2016-12-29Neurons that are activated in response to a particular stimulus or behavior can be labeled or manipulated with a new method.
2016-12-29A multicolor approach specifically labels multiple targets in electron microscopy images.
2016-12-29A rapid mix-and-inject serial femtosecond crystallography approach enables structure determination of ligand-binding intermediates.
2016-12-29A synthetic hydrogel matrix gives researchers greater control over intestinal organoid culture.
2016-12-29The development of temperature-inducible molecular bioswitches allows on-demand activation of synthetic genetic circuits in microbes.
2016-12-29This Review describes the latest methods for profiling common epitranscriptomic marks, their scale, resolution and ability to quantify. It also discusses remaining challenges.
2016-12-29The ability to measure the metabolome on a global scale lags behind other omics techniques.
2016-12-29The changing face of super-resolution imaging
2016-12-29Having revolutionized DNA editing, CRISPR turns to RNA.
2016-12-29Single-cell sequencing is poised to elucidate how cells contribute to tissue function.
2016-12-29Cryo-electron tomography may facilitate in situ structural biology on a proteomic scale.
2016-12-29Higher volumetric imaging rates shed light on the dynamics in neuronal networks.
2016-12-29New approaches will expose microbial dependencies and environmental interactions.
2016-12-29Ex vivo organoid culture could revolutionize biology, but variability must be understood.
2016-12-29Sequencing technology drives microbiology and gives researchers new reasons to draw on classic techniques.
2016-11-21Three-dimensional localization microscopy can yield important biological insights. A photometric approach is described that allows users to gain 3D information from existing 2D images and to improve axial resolution obtained with existing biplane setups.
2016-11-07A new method, R-scape, tests whether observed sequence covariation supports a conserved secondary structure in RNA. The program finds no evidence for previously proposed conserved secondary structures in several lncRNAs.
2016-11-21Membrane proteins can be stabilized in a native-like setting using lipid-bilayer-based nanodiscs encircled by a membrane scaffold protein. Covalently circularized nanodiscs now offer enhanced stability and control over nanodisc diameter size, improving the quality of structural data.
2016-11-21An extremely bright, truly monomeric RFP, mScarlet, is described that outperforms existing RFPs in diverse labeling applications, especially in FRET with ratiometric imaging.
2016-11-14The authors present a computational framework for false-discovery-rate-controlled metabolite annotation from high-resolution imaging mass spectrometry data.
2016-11-28InWeb_InBioMap (InWeb_IM for short) is a scored, integrated human protein–protein interaction network resource aggregated from public, experimentally determined protein–protein interactions. The resource enables functional interpretation of large-scale genomics data.
2016-11-28The novoBreak software provides sensitive, accurate, and comprehensive detection of structural variation in somatic next-generation sequencing data.
2016-11-21TACO generates a consensus transcriptome with improved accuracy from multisample RNA-seq data.
2016-11-07An all-atom protein force field, CHARMM36m, offers improved accuracy for simulating intrinsically disordered peptides and proteins.
2016-11-07DMS-MaPseq enables genome-wide and target-specific RNA secondary structure probing of even rare or heterogeneously structured RNAs in vivo and was used to study structure involved in translation regulation as well as nascent transcripts.
2016-11-07BUM-HMM is a statistically robust modeling pipeline for interpreting high-throughput RNA structure probing data, including that from transcriptome-wide experiments.
2016-11-14An instrumental setup for atmospheric pressure MALDI-based mass spectrometry imaging with improved lateral resolution enables subcellular-level details to be resolved.