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MedWorm: Candidosis provides a medical RSS filtering service. Over 7000 RSS medical sources are combined and output via different filters. This feed contains the latest news and research in the Candidosis category.

Last Build Date: Mon, 21 Mar 2016 22:19:54 +0100


Association of a variable number tandem repeat in the NLRP3 gene in women with susceptibility to RVVC

Mon, 07 Mar 2016 00:00:00 +0100

In this study, we assessed a variable number tandem repeat (VNTR) polymorphism in the NLRP3 gene that encodes a component of the inflammasome, processing the proinflammatory cytokines IL-1β and IL-18. A total of 270 RVVC patients and 583 healthy controls were analyzed, and increased diseases susceptibility was associated with the presence of the 12/9 genotype. Furthermore, functional studies demonstrate that IL-1β production at the vaginal surface is higher in RVVC patients bearing the 12/9 genotype compared to controls, whereas IL-1Ra levels were decreased and IL-18 levels remained unchanged. These findings suggest that IL-1β-mediated hyperinflammation conveyed by the NLRP3 gene plays a causal role in the pathogenesis of RVVC and may identify this pathway as a potential therapeutic tar...

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Vulvovaginal candidosis: contemporary challenges and the future of prophylactic and therapeutic approaches

Thu, 14 Jan 2016 04:46:41 +0100

Summary Vulvovaginal candidosis (VVC) is a common gynaecological disorder that is delineated by the inflammation of vaginal wall and it is caused by the opportunistic fungal pathogen Candida species. In fact, three out of every four women will experience at least one occasion of VVC during some point in their lives. Although uncomplicated VVC is relatively harmless, the complicated VVC such as recurrent attack often creates restlessness and depression in the patients, thus greatly affects their quality of life. Managements of VVC are usually associated with the use of antimycotic suppositories, topical cream or oral agents. These antimycotic agents are either available over‐the‐counter or prescribed by the clinicians. In recent decades, the rise of clinical challenges such as the incre...

Caspofungin‐induced in‐vitro post‐antifungal effect and its impact on adhesion related traits of oral Candida dubliniensis and Candida albicans isolates

Fri, 01 Jan 2016 00:00:00 +0100

ABSTRACT Adhesion to buccal epithelial cells (BEC) and denture acrylic surfaces (DAS), germ tube (GT) formation and cell surface hydrophobicity (CSH) are all virulence traits involved in pathogenicity of Candida. Post‐antifungal effect (PAFE) also have a bearing on pathogenicity and virulence of Candida. Candida dubliniensis is associated with oral and systemic candidosis, which can be managed with caspofungin. There is no information on caspofungin‐induced PAFE and its impact on adhesion traits of C. dubliniensis isolates. Thus, the purpose of this investigation was to determine the in vitro PAFE on 20 C. dubliniensis isolates following brief exposure to caspofungin. Furthermore the impact of caspofungin‐induced PAFE on adhesion to BEC and DAS, GT formation and CSH of these isolates...

The involvement of upper airway in Wegener's granulomatosis - about four cases.

Wed, 18 Nov 2015 20:16:05 +0100

Authors: Sarău CA, Lighezan DF, Doroş IC, Ştefănescu EH, Iovănescu G, Balica NC, Horhat ID, Poenaru M Abstract The authors present four cases of Wegener's granulomatosis patients with multiorganic manifestation forms, but with a prevalent involvement in upper-airway. Granulomatosis diseases of the nose include bacterial infections (rhinoscleroma, tuberculosis, syphilis, lupus, and leprosy), fungal infections (rhinosporidiosis, aspergillosis, mucormycosis, candidosis, histoplasmosis, and blastomycosis) and diseases with unspecified etiology (Wegener's granulomatosis, mediofacial malignant granuloma, and sarcoidosis). We consider an interesting experience regarding Wegener's granulomatosis due to its rarity, being an autoimmune systemic disease, with continuous evolution and mul...

Oral primary localized amyloidosis in HIV-infected patients: the oral face of a described skin lesion

Tue, 03 Nov 2015 00:00:00 +0100

Oral primary localized amyloidosis should be considered in the diagnosis of oral white lesions such as hyperplastic candidosis, lichen planus and lichenoid reactions; it is not associated with antiretroviral therapy use, systemic involvement or malignant transformation. (Source: International Journal of STD and AIDS)

M25 * Oral candidosis in the oncology department of the University Hospital in Pisa: prospective observational study

Mon, 26 Oct 2015 00:00:00 +0100

(Source: Annals of Oncology)

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Impact of brief exposure to antifungal agents on the post-antifungal effect and hemolysin activity of oral Candida albicans

Fri, 18 Sep 2015 17:47:09 +0100

Conclusions Brief exposure to sublethal concentrations of antifungal drugs appears to exert an antifungal effect by interfering with the growth as well as hemolysin production of C. albicans. (Source: Journal of Applied Oral Science)

Analysis of the biofilm formation of after Photodithazine-mediated photodynamic therapy in vivo

Tue, 01 Sep 2015 00:00:00 +0100

This study evaluated the capacity of biofilm formation of C. albicans recovered from the tongues of murine model of oral candidosis1, after consecutive applications of Photodithazine® (PDZ) (100mg/L) associated with LED (660nm) light (37.5J/cm2). Mice with oral candidosis were treated with photodynamic therapy (PDT) and Nystatin (NYS). Next, fungal cells were recovered from the oral lesions and submitted to biofilm formation. The capacity of biofilm formation was analyzed by the cellular viability (CFU/mL), XTT assay and Violet Crystal staining assay (CV). (Source: Photodiagnosis and Photodynamic Therapy)

Total biomass of multispecies-biofilm after Photodynamic Therapy mediated by nanoemulsion-phthalocyanine

Tue, 01 Sep 2015 00:00:00 +0100

This study evaluated the total biomass of multispecies-biofilm of Streptococcus mutans, Candida albicans and Candida glabrata submitted to Photodynamic Therapy (PDT) mediated by aluminum-chloride-phthalocyanine encapsulated in cationic nanoemulsion (FC-Cl-Al-NE) associated with 660nm-LED light. Standard suspensions (106–107CFU/mL) of these microorganisms were transferred to wells of microtiter plates for the multispecies-biofilm formation (48h at 37°C in candle jars). (Source: Photodiagnosis and Photodynamic Therapy)

A 52-year-old man with heartburn and weight loss

Thu, 09 Jul 2015 00:00:00 +0100

A previously fit and well 52-year-old man presented to the hospital with a 3-month history of refractory heartburn and progressive weight loss (5 kg). After an initial response to empiric treatment with 20 mg omeprazole daily, heartburn relapsed and became refractory to proton pump inhibitor. His past medical history and physical examination were unremarkable. Upper endoscopy showed a thick white membrane in the distal part of the oesophagus, crossing the gastro-oesophageal junction (figure 1A); the mucosa surrounding the distal margin was slightly depressed but not reddened. An initial diagnosis of candidosis was suggested and the patient unsuccessfully treated with nystatin. The biopsies showed no abnormalities, in particular no signs of inflammation. All laboratory tests, incl...

New Strategies for Local Treatment of Vaginal Infections.

Thu, 02 Jul 2015 00:00:00 +0100

Authors: Palmeira-de-Oliveira R, Palmeira-de-Oliveira A, Martinez-de-Oliveira J Abstract Vaginal infections are extremely prevalent, particularly among reproductive aged women. Although not resulting in high mortality, these infections are associated with high levels of anxiety and depreciation of low quality of life. In most cases, topical treatment of vaginal infections has been shown to be at least as effective as oral treatment, resulting in higher local drug concentrations, with less drug interactions and adverse effects. Furthermore, the emergence of microbial resistance to chemotherapeutics and the difficulties in managing infection recurrences sustain the need for more effective local treatments. However, conventional dosage forms have been associated with low retention in ...

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Organic Based Bio-sensor for Odor Detection in Gynecological Diseases

Sat, 06 Jun 2015 00:00:00 +0100

Publication date: 2015 Source:Materials Today: Proceedings, Volume 2, Issue 1 Author(s): Maria I. Marques , Joana Fonseca , João Gomes , Ana Palmeira-de-Oliveira , José Martinez-de-Oliveira , L. Pereira Odor sensors were fabricated by depositing organic polymeric semiconductor on screen-printed interdigitated electrodes. These sensors were tested with odors from different origins and in particular a culture of vaginal candidosis, in order the get insight about their use for a medical based diseases control. Both capacitive and resistive sensors were tested with different electrodes size/separations and composition. It was found that the carbon based electrodes with a size/separation of 300μm shows the best results with changes in on/off sate with a considerable amplitude suitable for ...

In vivo evaluation of photodynamic inactivation using Photodithazine[registered sign]against Candida albicans

Wed, 20 May 2015 17:04:15 +0100

This study describes the photoinactivation of Candida albicans in a murine model of oral candidosis, mediated by Photodithazine[registered sign] (PDZ). Six-week-old female Swiss mice were immunosuppressed, and inoculated with C albicans...The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Photochem. Photobiol. Sci. latest articles)

Impact of short‐term exposure of antifungal agents on hemolysin activity of oral Candida dubliniensis isolates from Kuwait and Sri Lanka

Fri, 01 May 2015 00:00:00 +0100

ConclusionBrief exposure to sublethal concentrations of drugs with antifungal properties appears to reduce the pathogenic potential of C. dubliniensis isolates by suppressing hemolysin production. (Source: Journal of Investigative and Clinical Dentistry)

Positive Family History, Infection, Low Absolute Lymphocyte Count (ALC), and Absent Thymic Shadow: Diagnostic Clues for All Molecular Forms of Severe Combined Immunodeficiency (SCID)

Mon, 30 Mar 2015 00:00:00 +0100

Conclusions Infants with SCID appear normal at birth but later present with failure to thrive and/or recurrent fungal, viral, and bacterial infections. Low ALCs and an absent thymic shadow on chest x-ray are key diagnostic clues. The absence of T-cell function confirms the diagnosis. (Source: The Journal of Allergy and Clinical Immunology: In Practice)

Nanoparticles for controlled delivery and sustained release of chlorhexidine in the oral environment

Fri, 20 Feb 2015 14:50:18 +0100

ConclusionsChlorhexidine hexametaphosphate nanoparticles increased both the local dose and duration of soluble chlorhexidine delivery when applied to hydroxyapatite surfaces. This may provide a means to deliver a sustained dose of chlorhexidine with less frequent interventions.This article is protected by copyright. All rights reserved. (Source: Oral Diseases)

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A novel Candida glabrata cell wall associated serine protease.

Wed, 21 Jan 2015 00:00:00 +0100

Authors: Pärnänen P, Meurman JH, Nikula-Ijäs P Abstract We set out to identify the Candida glabrata cell wall attached proteases which may play a role as virulence factors in candidosis, particularly in the immunocompromized host. We studied a clinical C. glabrata strain T-1639, which was isolated from a patient from the Helsinki University Central Hospital. With non-reducing 2-D electrophoresis using parallel fluorogenic gels and mass spectrometry we identified a novel appr. 25 kDa (192 aa in length) cell wall located protease with an estimated pI of 7.6. The LC-MS/MS peptides matched with the ORF of predicted C. glabrata CBS138 cell wall protein Cwp1.2p/pI 7.7/212 aa (énolevures/[NCBI access 49525604, UniProt access Q6FTZ7]), which is an ortholog to Sacc...

In vitro anti-Candida activity of lidocaine and nitroglycerin: alone and combined.

Tue, 18 Nov 2014 13:20:05 +0100

In conclusion, both drugs exhibited an interesting anti-Candida activity. The combination of lidocaine with nitroglycerin was shown to have an additive effect against Candida spp., predicting the interest to include, in the future, these drugs in a new delivery system for the treatment of mucocutaneous candidosis. PMID: 22675243 [PubMed - indexed for MEDLINE] (Source: Infectious Diseases in Obstetrics and Gynecology)

Pathogenesis and treatment of oral candidosis.

Sun, 16 Nov 2014 04:53:27 +0100

Authors: Williams D, Lewis M Abstract Oral infections caused by yeast of the genus Candida and particularly Candida albicans (oral candidoses) have been recognised throughout recorded history. However, since the 1980s a clear surge of interest and associated research into these infections have occurred. This has largely been due to an increased incidence of oral candidosis over this period, primarily because of the escalation in HIV-infection and the AIDS epidemic. In addition, changes in medical practice leading to a greater use of invasive clinical procedures and a more widespread use of immunosuppressive therapies have also contributed to the problem. Whilst oral candidosis has previously been considered to be a disease mainly of the elderly and very young, its occurrence throug...

Influence of daily immersion in denture cleanser on multispecies biofilm

Sat, 15 Nov 2014 14:43:07 +0100

Conclusions Daily exposure of a multispecies biofilm to a denture cleanser reduces the number of total microorganisms but favors C. albicans development. Clinical relevance Daily use of denture cleanser is an effective method for controlling bacteria in biofilm, but it can potentially select C. albicans, an important etiological agent of oral candidosis. (Source: Clinical Oral Investigations)

Impact of brief and sequential exposure to nystatin, amphotericin B, ketoconazole, and fluconazole in modulating adhesion traits of oral Candida dubliniensis isolates

Tue, 11 Nov 2014 00:00:00 +0100

ConclusionsBrief and sequential exposure of C. dubliniensis to antifungal agents would continue to wield an antifungal effect by altering its adhesion attributes, and elucidate possible pharmacodynamics by which antifungal agents might operate in modulating candidal adherence. (Source: Journal of Investigative and Clinical Dentistry)

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Novel Nystatin A1 derivatives exhibiting low host cell toxicity and antifungal activity in an in vitro model of oral candidosis

Fri, 26 Sep 2014 04:34:29 +0100

Abstract Opportunistic oral infections caused by Candida albicans are frequent problems in immunocompromised patients. Management of such infections is limited due to the low number of antifungal drugs available, their relatively high toxicity and the emergence of antifungal resistance. Given these issues, our investigations have focused on novel derivatives of the antifungal antibiotic Nystatin A1, generated by modifications at the amino group of this molecule. The aims of this study were to evaluate the antifungal effectiveness and host cell toxicity of these new compounds using an in vitro model of oral candidosis based on a reconstituted human oral epithelium (RHOE). Initial studies employing broth microdilution, revealed that against planktonic C. albicans, Nystatin A1 had l...

Presence of Candida cell wall derived polysaccharides in the sera of intensive care unit patients: relation with candidaemia and Candida colonisation

Sun, 29 Jun 2014 00:00:00 +0100

Conclusion: A combination of mannan and BDG tests could be used to guide pre-emptive therapeutic decisions in ICU patients. (Source: BioMed Central)

Detection of Candida albicans ADH1 and ADH2 mRNAs in human archival oral biopsy samples

Sat, 14 Jun 2014 00:00:00 +0100

ConclusionsCandida albicans was the predominant species in the lesions diagnosed as CHC, and the presence of C. albicans in CHC lesions was associated with a high expression of C. albicans ADH1 mRNA. There was no association between the presence of Candida and malignant transformation in the cases examined; however, the number of cases was limited and further studies are needed to further elucidate the role of C. albicans ADH1 in the pathogenesis of oral squamous cell carcinoma. (Source: Journal of Oral Pathology and Medicine)

Oral Budesonide as a Therapy for Protein‐Losing Enteropathy in Children After the Fontan Operation

Tue, 03 Jun 2014 00:00:00 +0100

ConclusionBudesonide can be used to treat protein‐losing enteropathy in selected patients with cardiac diseases. (Source: Journal of Cardiac Surgery)

Post‐antifungal effect and adhesion to buccal epithelial cells of oral Candida dubliniensis isolates subsequent to limited exposure to amphotericin B, ketoconazole and fluconazole

Thu, 22 May 2014 00:00:00 +0100

ConclusionsBrief exposure to sub‐lethal concentrations of aforementioned drugs would exert an antifungal effect by modifying the growth and adhesion of C. dubliniensis isolates. (Source: Journal of Investigative and Clinical Dentistry)

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Anti-Candida activity of fluoxetine alone and combined with fluconazole - A synergistic action against fluconazole-resistant strains.

Mon, 05 May 2014 00:00:00 +0100

Authors: Oliveira AS, Gaspar CA, Palmeira-de-Oliveira R, Martinez-de-Oliveira J, Palmeira-de-Oliveira A Abstract The purpose of this work was to determine the antimicrobial activity of fluoxetine, alone and combined with fluconazole, against twenty-nine Candida strains isolated from vulvovaginal candidosis.MIC and MLC ranged from 9.8 to 625 μg/mL for all strains tested. The combination of fluconazole with fluoxetine resulted in a synergistic activity in six Candida strains with FIX between 0.15-0.31. An indifferent effect was found for the remaining strains with FIX between 0.63-1. PMID: 24798281 [PubMed - as supplied by publisher] (Source: Antimicrobial Agents and Chemotherapy)

A23: Favorable Response to Belimumab in Childhood‐Onset Systemic Lupus Erythematosus

Thu, 27 Mar 2014 00:00:00 +0100

Conclusion:This is the first study investigating the use of belimumab in childhood‐onset SLE across major ethnic groups. Our data demonstrate favorable clinical outcomes including the discontinuation of steroids in 3/7 (43%) of our patients, and improvement in C3 and C4 as early as 3 months after the initiation of belimumab, suggesting a quicker onset of action for belimumab in children than that observed in adult studies, which may support a larger role for belimumab in childhood‐onset SLE. (Source: Arthritis and Rheumatism)

The postantifungal effect and phospholipase production of oral Candida albicans from smokers, diabetics, asthmatics, denture wearers and healthy individuals following brief exposure to subtherapeutic concentrations of chlorhexidine gluconate

Fri, 21 Mar 2014 23:43:38 +0100

Summary Candida albicans is the major aetiological agent of oral candidosis and one of its important virulent factors is the production of extracellular phospholipases, which can be modulated by subtherapeutic concentrations of antifungal agents thus decreasing their pathogenicity. Hence, considering that chlorhexidine gluconate (CG) is a common antimicrobial mouthwash used in dentistry and that its concentration in the mouth reaches subtherapeutic levels during dosage intervals due to the diluent effect of saliva and cleansing effect of the oral musculature, the postantifungal effect (PAFE) and the phospholipase production of oral C. albicans following brief exposure to subtherapeutic concentrations of CG was studied. Fifty C. albicans planktonic oral isolates obtained from smokers, dia...

Mucoadhesive Propolis Gel For Prevention Of Radiation-Induced Oral Mucositis.

Wed, 05 Feb 2014 00:00:00 +0100

The objective of this phase II study was to determine the effectiveness of a mucoadhesive propolis gel in the prevention of radiation-induced oral mucositis. Twenty-four patients who were selected to undergo radiation therapy for oral cancer were included in this open-label trial. They were advised to use a mucoadhesive gel containing propolis 5,0% w/v three times a day starting one day before the course of radiation therapy and concluding after 2 weeks of radiation therapy. A weekly follow-up for evaluation of food intake, pain and grading of mucositis was performed. In order to confirm the absence of Candida-related mucositis in patients who developed mucositis, it was performed exfoliative cytology of buccal mucosa, palate and tongue and the material for Candifast® Candida species iden...

Addition to inhaled corticosteroids of long-acting beta2-agonists versus anti-leukotrienes for chronic asthma.

Fri, 24 Jan 2014 05:00:00 +0100

CONCLUSIONS: In adults with asthma that is inadequately controlled by predominantly low-dose ICS with significant bronchodilator reversibility, the addition of LABA to ICS is modestly superior to the addition of LTRA in reducing oral corticosteroid-treated exacerbations, with an absolute reduction of two percentage points. Differences favouring LABA over LTRA as adjunct therapy were observed in lung function and, to a lesser extend, in rescue medication use, symptoms and quality of life. The lower overall withdrawal rate and the higher proportion of participants satisfied with their therapy indirectly favour the combination of LABA + ICS over LTRA + ICS. Evidence showed a slightly increased risk of SAE with LABA compared with LTRA, with an absolute increase of one percentage point. Our fin...

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Efficiency at the Resident's Level of the NABUCCOD Nutrition and Oral Health Care Training Program in Nursing Homes

Mon, 06 Jan 2014 00:00:00 +0100

Conclusions: Training program improved staff knowledge with positive results at the level of residents but no significant changes were seen within NH policies. (Source: Journal of the American Medical Directors Association)

Candida spp. adherence to oral epithelial cells and levels of IgA in children with orthodontic appliances

Sun, 22 Dec 2013 09:05:07 +0100

In conclusion, orthodontic appliances may favor the adherence of Candida to epithelial cells but do not influence the presence of these yeasts in saliva, and the levels of anti-C. albicans IgA do not correlate with yeast adherence or presence of Candida in the oral cavity (Source: Brazilian Oral Research)

Oral candidosis in relation to oral immunity

Wed, 09 Oct 2013 04:00:00 +0100

Symptomatic oral infection with Candida albicans is characterized by invasion of the oral epithelium by virulent hyphae that cause tissue damage releasing the inflammatory mediators that initiate and sustain local inflammation. Candida albicans triggers pattern‐recognition receptors of keratinocytes, macrophages, monocytes and dendritic cells, stimulating the production of IL‐1β, IL‐6 and IL‐23. These cytokines induce the differentiation of Th17 cells and the generation of IL‐17‐ and/or IL‐22‐mediated antifungal protective immuno‐inflammatory responses in infected mucosa. Some immune cells including NKT cells, γδ T cells and lymphoid cells that are innate to the oral mucosa have the capacity to produce large quantities of IL‐17 in response to C. albicans, sufficient...


Tue, 01 Oct 2013 04:00:00 +0100

Praxis (Bern 1994). 2013 Oct 1;102(20):1251-1255 Authors: Lorenz J, Vollenweider P, Vuilleumier H, Schwab M Abstract Esophageal intramural pseudodiverticulosis is a rare pathology whose etiology is unknown, but which is frequently associated with three highly prevalent entities: esophageal reflux disease, esophageal candidosis and alcoholic esophagitis. With conservative treatment the course of these pathologies is usually benign. However, some severe cases are resistant to conservative treatment and may require more aggressive management. We here present the case of patient suffering from a severe esophagitis complicated by chronic mediastinitis with life-threatening repercussions, requiring esophagectomy as treatment. PMID: 24088236 [PubMed - as supplied by publisher] (So...

Detection of (1,3)-β-D-glucans in situ in a Candida albicans brain granuloma

Mon, 12 Aug 2013 04:00:00 +0100

(1, 3)-β-D-glucan (BDG), a major fungal cell wall component, is a useful biomarker of invasive fungal infections (IFI), particularly candidosis, aspergillosis and pneumocystosis. The European Organization for the Research and Treatment of Cancer/Mycoses Study Group (EORTC/MSG) included this test for the diagnosis of IFI using serum samples. Nowadays, BDG is a helpful non-invasive biomarker to screen patients at high risk of IFI. Some studies have reported BDG detection in other samples: bronchoalveolar lavage, cerebrospinal and peritoneal fluid. To our knowledge, no studies have dealt directly with BDG testing in tissues. (Source: Journal of Infection)

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The postantifungal effect of nystatin and its impact on adhesion attributes of oral Candida dubliniensis isolates

Mon, 17 Jun 2013 04:00:00 +0100

Summary The postantifungal effect (PAFE) has an impact on candidal pathogenicity. However, there is no information on either the PAFE or its impact on adhesion traits of oral Candida dubliniensis isolates. Oral candidosis can be treated topically with nystatin. Adhesion to buccal epithelial cells (BEC), germ tube (GT) formation and relative cell surface hydrophobicity (CSH) are all colonisation attributes of candidal pathogenicity. Hence, the main objective of this study was to investigate the in vitro PAFE on 20 C. dubliniensis isolates following exposure to nystatin. In addition, the impact of nystatin‐induced PAFE on adhesion to BEC, GT formation and relative CSH of C. dubliniensis isolates were also evaluated. After determining the minimum inhibitory concentration (MIC) of nystatin, ...

Structure-Antifungal Activity Relationships of Polyene Antibiotics of the Amphotericin B Group.

Tue, 28 May 2013 04:00:00 +0100

Authors: Tevyashova AN, Olsufyeva EN, Solovieva SE, Printsevskaya SS, Reznikova MI, Trenin AS, Galatenko OA, Treshalin ID, Pereverzeva ER, Mirchink EP, Isakova EB, Zotchev SB, Preobrazhenskaya MN Abstract A comprehensive comparative analysis of the structure - antifungal activity relationships for the series of biosynthetically engineered nystatin analogues, their novel semisynthetic derivatives, as well as amphotericin B (AMB) and its semisynthetic derivatives was performed. The data obtained revealed the significant influence of the structure of the C7 - C10 polyol region on the antifungal activity of these polyene antibiotics. Comparison of positions of hydroxyl groups in the antibiotics and in vitro antifungal activity data showed that the most active are the compounds in which...

Oral Candida species in head and neck cancer patients treated by radiotherapy

Wed, 01 May 2013 13:11:24 +0100

Conclusions: Diversification and major prevalence of non-albicans species were observed in irradiated patients on head and neck and this treatment is straightly associated with fungus positiveness. (Source: Auris, Nasus, Larynx)

Effect of Diamond‐Like Carbon Thin Film Coated Acrylic Resin on Candida albicans Biofilm Formation

Mon, 01 Apr 2013 04:00:00 +0100

Conclusion: Diamond‐like carbon films, doped or undoped with silver nanoparticles, coating the base of PMMA‐based dentures could be an alternative procedure for preventing candidosis in denture users. (Source: Journal of Prosthodontics)

Experience with the Platelia Candida ELISA for the diagnosis of invasive candidosis in neonatal patients

Tue, 05 Feb 2013 09:15:12 +0100

Oliveri S, Trovato L, Betta P, Romeo MG, Nicoletti G (Source: The Aspergillus Website - articles)

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Fermentative 2‐carbon metabolism produces carcinogenic levels of acetaldehyde in Candida albicans

Sat, 19 Jan 2013 19:45:02 +0100

Summary Acetaldehyde is a carcinogenic product of alcohol fermentation and metabolism in microbes associated with cancers of the upper digestive tract. In yeast acetaldehyde is a by‐product of the pyruvate bypass that converts pyruvate into acetyl‐CoA during fermentation. The aims of our study were: i) to determine the levels of acetaldehyde produced by C. albicans in the presence of glucose in low oxygen tension in vitro; ii) to analyze the expression levels of genes involved in the pyruvate‐bypass and acetaldehyde production; iii) to analyze whether any correlations exist between acetaldehyde levels, Adh enzyme activity or expression of the genes involved in the pyruvate‐bypass. C. albicans strains were isolated from patients with oral squamous cell carcinoma (n=5), APECED patien...

Changes in germ tube formation and cell‐surface hydrophobicity of oral Candida dubliniensis isolates following brief exposure to sub‐cidal concentrations of polyene and azole antifungal agents

Tue, 01 Jan 2013 05:00:00 +0100

The objective of this study was to investigate the effect of brief exposure to sub‐lethal concentrations of these antifungals on the germ tube formation and CSH of C. dubliniensis. After determining the minimum inhibitory concentration of the drugs, 20 oral isolates of C. dubliniensis were exposed to sub‐lethal concentrations of these antifungals for 1 h. Following this brief exposure, the drugs were removed, and following subsequent incubation in a germ tube inducing medium and exposure to bi‐phasic hydrocarbon assay, the germ tube formation and CSH of these isolates was quantified respectively. Compared with controls, exposure to amphotericin B almost completely suppressed the ability to form germ tubes with a mean percentage reduction of 95.91% (P < 0.0001), whereas ketocona...

Hydroxyurea therapy in sickle cell anemia patients aids to maintain oral fungal colonization balance

Wed, 26 Dec 2012 05:00:00 +0100

ConclusionHydroxyurea therapy seems to decrease candidal counts and resistance rate in sickle cell anemia patients. However, further studies should be conducted in the future to confirm this finding. Hydroxyurea therapy in sickle cell anemia patients maintains fungal species balance in oral cavity. (Source: Journal of Oral Pathology and Medicine)

Occupational exposures to aluminum and iron and risk of lung epithelium atypia in sudan

Mon, 24 Dec 2012 10:46:40 +0100

This study investigated the risk of lung cytological atypical changes in regards to occupational exposure to aluminum and iron. Detailed job histories were elicited from 130 incident cases with confirmed exposure to aluminum (50) or iron (80) and 157 population controls (nonexposed). Cytological atypia in sputum (dysplasia) was identified in four cases and none of controls (RR =10.8550; 95% CI = 0.5898 to 199.7815, P = 0.1086), hence, metaplasia was observed among 15 (11.5%) of the cases and 10 (6%) of controls (RR = 1.8115; 95% CI = 0.8424–3.8956; P = 0.1283). Evidences of viral infection were observed in 18 (14%) of the cases and 8 (5%) of controls (RR = 2.7173; 95% CI = 1.2213–6.0460; P = 0.0143). Moniliasis was observed in 28 (22%) of the cases and 19 (12%) of controls (RR = 1.6632...

Neonatal candidosis: clinical picture, management controversies and consensus, and new therapeutic options

Wed, 19 Dec 2012 21:38:33 +0100

Leibovitz E (Source: The Aspergillus Website - articles)

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Association of T916C (Y257H) mutation in Candida albicans ERG11 with fluconazole resistance

Mon, 10 Dec 2012 05:00:00 +0100

Summary Repeated and prolonged use of fluconazole in treating candidosis leads to drug resistance. The aim of this study was to confirm the relationship between T916C (Y257H) mutation in Candida albicans ERG11 and fluconazole resistance. We replaced one copy of ERG11 with ERG11 containing the T916C mutation in C. albicans CAI4 and expressed ERG11 with the T916C mutation in Saccharomyces cerevisiae INVSc1. The MIC values were two‐ to four‐fold greater in CAI4 transformants with than without the T916C mutation and 128 and 32 μg ml−1 for S. cerevisiae INVSc1‐containing ERG11 with and without the T916C mutation. T916C mutation may be associated with fluconazole resistance in C. albicans. (Source: Mycoses)

Disseminated aspergillosis and moniliasis associated with agranulocytosis and antibiotic therapy

Mon, 19 Nov 2012 20:43:22 +0100

RANKIN NE (Source: The Aspergillus Website - articles)

Recurrent vulvovaginal candidosis among young women in south eastern Nigeria: the role of lifestyle and health-care practices

Thu, 25 Oct 2012 04:00:00 +0100

This work was carried out to study the association between lifestyle, health-care practices and incidence of recurrent vulvovaginal candidosis (RVVC) among young women in south eastern Nigeria. It was a multicentre case-control study of 176 women aged between 20 and 35 years recruited from the designated health facilities. Lifestyle and health-care practice structured questionnaires were used to survey the participants. Clinical examination was performed and vaginal swabs were taken from participants for microscopy, culture and sensitivity. The germ tube test was performed for identification of Candida albicans. The results of this study showed that frequent douching with water or other fluids (odds ratio [OR]adjusted = 2.41, 95% confidence interval [CI] = 1.25–4.66, P = 0.000), wear...

Chlorhexidine is a highly effective topical broad-spectrum agent against Candida spp.

Fri, 19 Oct 2012 04:00:00 +0100

In conclusion, the findings showed that CHX has excellent broad-spectrum antifungal activity in vitro. It was effective at concentrations detected in saliva when using standard dosing regimens. Moreover, no cross-resistance was detected between CHX and FLZ, even among Candida spp. highly resistant to FLZ. (Source: International Journal of Antimicrobial Agents)

Effects of probiotic bacteria on Candida presence and IgA anti-Candida in the oral cavity of elderly.

Mon, 01 Oct 2012 04:00:00 +0100

In conclusion, probiotic bacteria reduced Candida numbers in the oral cavity of the elderly and increased specific secretory immune response against these yeasts, suggesting its possible use in controlling oral candidosis. PMID: 23306230 [PubMed - in process] (Source: Braz Dent J)

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Oral Candida colonization and candidiasis in patients with psoriasis

Fri, 28 Sep 2012 04:00:00 +0100

The objective of this study was to investigate oral Candida colonization and candidosis in a group of patients with psoriasis and controls. Study Design: A total of 100 patients with psoriasis and matched controls underwent the concentrated oral rinse test for Candida isolation. Candida species were identified by the VITEK 2 Identification System. Categorical variables were evaluated using the χ2 test. The median Candida count was compared using the Mann-Whitney U test. Results: Oral candidiasis was diagnosed in 3% of the patients with psoriasis. The Candida count and prevalence were significantly higher in the patients with psoriasis compared with controls (69% vs 44%, P < .001), but with no relationship to the severity or treatment of psoriasis. Oral Candida was significantly higher...

[Case Report] The tainted milk of human kindness

Fri, 17 Aug 2012 06:50:06 +0100

In September, 2009, a 74-day-old infant presented to a local hospital in Bloemfontein, South Africa, in bronchopneumonia related respiratory distress. She appeared pale, cyanosed, and had palpable axillary lymph nodes, hepatomegaly, and oropharyngeal candidosis. She had received all scheduled vaccinations and had been exclusively breastfed. Cytomegalovirus was isolated from a respiratory sample by shell vial culture, and a paired blood sample showed a cytomegalovirus viral load of 12 600 copies per mL. (Source: LANCET)

Urban legends series: oral candidosis

Tue, 14 Aug 2012 23:40:04 +0100

Abstract Candida species (spp) are commensal yeast that can only instigate oral infection (oral candidosis ‐ OC) when there is an underlying predisposing condition in the host. We investigated four controversial topics on OC : (1) How can a microbiological determination of OC be made since Candida spp. are commensal yeasts and not all of them form hyphae or pseudohyphae during infection? (2) Is median rhomboid glossitis (MRG) a manifestation of candidal infection? (3) Can candidal infection cause palate papillary hyperplasia (PPH)? (4) What is the best therapeutic treatment for denture‐associated erythematous stomatitis (DAES)? Results from extensive literature searches, including a systematic review, suggested the following: (1) The diagnosis OC merely on the basis of the presence of ...

[Efficacy of voriconazole treatment in chronic vulvovaginal candidosis due to Candida glabrata.]

Wed, 08 Aug 2012 04:00:00 +0100

CONCLUSIONS: These results suggest that the use of voriconazole as a therapeutic alternative for this type of candidosis which, although not life threatening, is associated with a high morbidity. PMID: 22885618 [PubMed - as supplied by publisher] (Source: Revista Iberoamericana de Micologia)

Oral candidosis in lichen planus: the diagnostic approach is of major therapeutic importance

Fri, 15 Jun 2012 07:39:05 +0100

Conclusion  This study highlights the importance of lesional sampling in OLP patients with suspected OC. Clinical relevance  Correct fungal identification is critical in order to initiate adequate antimycotic therapy, thus minimizing iatrogen selection of non-albicans species. Content Type Journal ArticleCategory Original ArticlePages 1-9DOI 10.1007/s00784-012-0757-6Authors C. Kragelund, Department of Oral Medicine, Section of Oral Pathology & Medicine, Institute of Odontology, University of Copenhagen, Norre Alle 20, DK-2200 Copenhagen N, DenmarkL. Kieffer-Kristensen, Department of Oral Medicine, Section of Oral Pathology & Medicine, Institute of Odontology, University of Copenhagen, Norre Alle 20, DK-2200 Copenhagen N, DenmarkJ. Reibel, Department o...

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Evaluation of novel nystatin nanoemulsion for skin candidosis infections

Fri, 11 May 2012 13:06:47 +0100

SummaryOne of the most common fungal skin infections is candidosis. Topical application of drugs at the pathological sites offers potential advantage of direct drug delivery to the site of action. The main aim of this work was to evaluate an optimal nystatin nanoemulsion for topical application avoiding undesirable side effects as systemic absorption and toxicity. Surface morphology and droplet size distribution of nystatin nanoemulsion was determined by transmission electronic microscopy and dynamic light scattering. Vertical diffusion Franz‐type cells and high‐performance liquid chromatography were used to perform the in vitro release and ex vivo human skin permeation studies. Transdermal permeation parameters were estimated from the permeation values using different theoretical appr...

Guideline vulvovaginal candidosis (2010) of the german society for gynecology and obstetrics, the working group for infections and infectimmunology in gynecology and obstetrics, the german society of dermatology, the board of german dermatologists and the german speaking mycological society

Mon, 23 Apr 2012 04:00:00 +0100

AbstractCandida (C.) species colonize the estrogenized vagina in at least 20% of all women. This statistic rises to 30% in late pregnancy and in immunosuppressed patients. The most often occurring species is Candida albicans.Host factors, especially local defense deficiencies, gene polymorphisms, allergic factors, serum glucose levels, antibiotics, psychosocial stress and estrogens influence the risk for a Candida vulvovaginitis. In less than 10% of all cases, non‐albicans species, especially C. glabrata, but in rare cases also Saccharomyces cerevisiae, cause a vulvovaginitis, often with fewer clinical signs and symptoms.Typical symptoms include premenstrual itching, burning, redness and non‐odorous discharge. Although pruritus and inflammation of the vaginal introitus are typical symp...

Kwashiorkor and coagulation disturbance: atypical presentation of cystic fibrosis

Fri, 30 Mar 2012 20:03:13 +0100

OBJETIVO: Enfatizar a apresentação clínica precoce da fibrose cística (FC) em lactente com Kwashiorkor e distúrbio de coagulação, decorrente de hipovitaminose K. DESCRIÇÃO DO CASO: Paciente com três meses e meio, sexo feminino, nascida a termo, peso de 2655g, estatura de 46cm, foi encaminhada para investigação de lesões perineais associadas à monilíase de difícil controle, refratária a diversos antifúngicos e corticoides. Quadro geral caracterizado por baixo ganho ponderal, edema e diarreia. Admissão hospitalar para investigação com hipótese diagnóstica de Kwashiorkor de origem primária ou secundária. Paciente mantida em aleitamento materno exclusivo, sendo observadas perda ponderal e persistência da diarreia. Na internação, foi iniciado tratamento de infecção...

Frequency and expression of ALS and HWP1 genotypes in Candida albicans strains isolated from Mexican patients suffering from vaginal candidosis

Mon, 19 Mar 2012 04:00:00 +0100

SummaryTo detect the frequency and expression of eight ALS (agglutinin‐like sequence) genes and the HWP1 genotype in a group of Candida albicans strains isolated from Mexican women suffering from vaginal candidosis. A group of 264 women (age 15–57 years) with vaginal infections were evaluated. C. albicans was identified by PCR amplification of the rRNA internal transcribed spacer regions ITS1 and ITS2. The ALS and HWP1 genes were identified by conventional PCR, and their expression levels were determined by real‐time PCR after growing C. albicans strains in reconstituted human vaginal epithelium (RHVE). C. albicans was identified in 50 women (18.9%). The genotypic frequencies were ALS1 100%, ALS2 60%, ALS3 36%, ALS4 54%, ALS5 70%, ALS6 56%, ALS7 64%, ALS9 66% and HWP1 92%. The most...

New insights into hepatosplenic candidosis, a manifestation of chronic disseminated candidosis

Sat, 25 Feb 2012 11:50:25 +0100

This article will focus on the clinical, diagnostic and therapeutic aspects of HSC and provide an accurate review of recent pathophysiological data. (Source: Mycoses)

Low virulent oral Candida albicans strains isolated from smokers

Sun, 29 Jan 2012 16:46:12 +0100

Abstract: It is widely accepted that tabagism is a predisposing factor to oral candidosis and cumulate data suggest that cigarette compounds may increase candidal virulence. To verify if enhanced virulence occurs in Candida albicans from chronic smokers, a cohort of 42 non-smokers and other of 58 smokers (all with excellent oral conditions and without signs of candidosis) were swabbed on tong dorsum and jugal mucosa. Results showed that oral candidal loads do not differ between smoker and non-smokers. Activities of secreted aspartyl-protease (Sap), phospholipase, chondroitinase, esterase–lipase, and haemolysin secretions were screened for thirty-two C. albicans isolates. There were detected significant increments in phospholipasic and chondroitinasic activities in isolates from non-smoke...

Oral sequelae of head and neck radiotherapy

Mon, 26 Dec 2011 03:42:57 +0100

CONCLUSION: regular dental follow-up may reduce the effects of radiation in the tissues of the oral cavity. (Source: Revista CEFAC)

Diagnosis of chronic disseminated candidosis from liver biopsies by a novel PCR in patients with haematological malignancies

Wed, 30 Nov 2011 05:00:00 +0100

In conclusion, the novel LCD chip technique examined in our study was able to detect fungal pathogens in liver biopsies from patients with haematological malignancies and suspected HCI/CDC but was negative in control biopsies. (Source: Clinical Microbiology and Infection)

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Insights into Candida tropicalis nosocomial infections and virulence factors

Sun, 30 Oct 2011 05:37:59 +0100

Abstract   Candida tropicalis is considered the first or the second non-Candida albicans Candida (NCAC) species most frequently isolated from candidosis, mainly in patients admitted in intensive care units (ICUs), especially with cancer, requiring prolonged catheterization, or receiving broad-spectrum antibiotics. The proportion of candiduria and candidemia caused by C. tropicalis varies widely with geographical area and patient group. Actually, in certain countries, C. tropicalis is more prevalent, even compared with C. albicans or other NCAC species. Although prophylactic treatments with fluconazole cause a decrease in the frequency of candidosis caused by C. tropicalis, it is increasingly showing a moderate level of fluconazole resistance. The propensity of C. tropicalis f...

Antifungal activity of phenolic-rich Lavandula multifida L. essential oil

Fri, 21 Oct 2011 16:01:31 +0100

This study evaluates the antifungal activity and mechanism of action of a new chemotype of Lavandula multifida from Portugal. The essential oil was analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS), and the minimal inhibitory concentration (MIC) and minimal lethal concentration (MLC) of the oil and its major compounds were determined against several pathogenic fungi responsible for candidosis, meningitis, dermatophytosis, and aspergillosis. The influence of the oil on the dimorphic transition in Candida albicans was also studied, as well as propidium iodide (PI) and FUN-1 staining of C. albicans cells by flow cytometry. The essential oil was characterized by high contents of monoterpenes, with carvacrol and cis-β-ocimene being the main constituents. T...

Oral candidosis - Clinical challenges of a biofilm disease.

Sat, 08 Oct 2011 16:38:40 +0100

Authors: Rautemaa R, Ramage G Abstract This review summarizes the impact of biofilms in oral candidosis with special emphasis on medically compromised patients. The concept of oral candidosis as a mixed candidal-bacterial biofilm infection has changed our understanding of its epidemiology and diagnosis as well as approach to its treatment. Candida albicans is the most common causative agent of oral candidosis although Candida species other than C. albicans are often seen in medically compromised patients with a history of multiple courses of azole antifungals. Although C. albicans is usually susceptible to all commonly used antifungals when tested in vitro, their biofilm form are highly resistant to most antifungals. Therefore, treatment consists of mechanical destruction of the bi...

Use of Dermasilk briefs in recurrent vulvovaginal candidosis: safety and effectiveness

Mon, 05 Sep 2011 04:00:00 +0100

SummaryDespite the generally excellent results achieved with fluconazole 150 mg weekly in recurrent vulvovaginal candidosis (RVVC), some patients with a long history of disease do not achieve complete resolution of symptoms following antimycotic treatment. It is thought that use of tight synthetic fabric underwear could be a significant factor in causing recurrence. We decided to compare underwear made of Dermasilk®, a pure fibroin fabric impregnated with a permanent antimicrobial protection, with a cotton placebo to see whether it could be a useful adjunctive tool in the management of RVVC. We recruited 96 women who had a long‐term history of RVVC and had not responded to oral antimycotics with complete satisfaction. The patients were randomly divided into two groups and instructed t...

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Antifungal activity of Coriandrum sativum essential oil, its mode of action against Candida species and potential synergism with amphotericin B

Tue, 26 Jul 2011 04:00:00 +0100

This study describes the antifungal activity of coriander essential oil on Candida spp., which could be useful in designing new formulations for candidosis treatment. (Source: Phytomedicine)

Reluctance of Caregivers to Perform Oral Care in Long-Stay Elderly Patients: The Three Interlocking Gears Grounded Theory of the Impediments

Fri, 15 Jul 2011 04:00:00 +0100

A clustered randomized controlled trial was previously carried out in long-stay facilities to assess the impact of oral care in the prevention of pneumonia and oral candidosis. However, despite an education program focusing on optimal oral care delivery practices and oral health knowledge improvement in the interventional arm, oral care seemed to be often underperformed or inadequately performed in both groups. Consequently, reluctance of caregivers to perform oral care emphasized the need for an analytical monitoring of the process of oral health care performed by registered nurses (RNs) and certified nursing assistants (CNAs). Therefore, we sought to develop a multivariate conceptual framework of the implicit impediments hampering the process of oral care by caregivers in the elderly. (S...

[Invasive fungal infections in oncology and haematology unit care: Review of literature and costs analysis].

Thu, 30 Jun 2011 23:00:00 +0100

Authors: Lecointre R, Bleyzac N Abstract Invasive fungal infections are an important cause of mortality in oncology and haematology unit care. Immunosuppression allows the occurrence of Candida or Aspergillus infectious disease. Treatment is based on antifungal agents (liposomal amphotericin B, azoles and caspofungin) administrated alone. The lack of study does not yet validate the combination of two drugs which are not recommended in medical practice. The aim of this pharmacoeconomics study is to assess different therapeutic strategies compared to standard treatment. Health care system point of view is used. Results show that liposomal amphotericin B is the reference standard drug during no documented infection in term of cost. But, voriconazole does not have significative cost va...

Spice oil cinnamaldehyde exhibits potent anticandidal activity against fluconazole resistant clinical isolates.

Thu, 23 Jun 2011 23:00:00 +0100

Authors: Shreaz S, Bhatia R, Khan N, Manzoor N, Muralidhar S, Khan LA Fluconazole resistance is becoming an important clinical concern. We studied the in vitro effects of cinnamaldehyde against 18 fluconazole-resistant Candida isolates. MIC(90) of cinnamaldehyde against different Candida isolates ranged 100-500μg/ml. Growth and sensitivity of the organisms were significantly affected by cinnamaldehyde at different concentrations. The rapid irreversible action of this compound on fungal cells suggested membrane-located targets for its action. Insight studies to mechanism suggested that cinnamaldehyde exerts its antifungal activity by targeting sterol biosynthesis and plasma membrane ATPase activity. Inhibition of H(+)-ATPase leads to intracellular acidification and cell death. Toxicity...

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Potential role of probiotic bacteria in the treatment and prevention of localised candidosis

Mon, 13 Jun 2011 23:00:00 +0100

SummaryThe extensive use of immunosuppressive therapies in recent years has increased the number of patients prone to or actually suffering from localised candidosis. As Candida species gain increasing resistance towards common antifungal drugs, new strategies are needed to prevent and treat infections caused by these pathogens. Probiotic bacteria have been in vogue in the past two decades. More and more dairy products containing such organisms offer promising potential beneficial effects on human health and well‐being. Because of the ability of probiotic bacteria to inhibit the growth of pathogens and to modulate human immune responses, these bacteria could provide new possibilities in antifungal therapy. We summarise the recent findings concerning the usefulness of probiotic treatment ...

Difference in virulence between fluconazole‐susceptible and fluconazole‐resistant Candida albicans in a mouse model

Sun, 22 May 2011 23:00:00 +0100

SummaryTwo Candida albicans isolates were collected from a HIV‐positive patient with recurrent oropharyngeal candidosis (OPC). One isolate was taken during the first episode of oral candidosis [fluconazole susceptible (FLU‐S), minimal inhibitory concentration (MIC) = 0.25 mg l−1] and the second after the patient developed refractory OPC and resistance to fluconazole (FLU‐R, MIC = 64 mg l−1). Both isolates were clonally identical. Different in vitro studies were carried out to assess putative virulence factors of both isolates. Gene expressions of efflux pumps and CSH1 were determined as well as adherence to human epithelial cells, determination of proteinase secretion and biofilm formation activity. Virulence was studied using a disseminated mouse model. All mice ...

The epidemiology, pathogenesis, and diagnosis of vulvovaginal candidosis: A mycological perspective.

Thu, 19 May 2011 23:00:00 +0100

This report reviews current knowledge about the available diagnostic methods and tests that accurately diagnose VVC, and highlights the importance of fungal culture. PMID: 21599498 [PubMed - as supplied by publisher] (Source: Critical Reviews in Microbiology)

ADH1 expression inversely correlates with CDR1 and CDR2 in Candida albicans from chronic oral candidosis in APECED (APS‐I) patients

Mon, 16 May 2011 23:00:00 +0100

AbstractExpression of the alcohol dehydrogenase gene ADH1, which converts ethanol into carcinogenic acetaldehyde, significantly inversely correlated with the expression of CDR1 and CDR2, genes linked to azole resistance in Candida albicans isolated from chronic oral candidosis in autoimmune polyendocrinopathy‐candidosis‐ectodermal dystrophy (APECED, APS‐I) patients. This is a novel link between candidal two‐carbon metabolism genes and azole resistance. (Source: FEMS Yeast Research)

Candida glabrata, Candida parapsilosis and Candida tropicalis: biology, epidemiology, pathogenicity and antifungal resistance

Thu, 12 May 2011 23:00:00 +0100

AbstractThe incidence of infections caused by Candida species (candidosis) has increased greatly over the past three decades, mainly due to the rise of the AIDS epidemic, an increasingly aged population, higher numbers of immunocompromised patients, and the more widespread use of indwelling medical devices. Candida albicans is the main cause of candidosis, however, non‐Candida albicans Candida species such as Candida glabrata, Candida tropicalis and Candida parapsilosis are now frequently identified as human pathogens. The apparent increased emergence of these species as human pathogens can be attributed to improved identification methods and also associated with the degree of diseases of the patients, the interventions that they were subjected and with the drugs used. Candida pathogenic...

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Recurrent vulvovaginal candidosis: focus on the vulva

Sat, 30 Apr 2011 23:00:00 +0100

SummaryRecurrent vulvovaginal candidosis is a frequent disease with a serious impact on women’s quality of life. Mostly, recurrences are caused by identical Candida strains suggesting C. albicans persistence in the female anogenital area. Objectives of the presented work were to identify the site of C. albicans persistence, to determine clinical symptoms and signs related to C. albicans positive vulvar cultures and to introduce a new therapeutic approach in women with RVVC. Women with an acute, culture‐confirmed episode of RVVC at time of visit were included in this prospective case series. Swabs were obtained from both vagina and inter‐labial sulcus. Women received a combined 20‐day regimen of 100 mg oral fluconazole and ciclopiroxolamin cream topically. Follow‐up visits were ...

Antifungal activity of the extract of Curcuma zedoaria (Christm.) Roscoe, Zingiberaceae, against yeasts of the genus Candida isolated from the oral cavity of patients infected with the human immunodeficiency virus

Fri, 01 Apr 2011 17:48:49 +0100

The objective of the present study was to investigate the possible antifungal action of the hydroalcoholic extract of Curcuma zedoaria (Christm.) Roscoe, Zingiberaceae, on yeasts in this population. Samples were collected from HIV-positive patients who attended the Laboratory for Teaching and Research in Clinical Analysis at the Universidade Estadual de Maringá for routine exams. The isolated yeasts were identified at the genus and species levels through classical methodology. Next, tests of microdilution in broth were carried out to determine the profile of susceptibility of these yeasts towards the hydroalcoholic extract of C. zedoaria, following methodology standardised by the CLSI (2002). A total of 53 yeasts were identified, 49 of them C. albicans, two C. tropicalis and two C. glabra...


Fri, 01 Apr 2011 08:00:04 +0100

Authors: Takakura S PMID: 21441708 [PubMed - in process] (Source: Nippon Ishinkin Gakk...)

Adherence and biofilm formation of non-Candida albicans Candida species.

Mon, 14 Mar 2011 00:00:00 +0100

Authors: Silva S, Negri M, Henriques M, Oliveira R, Williams DW, Azeredo J Most cases of candidosis have been attributed to Candida albicans, but recently non-C. albicans Candida species have been identified as frequent human pathogens. Candida pathogenicity has been attributed to several factors, including adhesion to medical devices and/or host cells, biofilm formation, and secretion of hydrolytic enzymes (proteases, phospholipases and haemolysins). Although 'new'Candida species are emerging, there is still a lack of information about their pathogenicity. This review discusses recent advances in our knowledge of Candida glabrata, Candida parapsilosis and Candida tropicalis virulence factors, specifically those of adhesion and biofilm formation, which are key components in Candida pat...

Group A streptococcal vaginitis: an unrecognized cause of vaginal symptoms in adult women

Fri, 18 Feb 2011 16:54:07 +0100

Conclusion  Vaginal infection with GAS in adult women is a clearly defined entity and should be considered a diagnosis when more common causes of vaginitis have been ruled out. Content Type Journal ArticlePages 1-4DOI 10.1007/s00404-011-1861-6Authors Hans Verstraelen, Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, De Pintelaan 185, B-9000 Ghent, BelgiumRita Verhelst, Department of Clinical Chemistry, Microbiology, and Immunology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, BelgiumMario Vaneechoutte, Department of Clinical Chemistry, Microbiology, and Immunology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, BelgiumMarleen Temmerman, Department of Obstetrics and Gynaecology,...

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The Role of the IL-12 Cytokine Family in Directing T-Cell Responses in Oral Candidosis

Mon, 14 Feb 2011 15:00:22 +0100

Candida albicans is an opportunistic fungal pathogen that normally exists as a harmless commensal in humans. In instances where host debilitation occurs, Candida can cause a range of clinical infections, and whilst these are primarily superficial, effecting mucosal membranes, systemic infections can develop in severely immunocompromised individuals. The mechanism of host immunity during commensal carriage of C. albicans has been intensively studied. In this paper, we present the most recent information concerning host recognition of C. albicans leading to cytokine production and the subsequent T-cell responses generated in response to C. albicans. Particular focus is given to the role of the IL-12 cytokine family including IL-12, IL-23, IL-27, and IL-35, in host immunity to Candida. CD4+ T...

Chemical composition and antifungal activity of the essential oils of Lavandula viridis L'Her.

Mon, 14 Feb 2011 00:00:00 +0100

Authors: Zuzarte M, Gonçalves MJ, Cavaleiro C, Canhoto J, Vale-Silva L, Silva MJ, Pinto E, Salgueiro L In the present work, we report for what we believe to be the first time the antifungal activity and mechanism of action of the essential oils of Lavandula viridis from Portugal. The essential oils were isolated by hydrodistillation and analysed by GC and GC/MS. The MIC and the minimal lethal concentration (MLC) of the essential oil and its major compounds were determined against several pathogenic fungi. The influence of subinhibitory concentrations of the essential oil on the dimorphic transition in Candida albicans was also studied, as well as propidium iodide and FUN-1 staining of Candida albicans cells by flow cytometry following short treatments with the essential oil. The oils ...

[Case Report] Heat, Oriental sore, and HIV

Fri, 11 Feb 2011 18:17:26 +0100

In January, 2009, a 34-year-old man presented to our hospital with a 6-month history of four, well-defined, non-tender, erythematous plaques of area 1–3 cm2 on his right hand (). Parasitological examination of a skin smear and biopsy sample confirmed a diagnosis of cutaneous leishmaniasis. Restriction-fragment length polymerisation PCR identified the causative species as Leishmania tropica. Our patient also had oral candidosis. He had no history of overseas travel, but his work as a truck driver took him all over India and he had had multiple sexual contacts with sex workers from many regions. (Source: LANCET)

Candida glabrata and Candida albicans co‐infection of an in vitro oral epithelium

Wed, 15 Dec 2010 00:00:00 +0100

Conclusions:  The results obtained demonstrating enhanced invasion and increased tissue damage caused by mixed C. glabrata and C. albicans infections have important clinical significance and highlight the need to identify Candida species involved in oral candidosis. (Source: Journal of Oral Pathology and Medicine)

Candida biofilms and oral candidosis: treatment and prevention

Wed, 08 Dec 2010 15:40:46 +0100

(Source: Periodontology 2000)

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Oral colonization by Candida species in AIDS pediatric patients

Mon, 29 Nov 2010 00:00:00 +0100

This study observed a low prevalence of Candida‐related oral lesions in these patients, which is compatible with the hypothesis that antiretroviral medicines may have contributed to reducing oral manifestations from Candida infection. The high prevalence of Candida colonization in HIV+/AIDS children with untreated dental caries reinforces the importance of oral health care in interdisciplinary health units that assist these patients. (Source: Oral Diseases)

Persistent Candida albicans colonization and molecular mechanisms of azole resistance in autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) patients

Tue, 09 Nov 2010 00:00:00 +0100

Conclusions Despite clinically successful treatment leading to relief of symptoms, colonization by C. albicans strains is persistent within APECED patients. Microevolution and point mutations occur within strains, leading to the development of azole-resistant isolates. (Source: Journal of Antimicrobial Chemotherapy)

Management of Recurrent Vulvo-Vaginal Candidosis as a Chronic Illness

Fri, 15 Oct 2010 23:00:00 +0100

Gynecol Obstet Invest 2010;70:306–321 (DOI:10.1159/000314022) (Source: Gynecologic and Obstetric Investigation)

Epidemiological survey of vulvovaginal candidosis in Sfax, Tunisia

Thu, 30 Sep 2010 23:00:00 +0100

Summary (Source: Mycoses)

The effect of antifungal treatment on the vaginal flora of women with vulvo-vaginal yeast infection with or without bacterial vaginosis

Tue, 28 Sep 2010 05:48:48 +0100

Abstract  Antibacterial therapy may enhance the risk of symptomatic vulvo-vaginal candidosis in susceptible women. We addressed the question whether oral antifungal treatment for vulvo-vaginal candidosis also influences the bacterial vaginal microflora. One hundred and forty-two patients with a culture-proven acute episode of recurrent vulvo-vaginal candidosis (RVC) were treated with fuconazole according to the ReCiDiF regimen (induction dose of 600 mg orally per week followed by 200 mg per week) or with a single dose of 200 mg pramiconazole, a new potent oral triazole. At inclusion, 1 week and 1 month after the end of antifungal treatment, the bacterial microflora was assessed by microscopy of vaginal fluid to detect lactobacillary grades and bacte...

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Preconceptional screening of sexually transmitted infections/diseases

Tue, 07 Sep 2010 17:21:51 +0100

Abstract  Hungarian Preconceptional Care includes the preconceptional screening of sexually transmitted infections/disorders (STD) and vaginal candidosis of potential mothers and pyospermia of potential fathers. The aim of this study was to evaluate the effect of this screening and treatment for the rate of preterm births. Clinical and subclinical vaginal candidiasis (asymptomatic candida colonisation), combination of STD and vaginal candidiasis, STD without vaginal candidiasis, finally women without STD and vaginal candidiasis as references were evaluated in 4,672 pregnant women. The association of STD in pregnant women with higher risk of preterm birth was confirmed. However, an association was also found between clinically diagnosed vaginal candidiasis, asymptomatic cand...

A new mucoadhesive dosage form for the management of oral lichen planus: formulation study and clinical study.

Mon, 09 Aug 2010 23:00:00 +0100

Authors: Cilurzo F, Gennari CG, Selmin F, Epstein JB, Gaeta GM, Colella G, Minghetti P The work aimed to study a new mucoadhesive prolonged release tablet containing 24 mug clobetasol-17 propionate (CP) suitable for the management of oral lichen planus. Low swellable dosage forms were designed by combining a mucoadhesive polymer, i.e. poly(sodium methacrylate, methylmethacrylate), with hydroxypropylmethylcellulose and MgCl(2). This formulation was selected to modify the tablet erosion rate in order to obtain a release of CP over a 6 h period. A double-blind controlled study was performed using three groups of patient (n=16) which received 3 applications-a-day over 4 weeks of the developed CP tablets (group CP-T), placebo tablets (group CP-P) or commercial CP ointment for cutaneous appl...

C. albicans activates cyclooxygenase but not its product prostaglandin E2 in HPV 16-stabilized cells

Sun, 11 Jul 2010 23:00:00 +0100

Conclusion: We found that Candida infection contributes only to cell cycle arrest and does not by itself contribute actively to the development of cancer, although it is associated with patients diagnosed as having cancer of the genital tract induced by HPV 16 virus. (Source: European Journal of Obstetrics, Gynecology, and Reproductive Biology)

The effect of brief exposure to sub-therapeutic concentrations of chlorhexidine gluconate on germ tube formation of oral Candida dubliniensis

Mon, 14 Jun 2010 23:00:00 +0100

Adherence of Candida has been implicated as the first step in the pathogenesis of oral candidosis, and germ tube formation, a contributory attribute. While chlorhexidine gluconate is by far the most common antiseptic mouthwash prescribed in dentistry, its intraoral concentration fluctuates considerably because of the dynamics of the oral cavity. Hence, the main objective of this study was to investigate the effect of brief exposure to three different sub-therapeutic concentrations of chlorhexidine gluconate on germ tube formation of Candida dubliniensis. Twelve oral isolates of C. dubliniensis were exposed to three different sub-therapeutic concentrations of 0.005%, 0.0025% and 0.00125% chlorhexidine gluconate for 30 min. The antiseptic was removed, and following subsequent incubation in a...

Genital candidosis in heterosexual couples

Tue, 08 Jun 2010 23:00:00 +0100

Conclusions Only in a few heterosexual couples a genetic similarity of Candida species recovered from both partners was found. RVVC women were more likely to have an asymptomatic candida negative sexual partner. This study suggests that male genitalia do not represent a relevant reservoir for RVVC; thus, the relevance of sexual transmission should not be emphasized. (Source: Journal of the European Academy of Dermatology and Venereology)

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Vaginal discharge: recommended management in general practice

Tue, 08 Jun 2010 23:00:00 +0100

Our Drug review of vaginitis considers the diagnosis and recommended management of bacterial vaginosis, vulvovaginal candidosis and trichomoniasis, followed by a review of prescription data and sources of further information. Copyright © 2010 Wiley Interface Ltd (Source: Prescriber)

Invasive fungal infections: epidemiology and analysis of antifungal prescriptions in onco-haematology

Tue, 08 Jun 2010 23:00:00 +0100

Conclusion: The incidence of IFI is low but the pharmacoeconomic impact is extremely high. Improved strategies are required to reduce the frequency and duration of empirical treatment without compromising beneficial outcome. (Source: Journal of Clinical Pharmacy and Therapeutics)

Determinants of gestational diabetes mellitus: A case control study in a district tertiary care hospital in south India

Tue, 20 Apr 2010 15:42:59 +0100

Conclusion: Early identification of women at risk of GDM and prompt treatment is recommended to prevent complications. (Source: International Journal of Diabetes in Developing Countries)