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Nature Protocols



Nature Protocols is an interactive online resource for laboratory protocols for bench researchers. The core content is high quality, peer-reviewed protocols that are presented in a 'recipe' style, providing step-by-step descriptions of procedures that use



 



Mapping the small RNA interactome in bacteria using RIL-seq

2017-12-07

This protocol describes an experimental–computational methodology for mapping the small RNA interactome in bacteria.



Expansion of patient-derived circulating tumor cells from liquid biopsies using a CTC microfluidic culture device

2017-12-07

This protocol describes a microfluidics approach for culturing liquid-biopsy-derived circulating tumor cell clusters to predict a patient's response toward various therapeutic strategies.



Measuring mutation accumulation in single human adult stem cells by whole-genome sequencing of organoid cultures

2017-12-07

This protocol describes a method for cataloging genome-wide mutations that accumulated during life or culture in single adult stem cells of different human tissues, by combining whole-genome sequencing with organoid-culture technologies.



Assembly of phospholipid nanodiscs of controlled size for structural studies of membrane proteins by NMR

2017-12-07

The applications of solution-state NMR of membrane proteins are often limited by difficulty in finding a suitable membrane mimetic of tailored size that shows native-like membrane properties and provides long-term stability. This protocol describes how to assemble phospholipid nanodiscs and incorporate membrane proteins for NMR-structural studies.



Transient expression of human antibodies in mammalian cells

2017-12-14

Mammalian cells are powerful expression systems for producing glycosylated recombinant antibody preparations with minimal endotoxin contamination. This protocol describes procedures for antibody design, expression, purification and characterization.



Use of TAI-FISH to visualize neural ensembles activated by multiple stimuli

2017-12-14

This protocol describes a dual mRNA and protein labeling strategy that allows identification of activated neuronal assemblies in response to two temporally separated stimuli in mouse brain sections.



3D molecular cartography using LC–MS facilitated by Optimus and 'ili software

2017-12-21

3D molecular cartography is used for mapping of metabolites in our environment. This protocol describes the procedures for sample collection and processing, mass spectrometry analysis, and data processing and visualization.



Live-cell measurements of kinase activity in single cells using translocation reporters

2017-12-21

This protocol describes how to design and use fluorescent translocation reporters for live measurements of kinase activity in single cells.



Single-cell microscopy of suspension cultures using a microfluidics-assisted cell screening platform

2017-12-21

This protocol describes a microfluidics platform, termed 'MACS', for single-cell microscopy of suspension cells. MACS temporarily immobilizes the cells, allowing for high-throughput and high-resolution imaging, cell sorting, and single-molecule detection.



Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors

2017-12-21

This protocol describes Easi-CRISPR, a method for creating knock-in, conditional knockout, and knockdown mouse models by CRISPR/Cas9-based genome engineering using long single-stranded DNA donor templates.