Subscribe: pubmed: Thromb Haemost[jour]
http://eutils.ncbi.nlm.nih.gov/entrez/eutils/erss.cgi?rss_guid=0vRu_d0bpxUnGUrBWsFKzyVbC2pmlEJfowY_EGcPO_o
Added By: Feedage Forager Feedage Grade B rated
Language: English
Tags:
activity  coagulant activity  coagulant  density  drm  drms  haemost  membrane  plasma membrane  plasma  pubmed  thromb haemost  thromb 
Rate this Feed
Rate this feedRate this feedRate this feedRate this feedRate this feed
Rate this feed 1 starRate this feed 2 starRate this feed 3 starRate this feed 4 starRate this feed 5 star

Comments (0)

Feed Details and Statistics Feed Statistics
Preview: pubmed: Thromb Haemost[jour]

pubmed: Thromb Haemost[jour]



NCBI: db=pubmed; Term=Thromb Haemost[jour]



 



Lines of Zahn in the Splenic Vein.
Related Articles

Lines of Zahn in the Splenic Vein.

Thromb Haemost. 2018 Apr 21;:

Authors: Rashidi A, Gilles S, Linden MA

PMID: 29679949 [PubMed - as supplied by publisher]




Clinical Course of Venous Thromboembolism in Patients with Pancreatic Cancer: Insights from the RIETE Registry.
Related Articles

Clinical Course of Venous Thromboembolism in Patients with Pancreatic Cancer: Insights from the RIETE Registry.

Thromb Haemost. 2018 Apr 21;:

Authors: Frere C, Trujillo-Santos J, Font C, Sampériz Á, Quintavalla R, González-Martínez J, Vázquez FJ, Lima J, Farge D, Monreal M, RIETE Investigators

PMID: 29679948 [PubMed - as supplied by publisher]




Tissue Factor Coagulant Activity is Regulated by the Plasma Membrane Microenvironment.
Related Articles

Tissue Factor Coagulant Activity is Regulated by the Plasma Membrane Microenvironment.

Thromb Haemost. 2018 Apr 21;:

Authors: Yu Y, Böing AN, Hau CM, Hajji N, Ruf W, Sturk A, Nieuwland R

Abstract
BACKGROUND:  Tissue factor (TF) can be present in a non-coagulant and coagulant form. Whether the coagulant activity is affected by the plasma membrane microenvironment is unexplored.
OBJECTIVE:  This article studies the presence and coagulant activity of human TF in plasma membrane micro-domains.
METHODS:  Plasma membranes were isolated from human MIA PaCa2 cells, MDA-MB-231 cells and human vascular smooth muscle cells by Percoll gradient ultracentrifugation after cell disruption. Plasma membranes were fractionated by OptiPrep gradient ultracentrifugation, and the presence of TF, flotillin, caveolin, clathrin, protein disulphide isomerase (PDI), TF pathway inhibitor (TFPI) and phosphatidylserine (PS) were determined.
RESULTS:  Plasma membranes contain two detergent-resistant membrane (DRM) compartments differing in density and biochemical composition. High-density DRMs (DRM-H) have a density (ρ) of 1.15 to 1.20 g/mL and contain clathrin, whereas low-density DRMs (DRM-L) have a density between 1.09 and 1.13 g/mL and do not contain clathrin. Both DRMs contain TF, flotillin and caveolin. PDI is detectable in DRM-H, TFPI is not detectable in either DMR-H or DRM-L and PS is detectable in DRM-L. The DRM-H-associated TF (> 95% of the TF antigen) lacks detectable coagulant activity, whereas the DRM-L-associated TF triggers coagulation. This coagulant activity is inhibited by lactadherin and thus PS-dependent, but seemed insensitive to 16F16, an inhibitor of PDI.
CONCLUSION:  Non-coagulant and coagulant TF are present within different types of DRMs in the plasma membrane, and the composition of these DRMs may affect the TF coagulant activity.

PMID: 29679947 [PubMed - as supplied by publisher]