2017-02-28Careful construction of reference lists is the responsibility of every scientist.
2017-02-28An engineered approach to study gene regulation, and why home is where the heart is.
2017-02-28A P value measures a sample's compatibility with a hypothesis, not the truth of the hypothesis.
2017-02-28MINFLUX nanoscopy can obtain 1-nm localization precision with orders of magnitude fewer emitted photons.
2017-02-28Two groups use cerebral organoids to model brain development and disease.
2017-02-28Cas13b relies on activator and repressor proteins to regulate RNA cleavage.
2017-02-28Light- or drug-dependent switches enable precise control over signaling pathways in living cells.
2017-02-28Low-energy holography enables imaging single proteins and protein complexes.
2017-02-28The tools that clock a cell's everyday affairs reveal plenty that's out of the ordinary.
2017-02-28Four studies overcome the limitations of pooled and arrayed genetic screens by integrating single-cell transcriptomics.
2017-02-28Three methods dramatically improve the scale of single-cell genomic sequencing and structural assays, allowing for the hierarchical partitioning of cell populations within a tissue.
2017-02-28The generation of synthetic human proteomes and future derived tools will expand knowledge in protein biology.
2017-02-28This Review discusses key aspects of experimental design to evaluate intergenerational and transgenerational epigenetic inheritance. It describes advantages of patrilineal and matrilineal design, and it offers advice for selecting animals for breeding and for determining the weaning scheme and group size.
2016-12-26A large-scale resource, iMPAQT, provides multiple reaction monitoring (MRM)–mass spectrometry assays for targeted quantitative analysis of mTRAQ-labeled human proteins.
2017-01-30The ProteomeTools project provides the proteomics community with a physical synthetic tryptic peptide resource and a digital LC-MS/MS data resource covering all human proteins.
2017-01-30Single-cell combinatorial indexed Hi-C (sciHi-C) is a streamlined protocol for generating thousands of high-quality single-cell chromosome conformation data sets that resemble bulk Hi-C data in aggregate.
2017-01-16MATQ-seq is a highly sensitive single-cell RNA-seq protocol that enables the detection of true subtle biological variations among single cells as well as the capture of nonpolyadenylated RNA.
2017-01-23Anion channelrhodopsins are light-sensitive chloride channels that can be used as optogenetic inhibitors. Mohammad et al. report their application in Drosophila, showing that various behaviors can be inhibited in a light-dependent manner.
2017-01-30Marker enrichment modeling (MEM) provides an objective metric for characterizing cell populations from high-content single-cell analysis. The MEM score outperforms standard metrics and provides a machine-readeable label for cell subsets.
2017-01-16PISA, generates intrabodies that selectively bind and interfere with the intracellular function of an acetylated version of a target protein.
2017-01-23Instrumental modifications enable native mass spectrometry analysis with unprecedented mass resolution, especially at high mass-to-charge ratios, as illustrated through the analysis of intact ribosome particles.
2017-01-30The combination of knocking one allele out with CRISPR-mediated NHEJ and targeting the other with a conditionally inactivating cassette allows rapid generation of conditional alleles.
2017-02-06A software tool, cryoSPARC, addresses the speed bottleneck in cryo-EM image processing, enabling automated macromolecular structure determination in hours on a desktop computer without requiring a starting model.
2017-01-18CROP-seq enables pooled CRISPR screens for complex transcriptome signatures by making gRNA expression detectable in single-cell RNA sequencing.
2017-01-30Single-cell combinatorial indexed sequencing (SCI-seq) resolves genomic heterogeneity by generating thousands of low-pass single-cell libraries at once for somatic copy number variant detection.
2017-01-23The Census tool converts single-cell RNA-seq relative read counts to relative transcript counts for more accurate differential gene expression and analysis in the absence of spike-ins or molecular barcodes.
2017-01-16The Microfluidic-based Ligand Enrichment (SMiLE) sequencing strategy probes DNA binding of single and heterodimeric transcription factors over a wide affinity range.