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Preview: Nature Methods - Issue - nature.com science feeds

Nature Methods - Issue - nature.com science feeds



Nature Methods is a science methodology journal publishing laboratory techniques and methods papers in the life sciences and areas of chemistry relevant to the life sciences.



 












Neuroscience: Manipulating neurons in an activity-dependent manner

2016-12-29

Neurons that are activated in response to a particular stimulus or behavior can be labeled or manipulated with a new method.



Microscopy: Colorful electron microscopy

2016-12-29

A multicolor approach specifically labels multiple targets in electron microscopy images.



Structural biology: Blend-and-shoot crystallography

2016-12-29

A rapid mix-and-inject serial femtosecond crystallography approach enables structure determination of ligand-binding intermediates.



Stem cells: Move over, Matrigel

2016-12-29

A synthetic hydrogel matrix gives researchers greater control over intestinal organoid culture.



























Synthetic biology: Thermal therapeutics

2016-12-29

The development of temperature-inducible molecular bioswitches allows on-demand activation of synthetic genetic circuits in microbes.









Epitranscriptome sequencing technologies: decoding RNA modifications

2016-12-29

This Review describes the latest methods for profiling common epitranscriptomic marks, their scale, resolution and ability to quantify. It also discusses remaining challenges.



Global metabolomics

2016-12-29

The ability to measure the metabolome on a global scale lags behind other omics techniques.



Expansion microscopy

2016-12-29

The changing face of super-resolution imaging



CRISPR targets RNA

2016-12-29

Having revolutionized DNA editing, CRISPR turns to RNA.



How single cells do it

2016-12-29

Single-cell sequencing is poised to elucidate how cells contribute to tissue function.



Cryo-electron tomography

2016-12-29

Cryo-electron tomography may facilitate in situ structural biology on a proteomic scale.



Faster brain imaging

2016-12-29

Higher volumetric imaging rates shed light on the dynamics in neuronal networks.



Capturing microbial interactions

2016-12-29

New approaches will expose microbial dependencies and environmental interactions.



Organoid culture

2016-12-29

Ex vivo organoid culture could revolutionize biology, but variability must be understood.



Microbiology: the return of culture

2016-12-29

Sequencing technology drives microbiology and gives researchers new reasons to draw on classic techniques.



Photometry unlocks 3D information from 2D localization microscopy data

2016-11-21

Three-dimensional localization microscopy can yield important biological insights. A photometric approach is described that allows users to gain 3D information from existing 2D images and to improve axial resolution obtained with existing biplane setups.



A statistical test for conserved RNA structure shows lack of evidence for structure in lncRNAs

2016-11-07

A new method, R-scape, tests whether observed sequence covariation supports a conserved secondary structure in RNA. The program finds no evidence for previously proposed conserved secondary structures in several lncRNAs.



Covalently circularized nanodiscs for studying membrane proteins and viral entry

2016-11-21

Membrane proteins can be stabilized in a native-like setting using lipid-bilayer-based nanodiscs encircled by a membrane scaffold protein. Covalently circularized nanodiscs now offer enhanced stability and control over nanodisc diameter size, improving the quality of structural data.



mScarlet: a bright monomeric red fluorescent protein for cellular imaging

2016-11-21

An extremely bright, truly monomeric RFP, mScarlet, is described that outperforms existing RFPs in diverse labeling applications, especially in FRET with ratiometric imaging.



FDR-controlled metabolite annotation for high-resolution imaging mass spectrometry

2016-11-14

The authors present a computational framework for false-discovery-rate-controlled metabolite annotation from high-resolution imaging mass spectrometry data.



A scored human protein–protein interaction network to catalyze genomic interpretation

2016-11-28

InWeb_InBioMap (InWeb_IM for short) is a scored, integrated human protein–protein interaction network resource aggregated from public, experimentally determined protein–protein interactions. The resource enables functional interpretation of large-scale genomics data.



novoBreak: local assembly for breakpoint detection in cancer genomes

2016-11-28

The novoBreak software provides sensitive, accurate, and comprehensive detection of structural variation in somatic next-generation sequencing data.



TACO produces robust multisample transcriptome assemblies from RNA-seq

2016-11-21

TACO generates a consensus transcriptome with improved accuracy from multisample RNA-seq data.



CHARMM36m: an improved force field for folded and intrinsically disordered proteins

2016-11-07

An all-atom protein force field, CHARMM36m, offers improved accuracy for simulating intrinsically disordered peptides and proteins.



DMS-MaPseq for genome-wide or targeted RNA structure probing in vivo

2016-11-07

DMS-MaPseq enables genome-wide and target-specific RNA secondary structure probing of even rare or heterogeneously structured RNAs in vivo and was used to study structure involved in translation regulation as well as nascent transcripts.



Robust statistical modeling improves sensitivity of high-throughput RNA structure probing experiments

2016-11-07

BUM-HMM is a statistically robust modeling pipeline for interpreting high-throughput RNA structure probing data, including that from transcriptome-wide experiments.



Atmospheric pressure MALDI mass spectrometry imaging of tissues and cells at 1.4-μm lateral resolution

2016-11-14

An instrumental setup for atmospheric pressure MALDI-based mass spectrometry imaging with improved lateral resolution enables subcellular-level details to be resolved.