Subscribe: Nature Methods - Issue - nature.com science feeds
http://www.nature.com/nmeth/current_issue/rss
Added By: Feedage Forager Feedage Grade B rated
Language: English
Tags:
analysis  biology  cell rna  cell  heterogeneity  human  protein  proteins  rna  seq  sequencing  single cell  single  synthetic human 
Rate this Feed
Rate this feedRate this feedRate this feedRate this feedRate this feed
Rate this feed 1 starRate this feed 2 starRate this feed 3 starRate this feed 4 starRate this feed 5 star

Comments (0)

Feed Details and Statistics Feed Statistics
Preview: Nature Methods - Issue - nature.com science feeds

Nature Methods - Issue - nature.com science feeds



Nature Methods is a science methodology journal publishing laboratory techniques and methods papers in the life sciences and areas of chemistry relevant to the life sciences.



 



Responsible referencing

2017-02-28

Careful construction of reference lists is the responsibility of every scientist.



The Author File: Bart Deplancke

2017-02-28

An engineered approach to study gene regulation, and why home is where the heart is.



Points of Significance: Interpreting P values

2017-02-28

A P value measures a sample's compatibility with a hypothesis, not the truth of the hypothesis.


















Microscopy: Taking nanoscopy to the limit

2017-02-28

MINFLUX nanoscopy can obtain 1-nm localization precision with orders of magnitude fewer emitted photons.



Stem cells: Cerebral organoids enter the fold

2017-02-28

Two groups use cerebral organoids to model brain development and disease.



Genomics: Another player for RNA-guided RNA cleavage

2017-02-28

Cas13b relies on activator and repressor proteins to regulate RNA cleavage.



Cell biology: Precision switches for protein control

2017-02-28

Light- or drug-dependent switches enable precise control over signaling pathways in living cells.



























Structural biology: Protein holography

2017-02-28

Low-energy holography enables imaging single proteins and protein complexes.






Cell biology: tracking a cell's cycle

2017-02-28

The tools that clock a cell's everyday affairs reveal plenty that's out of the ordinary.



Genetic screening enters the single-cell era

2017-02-28

Four studies overcome the limitations of pooled and arrayed genetic screens by integrating single-cell transcriptomics.



Stratifying tissue heterogeneity with scalable single-cell assays

2017-02-28

Three methods dramatically improve the scale of single-cell genomic sequencing and structural assays, allowing for the hierarchical partitioning of cell populations within a tissue.



Synthetic human proteomes for accelerating protein research

2017-02-28

The generation of synthetic human proteomes and future derived tools will expand knowledge in protein biology.



A guide to designing germline-dependent epigenetic inheritance experiments in mammals

2017-02-28

This Review discusses key aspects of experimental design to evaluate intergenerational and transgenerational epigenetic inheritance. It describes advantages of patrilineal and matrilineal design, and it offers advice for selecting animals for breeding and for determining the weaning scheme and group size.



A large-scale targeted proteomics assay resource based on an in vitro human proteome

2016-12-26

A large-scale resource, iMPAQT, provides multiple reaction monitoring (MRM)–mass spectrometry assays for targeted quantitative analysis of mTRAQ-labeled human proteins.



Building ProteomeTools based on a complete synthetic human proteome

2017-01-30

The ProteomeTools project provides the proteomics community with a physical synthetic tryptic peptide resource and a digital LC-MS/MS data resource covering all human proteins.



Massively multiplex single-cell Hi-C

2017-01-30

Single-cell combinatorial indexed Hi-C (sciHi-C) is a streamlined protocol for generating thousands of high-quality single-cell chromosome conformation data sets that resemble bulk Hi-C data in aggregate.



Effective detection of variation in single-cell transcriptomes using MATQ-seq

2017-01-16

MATQ-seq is a highly sensitive single-cell RNA-seq protocol that enables the detection of true subtle biological variations among single cells as well as the capture of nonpolyadenylated RNA.



Optogenetic inhibition of behavior with anion channelrhodopsins

2017-01-23

Anion channelrhodopsins are light-sensitive chloride channels that can be used as optogenetic inhibitors. Mohammad et al. report their application in Drosophila, showing that various behaviors can be inhibited in a light-dependent manner.



Characterizing cell subsets using marker enrichment modeling

2017-01-30

Marker enrichment modeling (MEM) provides an objective metric for characterizing cell populations from high-content single-cell analysis. The MEM score outperforms standard metrics and provides a machine-readeable label for cell subsets.



Post-translational selective intracellular silencing of acetylated proteins with de novo selected intrabodies

2017-01-16

PISA, generates intrabodies that selectively bind and interfere with the intracellular function of an acetylated version of a target protein.



High-fidelity mass analysis unveils heterogeneity in intact ribosomal particles

2017-01-23

Instrumental modifications enable native mass spectrometry analysis with unprecedented mass resolution, especially at high mass-to-charge ratios, as illustrated through the analysis of intact ribosome particles.



One-step generation of conditional and reversible gene knockouts

2017-01-30

The combination of knocking one allele out with CRISPR-mediated NHEJ and targeting the other with a conditionally inactivating cassette allows rapid generation of conditional alleles.



cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

2017-02-06

A software tool, cryoSPARC, addresses the speed bottleneck in cryo-EM image processing, enabling automated macromolecular structure determination in hours on a desktop computer without requiring a starting model.



Pooled CRISPR screening with single-cell transcriptome readout

2017-01-18

CROP-seq enables pooled CRISPR screens for complex transcriptome signatures by making gRNA expression detectable in single-cell RNA sequencing.



Sequencing thousands of single-cell genomes with combinatorial indexing

2017-01-30

Single-cell combinatorial indexed sequencing (SCI-seq) resolves genomic heterogeneity by generating thousands of low-pass single-cell libraries at once for somatic copy number variant detection.



Single-cell mRNA quantification and differential analysis with Census

2017-01-23

The Census tool converts single-cell RNA-seq relative read counts to relative transcript counts for more accurate differential gene expression and analysis in the absence of spike-ins or molecular barcodes.



SMiLE-seq identifies binding motifs of single and dimeric transcription factors

2017-01-16

The Microfluidic-based Ligand Enrichment (SMiLE) sequencing strategy probes DNA binding of single and heterodimeric transcription factors over a wide affinity range.