Subscribe: Forthcoming article in Acta Crystallographica Section D: Biological Crystallography
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Forthcoming article in Acta Crystallographica Section D Structural Biology



Acta Crystallographica Section D: Structural Biology welcomes the submission of articles covering any aspect of structural biology, with a particular emphasis on the structures of biological macromolecules and the methods used to determine them. Reports o



 



Crystal structure of Yersinia pestis virulence factor YfeA reveals two polyspecific metal-binding sites
YfeA, a substrate-binding protein that is important for the virulence of Y. pestis, has two polyspecific metal-binding sites that may play different roles during infection. A flexible lobe at the carboxy-terminus suggests that structural rearrangement is required for metal transfer to binding partners.



Pepsi-SAXS: an adaptive method for rapid and accurate computation of small-angle X-ray scattering profiles
A new method called Pepsi-SAXS is presented that calculates small-angle X-ray scattering profiles from atomistic models. The method is based on the multipole expansion scheme and is significantly faster compared with the CRYSOL, FoXS and three-dimensional Zernike methods.






Crystal structure of the novel amino-acid racemase isoleucine 2-epimerase from Lactobacillus buchneri
Structural analysis of an isoleucine 2-epimerase from L. buchneri provided new insight into the catalytic mechanism of bacterial fold-type I racemases.






Cryo-electron microscopy of chromatin biology
This review article summarizes recent progress in our understanding of chromatin biology based on single-particle cryo-electron microscopy studies.



The first plant acyl-CoA-binding protein structures: the close homologues OsACBP1 and OsACBP2 from rice
The first structures of plant acyl-CoA-binding proteins, from rice, show similarity to the human liver and bovine homologues.



Trends in the Electron Microscopy Data Bank (EMDB)
The Electron Microscopy Data Bank (EMDB), the public archive for three-dimensional EM reconstructions, is an invaluable resource for obtaining a birds-eye view of trends affecting the field of cryo-EM. EMDB is growing rapidly, with almost a quarter of the entries having been released over the past year.



The potential use of single-particle electron microscopy as a tool for structure-based inhibitor design
Recent developments in electron microscopy have provided new opportunities in the field of structure-based drug design. This review looks at the challenges that remain and the future prospects for the use of electron microscopy in this area.



Structure of the family B DNA polymerase from the hyperthermophilic archaeon Pyrobaculum calidifontis
The structure of the family B DNA polymerase from the hyperthermophilic archaeon P. calidifontis has been determined with an R factor of 24.47% and an Rfree of 28.81% at 2.80 Å resolution. The enzyme has a number of unique structural features which distinguish it from other archaeal DNA polymerases.



Structural features of NS3 of Dengue virus serotypes 2 and 4 in solution and insight into RNA binding and the inhibitory role of quercetin
The NS3s of Dengue virus serotypes 2 and 4 are elongated and dynamic in solution. The protease domain of NS3 undergoes substantial conformational alterations owing to RNA binding. The interactions and effects of quercetin binding in NS3 are described.



Structural study of the X-ray-induced enzymatic reduction of molecular oxygen to water by Steccherinum murashkinskyi laccase: insights into the reaction mechanism
A series of diffraction data sets with increasing absorbed radiation dose were collected from a single crystal of Steccherinum murashkinskyi laccase at 1.35 Å resolution. The mechanism of oxygen reduction catalyzed by laccases is discussed.



The Dynamo package for tomography and subtomogram averaging: components for MATLAB, GPU computing and EC2 Amazon Web Services
A technical description of the Dynamo software package for subtomogram averaging is provided. Details are given on advanced MATLAB libraries, parallelization strategies, the use of GPUs and accessibility though the Amazon cloud computing services.



1.8 Å resolution crystal structure of the carbapenem intrinsic resistance protein CarF
The crystal structure of the carbapenem-resistance protein CarF has been determined at 1.8 Å resolution. CarF confers partial intrinsic resistance to the naturally occurring antibiotic 1-carbapen-2-em-3-carboxylic acid.