Subscribe: Forthcoming article in Acta Crystallographica Section F: Structural Biology and Crystallization Communications
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Preview: Forthcoming article in Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Forthcoming article in Acta Crystallographica Section F Structural Biology Communications

Acta Crystallographica Section F: Structural Biology Communications is a rapid all-electronic journal, which provides a home for short communications on the crystallization and structure of biological macromolecules. Structures determined through structur


Crystal structure of d(CCGGGGTACCCCGG)2 at 1.4 Å resolution
The structure of tetradecamer sequence d(CCGGGGTACCCCGG)2 is reported here as an A-DNA duplex at 1.4 Å resolution.

Structural insights into the mechanism of the drastic changes in enzymatic activity of cytochrome P450 vitamin D3 hydroxylase (CYP107BR1) caused by a mutation distant from the active site
Structures of the cytochrome P450 citamin D3 hydroxylase inactive mutants, F106V and L348M, were determined at 1.95 Å and 2.7 Å resolution, respectively.

Increasing the soluble expression and crystallization of the Escherichia coli quorum-sensing protein LsrK
LsrK from E. coli phosphorylates imported AI-2 molecules and triggers quorum-sensing signalling. The LsrK protein was expressed by applying heat and osmotic shock before IPTG induction, and the crystal of LsrK diffracted to 2.9 Å resolution.

X-ray diffraction analysis and in vitro characterization of the UAM2 protein from Oryza sativa
The UAM2 protein from O. sativa was cloned, expressed, purified and crystallized, and a complete data set was obtained from the radiation-sensitive crystals by low-dose vector data collection. In addition, it is shown that UAM2 is likely to exist as a monomer in solution and contains at least one intramolecular disulfide bridge or, alternatively, a structural metal ion.

Structure of NADP+-bound 7β-hydroxysteroid dehydrogenase reveals two cofactor-binding modes
The structure of a binary complex of NADP+ with 7β-hydroxysteroid dehydrogenase reveals two modes of cofactor binding.

Crystal structure of a small heat-shock protein from Xylella fastidiosa reveals a distinct high-order structure
The structure of XfsHSP17.9, a small heat-shock protein (sHSP) from X. fastidiosa, is similar to those of other sHSPs, having a conserved structural organization. The secondary structure of XfsHSP17.9 is mostly composed of β-strands and a few short α-helices. The XfsHSP17.9 structure reported here reveals a high-order architecture forming a nearly square cavity.

Crystal structure of enolase from Drosophila melanogaster
D. melanogaster enolase was purified and crystallized. It forms a homodimer with an open conformation.

X-ray crystal structure of human calcium-bound S100A1
The crystal structure of human Ca2+-bound S100A1 at 2.25 Å resolution is described.

Cryo-electron microscopy and X-ray crystallography: complementary approaches to structural biology and drug discoveryThis paper is an extension of Boutin et al. (2016).
Single-particle cryo-electron microscopy (cryo-EM) has gained its `lettres de noblesse' in structural biology. The synergistic convergence of technological and computational advances now make this a feasible method for determining structures at near-atomic to atomic resolution (∼5–2 Å). The potential impact of cryo-EM on drug discovery is discussed, together with how the method can provide information on conformational variability connected to the function of macromolecular complexes.

Structure of the complex of phosphorylated liver kinase B1 and 14-3-3ζ
The crystal structure of phosphorylated liver kinase B1 peptide in complex with 14-3-3ζ is reported.

Preliminary neutron diffraction analysis of challenging human manganese superoxide dismutase crystals
The growth of crystals of perdeuterated human manganese superoxide dismutase with a unit-cell edge of 240 Å was optimized for neutron data collection. Data were collected to 2.30 Å resolution from a 0.26 mm3 crystal. This is the crystal with the largest unit-cell edge from which data have been collected via neutron diffraction to sufficient resolution where hydrogen positions can be observed.

Crystal structure of the human heterogeneous ribonucleoprotein A18 RNA-recognition motif
The X-ray crystal structure of the RNA-recognition motif of human heterogeneous ribonucleoprotein A18 is reported at 1.77 Å resolution.

Crystallization and X-ray analysis of the extracellular adhesion domain of Helicobacter pylori adhesin A: the significance of the cation composition in the crystallization precipitant
The extracellular adhesion domain of H. pylori adhesin A was crystallized under two conditions using lithium sulfate and ammonium sulfate as the precipitant, which gave rise to two different crystal forms that diffracted to 1.95 and 2.6 Å resolution, respectively.

The hidden treasure in your data: phasing with unexpected weak anomalous scatterers from routine data sets
Weak anomalous signals from serendipitously acquired surface-bound Ca atoms/native S atoms from routine data sets, that were not optimized for SAD phasing, were used to successfully phase protein structures.