Subscribe: Forthcoming article in Acta Crystallographica Section D: Biological Crystallography
Preview: Forthcoming article in Acta Crystallographica Section D: Biological Crystallography

Forthcoming article in Acta Crystallographica Section D Structural Biology

Acta Crystallographica Section D: Structural Biology welcomes the submission of articles covering any aspect of structural biology, with a particular emphasis on the structures of biological macromolecules and the methods used to determine them. Reports o


Electron Bio-Imaging Centre (eBIC): the UK national research facility for biological electron microscopy
This paper provides an introduction to the Electron Bio-Imaging Centre (eBIC) at Diamond Light Source: an external user facility established in the UK for high-end cryo-electron microscopy. Details are given of the first year of operation along with highlights and future challenges.

Octamer formation in lysozyme solutions at the initial crystallization stage detected by small-angle neutron scattering
The lysozyme solution in heavy water with the precipitant addition was studied by the small angle neutron scattering (SANS) for lysozyme concentrations 40, 20, 10 mg/ml and temperatures 10 oC, 20 oC, and 30 oC. Above protein monomers also dimer and octamer oligomers have been found in solutions with maximal concentration near optimal crystallization conditions. The volume fractions of octamers are decreasing with the deviation from the optimal temperature and with the decrease of the protein concentration. Without the precipitant, only monomers are present in solution.

The ribosome and its role in protein folding: looking through a magnifying glass
The structural biology of co-translational protein folding on the ribosome is reviewed.

Probing the structural basis of oxygen binding in a cofactor-independent dioxygenase
The crystal structure of the cofactor-independent dioxygenase DpgC was refined and the new structure was used to probe the basis for dioxygen binding using site-directed mutagenesis.

High-resolution cryo-EM proteasome structures in drug development
High-resolution cryo-EM was used to investigate the structures of inhibitor-bound human and P. falciparum 20S proteasomes, revealing the molecular basis for inhibitor specificity that provides a platform for the development of a potential new class of antimalarials. Here, these studies are reviewed and a detailed description of the methods used for structure determination is provided.

Rapid cadmium SAD phasing at the standard wavelength (1 Å)
Single-wavelength anomalous dispersion (SAD) phasing experiments were successfully carried out at the standard wavelength of 1 Å by using cadmium ions as anomalous scatterers.

Crystal structure of Yersinia pestis virulence factor YfeA reveals two polyspecific metal-binding sites
YfeA, a substrate-binding protein that is important for the virulence of Y. pestis, has two polyspecific metal-binding sites that may play different roles during infection. A flexible lobe at the carboxy-terminus suggests that structural rearrangement is required for metal transfer to binding partners.

Cryo-electron microscopy of chromatin biology
This review article summarizes recent progress in our understanding of chromatin biology based on single-particle cryo-electron microscopy studies.

Trends in the Electron Microscopy Data Bank (EMDB)
The Electron Microscopy Data Bank (EMDB), the public archive for three-dimensional EM reconstructions, is an invaluable resource for obtaining a birds-eye view of trends affecting the field of cryo-EM. EMDB is growing rapidly, with almost a quarter of the entries having been released over the past year.

The potential use of single-particle electron microscopy as a tool for structure-based inhibitor design
Recent developments in electron microscopy have provided new opportunities in the field of structure-based drug design. This review looks at the challenges that remain and the future prospects for the use of electron microscopy in this area.

The Dynamo package for tomography and subtomogram averaging: components for MATLAB, GPU computing and EC2 Amazon Web Services
A technical description of the Dynamo software package for subtomogram averaging is provided. Details are given on advanced MATLAB libraries, parallelization strategies, the use of GPUs and accessibility though the Amazon cloud computing services.

1.8 Å resolution crystal structure of the carbapenem intrinsic resistance protein CarF
The crystal structure of the carbapenem-resistance protein CarF has been determined at 1.8 Å resolution. CarF confers partial intrinsic resistance to the naturally occurring antibiotic 1-carbapen-2-em-3-carboxylic acid.