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Forthcoming article in Acta Crystallographica Section F Structural Biology Communications



Acta Crystallographica Section F: Structural Biology Communications is a rapid all-electronic journal, which provides a home for short communications on the crystallization and structure of biological macromolecules. Structures determined through structur



 



Crystallization and X-ray analysis of d-threonine aldolase from Chlamydomonas reinhardtii
Recombinant d-threonine aldolase from the green alga C. reinhardtii was purified to homogeneity and crystallized. An X-ray diffraction data set was collected to 1.85 Å resolution.



Crystal structure of the N-terminal anticodon-binding domain of the nondiscriminating aspartyl-tRNA synthetase from Helicobacter pylori
The crystal structure of the N-terminal anticodon-binding domain of the nondiscriminating aspartyl-tRNA synthetase from the human pathogen H. pylori was determined at a resolution of 2.0 Å. Two important turns (78GAGL81 and 83NPKL86), along with the conserved Pro84, are proposed to play a crucial role in the recognition of tRNAAsp and tRNAAsn.



Expression and crystallographic studies of d-glycero-β-d-manno-heptose-1-phosphate adenylyltransferase from Burkholderia pseudomallei
d-glycero-β-d-manno-Heptose-1-phosphate adenylyltransferase (HldC) from B. pseudomallei has been cloned, expressed, purified and crystallized. Synchrotron X-ray data from a selenomethionine-substituted HldC crystal were collected to 2.8 Å resolution.



Crystallization of a fungal lytic polysaccharide monooxygenase expressed from glycoengineered Pichia pastoris for X-ray and neutron diffraction
Polysaccharide monooxygenase 2 from N. crassa was expressed from a glycoengineered strain of P. pastoris. Crystals of the protein bearing modified N-linked glycans showed improved X-ray diffraction at 100 K (1.20 Å resolution) and permitted room-temperature neutron diffraction data collection to 2.12 Å resolution.



Structural characterization of the Streptococcus pneumoniae carbohydrate substrate-binding protein SP0092
The crystal structure of SP0092 was determined at 1.61 Å resolution and reveals a domain-swapped dimer with the monomer subunit in a closed conformation in the absence of ligand.



Expression, purification and crystallization of a protein resulting from the inversion of the amino-acid sequence of a helical bundle
To explore the sequence–structure relationships of four-α-helical bundles, the revRM6 protein, which has been engineered with the exact inverse amino-acid sequence of a hyperthermophilic helical bundle, was expressed, purified and crystallized and X-ray diffraction data were collected.



Structure of a fungal form of aspartate-semialdehyde dehydrogenase from Aspergillus fumigatus
The structure of a new fungal form of aspartate-semialdehyde dehydrogenase from A. fumigatus reveals differences in secondary-structural features that will be exploited for species-selective antibiotic development.



Recombinant hepatocyte growth factor activator inhibitor 1: expression in Drosophila S2 cells, purification and crystallization
The extracellular domain of human hepatocyte growth factor activator inhibitor 1 was expressed in Drosophila S2 cells. The recombinant protein was crystallized using ammonium sulfate as precipitant and the crystals diffracted to 3.8 Å resolution, with unit-cell parameters a = b = 95.42, c = 124.50 Å.



X-ray crystal structure of a malonate-semialdehyde dehydrogenase from Pseudomonas sp. strain AAC
The crystal structure of a malonate-semialdehyde dehydrogenase from Pseudomonas sp. strain AAC has been determined to a nominal resolution of 2.95 Å.



Crystal structure of a putrescine aminotransferase from Pseudomonas sp. strain AAC
The crystal structure of a putrescine aminotransferase from Pseudomonas sp. strain AAC has been determined to a resolution of 2.07 Å.



Crystal structure of the ATPase-C domain of the chromatin remodeller Fun30 from Saccharomyces cerevisiae
The C-terminal domain (residues 780–1122) of the ATPase motor Fun30 from S. cerevisiae has been crystallized and the structure was determined at a resolution of 1.95 Å. A helix-bundle insertion in the ATPase domain was identified to be specific to the Fun30 subfamily.



Crystal structure of an IclR homologue from Microbacterium sp. strain HM58-2
The crystal structure of an IclR homologue from Microbacterium sp. strain HM58-2 was determined at 2.1 Å resolution. It revealed a unique tetramer conformation, which may represent one of the multiple conformations in transcriptional regulation.



Crystal structure of truncated human coatomer protein complex subunit ζ1 (Copζ1)
Selective targeting of the ζ1 subunit of the human coatomer protein complex I (Copζ1) is a promising avenue for anticancer therapy, as knockouts of ζ1 have been shown to specifically kill both proliferating and nondividing tumour-cell populations. In order to provide a structural basis for rational drug design, the high-resolution crystal structure of Copζ1 is reported.