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Forthcoming article in Acta Crystallographica Section F Structural Biology Communications



Acta Crystallographica Section F: Structural Biology Communications is a rapid all-electronic journal, which provides a home for short communications on the crystallization and structure of biological macromolecules. Structures determined through structur



 



The hidden treasure in your data: phasing with unexpected weak anomalous scatterers from routine data sets
Weak anomalous signals from serendipitously acquired surface-bound Ca atoms/native S atoms from routine data sets, not optimized for SAD phasing, were used to successfully phase protein structures.



New molecular packing in a crystal of pseudoazurin from Alcaligenes faecalis: a double-helical arrangement of blue copper
A new molecular packing of pseudoazurin from A. faecalis shows a right-handed double helix composed of blue copper sites.



Similarities in the structure of the transcriptional repressor AmtR in two different space groups suggest a model for the interaction with GlnK
The structure of ligand-free and DNA-unbound AmtR, a TetR-type global nitrogen regulator from C. glutamicum, was solved at 2.1 Å resolution in space group P21212 with six molecules in the asymmetric unit. A structure comparison with the previously solved structure of AmtR in a trigonal crystal system suggests a 6:6 stoichiometric association of three AmtR dimers with two trimeric GlnK complexes that prevents AmtR from binding to DNA and induces gene transcription.



Proteolysis of truncated hemolysin A yields a stable dimerization interface
Structural analysis of two variant forms of truncated hemolysin A reveals additional proteolysis of the C-terminal 31 amino acids under high-salt crystallization conditions. This structural plasticity may support the implementation of stably exposed, on-edge β-strands during template-assisted folding.



Crystallization of FcpA from Leptospira, a novel flagellar protein that is essential for pathogenesis
FcpA from Leptospira interrogans, which is essential for pathogenesis, was crystallized but proved to be recalcitrant to X-ray diffraction studies. The orthologue from the saprophyte L. biflexa allowed three different crystal forms to be obtained that were suitable for crystallographic studies.



The hyperthermophilic cystathionine γ-synthase from the aerobic crenarchaeon Sulfolobus tokodaii: expression, purification, crystallization and structural insights
Cystathionine γ-synthase, a pyridoxal 5′-phosphate (PLP)-dependent enzyme from the thermoacidophilic archaeon S. tokodaii, was crystallized under three conditions. The structural data suggested that the orientations of the PLP cofactor and the active-site residues differed among the three forms, which might be key to the activity of the enzyme under extreme conditions.



The crystal structure of mouse LC3B in complex with the FYCO1 LIR reveals the importance of the flanking region of the LIR motif
FYCO1 mediates microtubule plus-end-directed autophagosome transport by interacting with LC3. Here, the crystal structure of mouse LC3B in complex with the FYCO1 LC3-interacting region (LIR) motif peptide was determined.



Crystal structure of full-length Zika virus NS5 protein reveals a conformation similar to Japanese encephalitis virus NS5
The recent epidemic of Zika virus (ZIKV) has created a major global health concern. Here, the crystal structure of the full-length ZIKV NS5 protein at 3.05 Å resolution is presented to facilitate the structure-based design of antiviral agents against ZIKV.



Crystal structure of the type IV secretion system component CagX from Helicobacter pylori
The structure of the C-terminal domain of CagX is presented and structural comparisons with TraO, its homologue from another bacterial T4S system, reveal distinct and conserved features.