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Nature Protocols is an interactive online resource for all laboratory protocols relevant to biological and biomedical research. Nature Protocols includes a free-to-access community site, 'Protocols Network', containing protocols that are posted directly o



 



The past, present and future of microbiome analyses

2016-09-29

This Perspective from Janet Jansson and colleagues reviews the development of microbiome analysis technologies over the last decade, and comments on the future potential of this fast-moving field.



Resolving macromolecular structures from electron cryo-tomography data using subtomogram averaging in RELION

2016-09-29

Electron cryo-tomography produces 3D pictures of unique objects such as viruses, organelles or cells. This is a protocol for macromolecular structure determination from tomographic data using subtomogram averaging in the RELION software.



A simple approach for measuring FRET in fluorescent biosensors using two-photon microscopy

2016-09-29

This protocol describes a simple pipeline for imaging FRET biosensor probes with two-photon laser scanning microscopy (TPLSM). Validated FRET standards are described that can be used with any TPLSM setup to ensure sensitive detection of FRET ratios.



Separation and parallel sequencing of the genomes and transcriptomes of single cells using G&T-seq

2016-09-29

G&T-seq enables sequencing of DNA and mRNA from the same single cell, allowing the effects of genomic variation on transcription to be studied. It is compatible with any WGA method and so can be tailored to specific applications.



Mapping 3D genome architecture through in situ DNase Hi-C

2016-09-29

Ramani et al. describe a protocol for in situ DNase Hi-C as an alternative to traditional Hi-C methods that use restriction enzymes. The use of DNase I for chromatin digestion circumvents the resolution limit imposed when relying on genomic restriction sites.