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Nature Protocols is an interactive online resource for all laboratory protocols relevant to biological and biomedical research. Nature Protocols includes a free-to-access community site, 'Protocols Network', containing protocols that are posted directly o



 



Immunofluorescent antibody staining of intact Drosophila larvae

2016-12-01

Standard methods for antibody staining of Drosophila larvae have worked poorly or involved dissection. The Doe laboratory describes a reliable protocol for immunofluorescent antibody staining of intact Drosophila larvae that works well for all larval stages.



Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using human pluripotent stem cells

2016-12-01

Different concentrations of activin A and BMP4 are used to polarize stem cells into mesodermal subtypes that reflect mid-primitive-streak cardiogenic mesoderm and posterior-primitive-streak hemogenic mesoderm.



Automated identification of mouse visual areas with intrinsic signal imaging

2016-12-01

This protocol describes how to produce retinotopic maps of mouse visual cortex using intrinsic signal optical imaging and a segmentation algorithm.



Single-cell barcoding and sequencing using droplet microfluidics

2016-12-08

Zilionis et al. describe a protocol for the inDrops platform, which is a high-throughput droplet microfluidics system that allows for single-cell barcoding of over 15,000 cells in 1 h.



Merging allylic C–H bond activation and C–C bond cleavage en route to the formation of a quaternary carbon stereocenter in acyclic systems

2016-12-08

This protocol describes the diastereoselective approach for the synthesis of complex, acyclic molecular architectures possessing two stereogenic centers in a 1,4 relationship based on a zirconium-promenade reaction using the Negishi reagent.



Efficient, footprint-free human iPSC genome editing by consolidation of Cas9/CRISPR and piggyBac technologies

2016-12-08

This protocol uses a doxycycline-inducible Cas9 transgene carried on a piggyBac transposon for robust, highly efficient and scarless genome editing of human iPSCs, enabling a parental line to be engineered to harbor many separate mutations.