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Forthcoming article in Acta Crystallographica Section F Structural Biology Communications



Acta Crystallographica Section F: Structural Biology Communications is a rapid all-electronic journal, which provides a home for short communications on the crystallization and structure of biological macromolecules. Structures determined through structur



 






Crystal structure of recombinant phosphoribosylpyrophosphate synthetase 2 from Thermus thermophilus HB27 complexed with ADP and sulfate ions
The crystal structure of PRPP synthetase from a thermophilic strain of T. thermophilus was determined. An ADP molecule and a sulfate ion were located in the active site and a sulfate ion was located in the allosteric site.



Phosphate-binding protein from Polaromonas JS666: purification, characterization, crystallization and sulfur SAD phasing
A phosphate-binding protein from Polaromonas JS666 has been isolated, purified, characterized and crystallized. As it shares only 31% sequence identity with its closest known homologous structure, molecular-replacement approaches failed and the structure was solved using the sulfur SAD method. Its structure is expected to reveal its binding cleft and explain its maximal phosphate-binding activity at basic pH.



Crystal structure of N-acetylmannosamine kinase from Fusobacterium nucleatum
The enzyme N-acetylmannosamine kinase (NanK) catalyzes the second step of the bacterial sialic acid catabolic pathway. Here, the structure of F. nucleatum NanK is presented at 2.23 Å resolution.



The crystal structure of human DEAH-box RNA helicase 15 reveals a domain organization of the mammalian DEAH/RHA family
DEAH-box RNA helicase 15 (DHX15) is important for both RNA metabolism and innate immune responses to viral RNA. The crystal structure of human DHX15, including the catalytic core consisting of the two N-terminal RecA domains and the C-terminal regulatory domains, was determined.



Crystal structure of the active form of native human thymidylate synthase in the absence of bound substrates
Apo human thymidylate synthase was crystallized using a low ammonium sulfate concentration. A sulfate ion at the interface between the two subunits favours the active conformation.



Crystal structure of the Thermoplasma acidophilum protein Ta1207
The protein of unknown function Ta1207 from T. acidophilum consists of two domains with a common novel α/β-fold. Ta1207 forms a homopentameric complex in the crystal lattice and in solution.



Crystallization and X-ray diffraction analysis of SpaE, a basal pilus protein from the gut-adapted Lactobacillus rhamnosus GG
The cell-wall-anchoring basal pilus protein SpaE from L. rhamnosus GG was crystallized using PEG 3350 as a precipitant to yield trigonal and orthorhombic crystal forms that diffracted to 3.1 and 1.5 Å resolution, respectively. Selenium-substituted SpaE crystals have been generated for Se-SAD phasing and structure determination.



Crystal structure of Rv1220c, a SAM-dependent O-methyltransferase from Mycobacterium tuberculosis
The crystal structure of Rv1220c reveals a typical methyltransferase fold and a His164–Asp138 pair which is conserved in homologues from all species of Mycobacterium.



Crystal structures of human Fabs targeting the Bexsero meningococcal vaccine antigen NHBA
The crystal structures and functional characterization of two anti-NHBA human Fabs provide insight into the recognition by human antibodies of one of the main components of the first vaccine against serogroup B N. meningitidis (Bexsero).