Preview: Forthcoming in Journal of Synchrotron Radiation
Forthcoming article in Journal of Synchrotron Radiation
Synchrotron radiation research is rapidly expanding with many new sources of radiation being created globally. Synchrotron radiation plays a leading role in pure science and in emerging technologies. The Journal of Synchrotron Radiation provides comprehen
OH cleavage from tyrosine: debunking a myth
A systematic MX investigation into the observed electron density loss around the –OH group of tyrosines, as a function of dose at 100 K, is reported. It is concluded that a probable explanation is aromatic ring disordering as opposed to –OH cleavage; occurrence of the latter mechanism is a misconception perpetuated in radiation damage literature, and is unsupported by any observations in radiation chemistry.
Development of a dose-limiting data collection strategy for serial synchrotron rotation crystallography
The best practice for dose-limiting serial synchrotron rotation crystallography was examined through anomalous signal and single-wavelength anomalous diffraction phasing of mercury-bound luciferin regenerating enzyme. Sample rotation enabled accurate data collection with fewer diffraction images than without rotation, and an increase in resolution and anomalous signal was observed up to 3.4 MGy even though specific damage occurred after an accumulated dose of 1.1 MGy.
X-ray fluorescence at nanoscale resolution for multicomponent layered structures: a solar cell case study
The study of multilayer stacks (e.g. thin-film solar cells) by X-ray fluorescence requires a thorough account of the depth-dependent attenuation of incident and excident X-ray beam intensity, as well as spatial thickness variations to achieve an accurate interpretation of the data. A procedure is presented that allows for the assessment of stoichiometric composition variations in high spatial resolution imaging by correcting for multiple effects.
A soft XAS transmission cell for operando studies
A new design of an operando soft X-ray transmission and fluorescence cell with capability of fast membrane replacement and easy operation compared to the previous designs is presented. The cell is suitable for studying solids, liquids and solid/liquid interfaces and manipulating the sample with electrochemical techniques and visible light.
Current status of the TwinMic beamline at Elettra: a soft X-ray transmission and emission microscopy station
A detailed description of the TwinMic beamline installed at Elettra, Italy, is presented. The beamline hosts a unique soft X-ray microscope supporting both transmission and scanning X-ray microscopy (TXM and STXM, respectively) within the one instrument. The beamline presently fosters the following techniques: STXM, TXM and ptychography, that can be combined with low-energy X-ray fluorescence and X-ray absorption near-edge structure spectroscopies. A detailed update of the design, operational modes and future upgrades of the TwinMic beamline is presented.
Effect of gamma irradiation on X-ray absorption and photoelectron spectroscopy of Nd-doped phosphate glass
Gamma irradiation was found to change the elemental concentration of the atoms in Nd-doped phosphate glass The intensity of the white line in the XANES spectrum correlated with the ratio O/Nd in the glass matrix. The ratio of non-bridging oxygen to total oxygen in the glass after gamma radiation correlated with the concentration of defects in the glass samples. Glasses having a higher concentration of oxygen were found to be soft to gamma irradiation.
Nuclear incorporation of iron during the eukaryotic cell cycle
Scanning X-ray fluorescence microscopy has been used to probe the distribution of S, P and Fe within cell nuclei at the new ID16 beamline. Estimates of P, S and mass signals for the chromosomal matter agree with quantitative X-ray phase contrast projection microscopy images of the same samples, while fluorescence shows Fe incorporation.
Measuring the criticality of the `magic condition' for a beam-expanding monochromator
The effect of minor mismatch between the geometric and single-ray foci for a cylindrically bent Laue double-crystal monochromator is examined and found to be less detrimental than previously believed. Even without exact matching, the transverse coherence of the X-ray beam is not deteriorated by the system, enabling the phase-based imaging techniques critical to modern biomedical imaging experiments.