Subscribe: pubmed: "Allergology interna...
http://eutils.ncbi.nlm.nih.gov/entrez/eutils/erss.cgi?rss_guid=05vRiaq3JGvVzy2kHWxv08cpefLuv4Ax_MkYx0UTm-i
Added By: Feedage Forager Feedage Grade B rated
Language: English
Tags:
allergol int  eosinophil  eosinophils  expression  feb authors  human eosinophils  human  int feb  pmid pubmed  protease  prss  pubmed 
Rate this Feed
Rate this feedRate this feedRate this feedRate this feedRate this feed
Rate this feed 1 starRate this feed 2 starRate this feed 3 starRate this feed 4 starRate this feed 5 star

Comments (0)

Feed Details and Statistics Feed Statistics
Preview: pubmed: "Allergology interna...

pubmed: "Allergology interna...



NCBI: db=pubmed; Term=("Allergology international : official journal of the Japanese Society of Allergology"[Jour])



 



Corticosteroid use in urticaria multiforme cases.
Related Articles

Corticosteroid use in urticaria multiforme cases.

Allergol Int. 2017 Feb 16;:

Authors: Ercan N

PMID: 28216057 [PubMed - as supplied by publisher]




A successful case of egg allergy tolerance achieved at a local clinic.
Related Articles

A successful case of egg allergy tolerance achieved at a local clinic.

Allergol Int. 2017 Feb 16;:

Authors: Okada Y, Akasawa A

PMID: 28216056 [PubMed - as supplied by publisher]




Human eosinophils constitutively express a unique serine protease, PRSS33.
Related Articles

Human eosinophils constitutively express a unique serine protease, PRSS33.

Allergol Int. 2017 Feb 16;:

Authors: Toyama S, Okada N, Matsuda A, Morita H, Saito H, Fujisawa T, Nakae S, Karasuyama H, Matsumoto K

Abstract
BACKGROUND: Eosinophils play important roles in asthma, especially airway remodeling, by producing various granule proteins, chemical mediators, cytokines, chemokines and proteases. However, protease production by eosinophils is not fully understood. In the present study, we investigated the production of eosinophil-specific proteases/proteinases by transcriptome analysis.
METHODS: Human eosinophils and other cells were purified from peripheral blood by density gradient sedimentation and negative/positive selections using immunomagnetic beads. Protease/proteinase expression in eosinophils and release into the supernatant were evaluated by microarray analysis, qPCR, ELISA, flow cytometry and immunofluorescence staining before and after stimulation with eosinophil-activating cytokines and secretagogues. mRNAs for extracellular matrix proteins in human normal fibroblasts were measured by qPCR after exposure to recombinant protease serine 33 (PRSS33) protein (rPRSS33), created with a baculovirus system.
RESULTS: Human eosinophils expressed relatively high levels of mRNA for metalloproteinase 25 (MMP25), a disintegrin and metalloprotease 8 (ADAM8), ADAM10, ADAM19 and PRSS33. Expression of PRSS33 was the highest and eosinophil-specific. PRSS33 mRNA expression was not affected by eosinophil-activating cytokines. Immunofluorescence staining showed that PRSS33 was co-localized with an eosinophil granule protein. PRSS33 was not detected in the culture supernatant of eosinophils even after stimulation with secretagogues, but its cell surface expression was increased. rPRSS33 stimulation of human fibroblasts increased expression of collagen and fibronectin mRNAs, at least in part via protease-activated receptor-2 activation.
CONCLUSIONS: Activated eosinophils may induce fibroblast extracellular matrix protein synthesis via cell surface expression of PRSS33, which would at least partly explain eosinophils' role(s) in airway remodeling.

PMID: 28216055 [PubMed - as supplied by publisher]