This protocol describes how to make SERRS nanoparticles and presents some procedures for using them, including intraoperative, in vivo Raman imaging of cancer in mice.

Assessing recent and remote associative olfactory memory in rats using the social transmission of food preference paradigm


This protocol describes how to use the paradigm for social transmission of food preference in rats to assess recent and remote associative olfactory memory and underlying processes, including encoding, consolidation, retrieval and forgetting.

Interfacing 3D magnetic twisting cytometry with confocal fluorescence microscopy to image force responses in living cells


Mechanical forces can trigger a variety of biological responses in cells and tissues. This protocol describes how to combine 3D magnetic twisting cytometry with confocal fluorescence microscopy to study these force responses in greater detail.