2017-03-30This protocol describes how to set up and use an in vitro model of the human microcirculation with the capability to recapitulate discrete steps of early metastatic seeding, including tumor cell arrest and transendothelial migration.
2017-03-30This protocol enables the study of protein complexes by trapping them in virus-like particles and thereby preserving cellular integrity. The Virotrap protocol is complemented by SFINX, an online data analysis tool for interactomics studies.
2017-03-30Li et al. provide a protocol for long-read ChIA-PET, a technique for mapping chromatin interactions. The longer paired-end tags, which are generated by tagmentation, provide sufficient coverage to determine haplotype-specific chromatin interactions at single-nucleotide resolution.
2017-04-06Four different techniques for preparing and acquiring super-resolution CLEM data sets on aldehyde-fixed specimens are provided: Tokuyasu cryosectioning; whole-cell mount; cell unroofing and platinum replication; and resin embedding and sectioning.
2017-04-06By combining minimal inhibitory concentration testing with GC–MS-based pathway analysis, it is possible to identify which enzymes in the ergosterol biosynthesis pathway are being targeted by potential new antifungal substances.
2017-04-06This protocol describes how to produce molecularly imprinted polymers, synthetic receptors that can be produced with antibody-like binding properties. They are easier and cheaper to produce than antibodies and lectins.
2017-04-13This protocol describes the preparation of calibration bead slides, their use and additional strategies to reduce artifacts of structured illumination microscopy that will allow researchers to exploit the technique's full potential for biological applications.
2017-04-13This protocol describes how to prepare samples for labeling nuclei of cultured mammalian cells for 3D structured illumination microscopy of nuclear structures. Image acquisition, registration and downstream image analysis are also described.
2017-04-20Expandable and multipotent induced cardiac progenitor cells (iCPCs) are generated from mouse adult fibroblasts using forced expression of Mesp1, Tbx5, Gata4, Nkx2.5 and Baf60c and activation of the Wnt and JAK/STAT signaling pathway. Furthermore, embryonic potency of iCPCs is tested ex vivo.
2017-04-20Bioluminescence and radioluminescence are both dim phenomena that require a highly sensitive microscope for imaging at the cellular level. This protocol describes how to construct a modular low-light microscope for imaging these events.