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Nature Protocols



Nature Protocols is an interactive online resource for laboratory protocols for bench researchers. The core content is high quality, peer-reviewed protocols that are presented in a 'recipe' style, providing step-by-step descriptions of procedures that use



 



The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals

2017-08-03

Here the authors provide an extension of their original FLOTAC protocol, describing the Mini-FLOTAC technique, optimized to perform diagnosis of helminth and protozoan infections in humans and animals where centrifugation may not be practical.



Reconstitution of mouse oogenesis in a dish from pluripotent stem cells

2017-08-10

This protocol extension describes how to obtain functionally mature oocytes from embryonic or induced pluripotent stem cells. These oocytes can be used to produce live mouse offspring.



The cubicon method for concentrating membrane proteins in the cubic mesophase

2017-08-03

The lipid cubic phase (in meso) method is used for generating crystals and X-ray structures of integral membrane proteins. This protocol describes the cubicon method for concentrating membrane proteins in the cubic mesophase.



Mechanical isolation, and measurement of force and myoplasmic free [Ca2+] in fully intact single skeletal muscle fibers

2017-08-03

Cheng & Westerblad describe the mechanical dissection of living single fibers from the mouse flexor digitorum brevis muscle. Isolated intact fibers are subsequently used for force and myoplasmic free [Ca2+] measurements.



Use of fluorescence-detected sedimentation velocity to study high-affinity protein interactions

2017-08-03

This protocol describes how to use sedimentation velocity analytical ultracentrifugation in combination with fluorescence optical detection for the analysis of mass, shape, size distribution, and binding constants of interacting proteins.



Electron microscopy using the genetically encoded APEX2 tag in cultured mammalian cells

2017-08-10

This protocol describes procedures for using the genetic tag APEX2 to generate contrast for electron microscopy in cultured cells.



Development of fertile mouse oocytes from mitotic germ cells in vitro

2017-08-10

This protocol describes how to obtain functionally mature oocytes from primordial germ cells from fetal mouse ovaries in vitro. These oocytes can be used to produce live mouse offspring.



Intracellular directed evolution of proteins from combinatorial libraries based on conditional phage replication

2017-08-10

This protocol describes a phagemid-based intracellular evolution approach to generate and select for proteins with improved biological characteristics.



Meganuclease-assisted generation of stable transgenics in the sea anemone Nematostella vectensis

2017-08-17

The Technau lab provides their protocol for the generation of stably transgenic sea anemones. An expression vector is digested with the meganuclease I-SceI and then microinjected into embryos, where I-SceI mediates stable integration into the genome.



Evaluation of telomere length in human cardiac tissues using cardiac quantitative FISH

2017-08-17

This protocol describes how to measure telomere length in archival human cardiac tissues using cardiac quantitative fluorescent in situ hybridization (CQ-FISH) in a cell-type-specific manner.



Single-cell mechanogenetics using monovalent magnetoplasmonic nanoparticles

2017-08-17

This protocol describes how to exert precise spatial and mechanical control over genetically encoded cell-surface receptors in live cells using magnetoplasmonic nanoparticles.



Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions

2017-08-17

This protocol differentiates hPSCs into self-renewing epicardial cells by appropriate differentiation-stage-specific application of Gsk3 inhibitor, Wnt inhibitor, and then Gsk3 inhibitor again in a completely defined, xeno-free system.