Subscribe: Forthcoming article in Acta Crystallographica Section F: Structural Biology and Crystallization Communications
http://journals.iucr.org/f/forthrss10.xml
Preview: Forthcoming article in Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Forthcoming article in Acta Crystallographica Section F Structural Biology Communications



Acta Crystallographica Section F: Structural Biology Communications is a rapid all-electronic journal, which provides a home for short communications on the crystallization and structure of biological macromolecules. Structures determined through structur



 



Crystal structure of truncated human coatomer protein complex subunit ζ1 (Copζ1)
Selective targeting of the ζ1 subunit of the human coatomer protein complex I (Copζ1) is a promising avenue for anticancer therapy, as knockouts of ζ1 have been shown to specifically kill both proliferating and nondividing tumour-cell populations. In order to provide a structural basis for rational drug design, the high-resolution crystal structure of Copζ1 is reported.



Characterization and 1.57 Å resolution structure of the key fire blight phosphatase AmsI from Erwinia amylovora
The high-resolution (1.57 Å) structure and kinetic data of the key fire blight phosphatase AmsI are reported.



Overcoming a hemihedral twinning problem in tetrahydrofolate-dependent O-demethylase crystals by the microseeding method
A hemihedral twinning problem was found in crystals of a tetrahydrofolate-dependent O-demethylase, LigM, from Sphingobium sp. SYK-6. The problem was overcome using microseeding.



The crystal structure of dihydrodipicolinate reductase from the human-pathogenic bacterium Bartonella henselae strain Houston-1 at 2.3 Å resolution
The cloning, expression, purification, crystallization and X-ray diffraction analysis of dihydrodipicolinate reductase from the human-pathogenic bacterium B. henselae, the causative bacterium of cat-scratch disease, are reported.



Coxsackievirus B3 protease 3C: expression, purification, crystallization and preliminary structural insights
The expression, purification, crystallization, X-ray powder diffraction data collection and preliminary analysis of protease 3C from coxsackievirus B3 are reported.



Purification, crystallization and characterization of the Pseudomonas outer membrane protein FapF, a functional amyloid transporter
FapF from Pseudomonas facilitates the secretion of the amyloid-forming polypeptide FapC across the bacterial outer membrane. A protocol for forming reproducible well diffracting crystals is described in which the switch to tetraethylene glycol monooctyl ether detergent during purification proved to be critical and represents a handy tip for β-barrel protein crystallography.



1.45 Å resolution structure of SRPN18 from the malaria vector Anopheles gambiae
The structure of the A. gambiae serine proteinase inhibitor (serpin) SRPN18 was determined, revealing an unusually short and constricted reactive-center loop.



Crystal structure of the toxin Msmeg_6760, the structural homolog of Mycobacterium tuberculosis Rv2035, a novel type II toxin involved in the hypoxic response
The crystal structure of Msmeg_6760 reveals a novel fold for a toxin that harbors a large central cavity that is likely to bind a large hydrophobic compound.



The crystal structure of the endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae
A cellulase from K. pneumoniae was cloned, expressed, purified and shown to be an endoglucanase. Its structure was determined at 1.76 Å resolution and comparisons with homologous enzymes provide information on substrate specificity.