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Forthcoming article in Acta Crystallographica Section F Structural Biology Communications



Acta Crystallographica Section F: Structural Biology Communications is a rapid all-electronic journal, which provides a home for short communications on the crystallization and structure of biological macromolecules. Structures determined through structur



 



Crystallization and biochemical characterization of an archaeal lectin from Methanococcus voltae A3
An archaeal lectin from M. voltae A3 has been cloned, expressed, purified and crystallized, and crystals have been characterized using X-ray diffraction.



Crystal structure of a SAM-dependent O-methyltransferase from Mycobacterium tuberculosis
The crystal structure of Rv1220c reveals a typical methyltransferase fold and a His164–Asp138 pair which is conserved in homologues from all species of Mycobacterium.



Crystal structures of human Fabs targeting the Bexsero meningococcal vaccine antigen NHBA
The crystal structures and functional characterization of two anti-NHBA human Fabs provide insights into the recognition by human antibodies of one of the main components of the first vaccine against serogroup B N. meningitidis (Bexsero).



Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis
The structures of four nanobodies raised against the periplasmic domain of PorM were solved, and their interaction with their target was analysed. Diffracting crystals of two PorM constructs in complex with their specific nanobodies were obtained.



Crystal structure of CD27 in complex with a neutralizing noncompeting antibody
The crystal structure of human CD27 was determined at a resolution of 1.8 Å, revealing the assembly of the cysteine-rich domains. The binding of the antibody at the edge of the receptor molecule is unusual for the tumor necrosis factor receptor superfamily.



Transcription factor Rv0081 from Mycobacterium tuberculosis: purification, crystallization and initial crystallographic analysis
The response regulator Rv0081 is an essential regulatory hub for promoting the long-term survival of M. tuberculosis. Here, the production, crystallization and initial X-ray crystallographic analysis of Rv0081 are reported.



Crystal structure of the RNA 2′,3′-cyclic phosphodiesterase from Deinococcus radiodurans
The RNA 2′,3′-cyclic phosphodiesterase (CPDase) from D. radiodurans was crystallized and the crystals diffracted to 1.6 Å resolution. The structure, with an open active site, reveals an elegant mechanism for a CPDase enzyme, suggesting a conserved mechanism of catalysis.



Crystal structure of d(CCGGGGTACCCCGG)2 at 1.4 Å resolution
The structure of the tetradecamer sequence d(CCGGGGTACCCCGG)2 is reported here as an A-DNA duplex at 1.4 Å resolution.



Structural insights into the mechanism of the drastic changes in enzymatic activity of the cytochrome P450 vitamin D3 hydroxylase (CYP107BR1) caused by a mutation distant from the active site
The structures of inactive mutants (F106V and L348M) of the cytochrome P450 vitamin D3 hydroxylase were determined at 1.95 and 2.7 Å resolution, respectively.



Increasing the soluble expression and crystallization of the Escherichia coli quorum-sensing protein LsrK
LsrK from E. coli phosphorylates imported AI-2 molecules and triggers quorum-sensing signalling. The protein was expressed by applying heat and osmotic shock before IPTG induction, and a crystal of LsrK diffracted to 2.9 Å resolution.



Structure of NADP+-bound 7β-hydroxysteroid dehydrogenase reveals two cofactor-binding modes
The structure of a binary complex of NADP+ with 7β-hydroxysteroid dehydrogenase reveals two modes of cofactor binding.