Thu, 23 Feb 2017 08:00:00 ESTSubject of the present invention is an in vitro method for therapy follow-up in septic patients wherein the concentration of mature ADM 1-52 and/or mature ADM 1-52-Gly in a sample of bodily fluid of said septic patient is determined using an assay comprising two binders that bind to two different regions within the region of mature adrenomedullin and/or adrenomedullin-Gly that is aminoacid 21-52-amid SEQ. ID No. 1 or aminoacid 21-52-Gly SEQ ID No. 2 wherein each of said regions comprises at least 4 or 5 amino acids. Subject of the present invention are further assays and calibration methods.
Thu, 23 Feb 2017 08:00:00 ESTThe present application provides stable peptide-based IL-17F capture agents and methods of use as detection agents. The application further provides methods of manufacturing IL-17F capture agents.
Thu, 23 Feb 2017 08:00:00 ESTThe present invention pertains to a method of detection, by mass spectrometry, of at least one marker of at least one mechanism of resistance to at least one antimicrobial, resistance of at least one microorganism contained in a sample, characterised in that the antimicrobial is a cephalosporin, and said resistance markers are proteins or peptides. Preferably, said proteins or peptides are proteins from said microorganism.
Thu, 23 Feb 2017 08:00:00 ESTMethods are provided for quantifying the Androgen receptor protein (AR) protein directly in biological samples that have been fixed in formalin, using Selected Reaction Monitoring (SRM)/Multiple Reaction Monitoring (MRM) mass spectrometry. The biological samples are chemically preserved and fixed and can be, for example, tissues treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks. A protein sample is prepared from said biological sample using, for example, the Liquid Tissue protocol and the AR protein is quantitated in the Liquid Tissue sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described.
Thu, 23 Feb 2017 08:00:00 ESTPeptides from the tyrosine-protein kinase receptor UFO protein (AXL) are provided that are particularly advantageous for quantifying the AXL protein directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM)/Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed and include formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks. A protein digest is prepared from the biological sample and the AXL protein is quantitated in the Liquid Tissue sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described.
Thu, 23 Feb 2017 08:00:00 ESTReagents and methods for the digital analysis of proteins or peptides are provided. Specifically provided herein are proteins for identifying the N-terminal amino acid or N-terminal phosphorylated amino acid of a polypeptide. Also, an enzyme for use in the cleavage step of the Edman degradation reaction and a method for using this enzyme are described.
Thu, 23 Feb 2017 08:00:00 ESTA pregnancy monitoring device and method improve detection in the first trimester of the occurrence of ectopic pregnancy and the occurrence of multiple foetuses. The device includes a sample absorbing member and an analyser to analyse a plurality of sequential a biological liquid samples and computing means to determine an ovulation date and deviations in the measured quantity of hCG from predetermined values which are indicators of ectopic pregnancy or multiple pregnancy. The device and method enables early detection of ectopic pregnancy or multiple pregnancy.
Thu, 23 Feb 2017 08:00:00 ESTA biological process indicator is provided for validating a treatment process in which the amount or activity of a contaminant in a sample is reduced. The indicator comprises a thermostable kinase covalently linked to a biological component, with the proviso that the biological component is not an antibody. Methods of preparing the indicator, and methods of using the indicator, are also provided.
Thu, 23 Feb 2017 08:00:00 ESTThe present disclosure provides detection methods employing HCV core lipid binding domain and DNA binding domain monoclonal antibodies or antibody fragments. In certain embodiments, the lipid binding domain monoclonal antibody or antibody fragment recognizes an epitope in amino acids 141 to 161 of HCV core protein and the DNA binding domain antibody or antibody fragment recognizes an epitope in amino acids 95-123 (e.g., in amino acids 99-117) of HCV core protein.
Thu, 23 Feb 2017 08:00:00 ESTThe present disclosure provides detection methods employing HCV core lipid binding domain and DNA binding domain monoclonal antibodies. In certain embodiments, the lipid binding domain monoclonal antibody recognizes an epitope in amino acids 141 to 161 of HCV core protein.
Thu, 23 Feb 2017 08:00:00 ESTThe present invention relates to polynucleotides enabling the rapid, simple and specific detection of Group B Streptococcus highly-virulent ST-17 clones. The present invention also relates to the polypeptides encoded by said polynucleotides, as well as to antibodies directed or raised against said polypeptides.The present invention also relates to kits and methods for the specific detection of Group B Streptococcus highly-virulent ST-17 clones, using the polynucleotides, the polypeptides or the antibodies according to the invention.
Thu, 23 Feb 2017 08:00:00 ESTSystems and methods are provided for microbial identification using cleavable tags. Control information is sent to a mass spectrometer to select a peptide labeled with a first tag of a known microbe, fragment the labeled peptide of the known microbe, and monitor for an intensity of the first tag in an MRM method using a processor. An ion source provides a beam of ions from a sample that includes peptides labeled with the first tag. The first tag binds to a peptide of a known microbe and is cleaved from the peptide of the known microbe during mass spectrometry. The mass spectrometer receives the beam of ions and is adapted to perform the MRM method on the beam of ions. If the intensity of the first tag received from the mass spectrometer exceeds a threshold value, the known microbe is identified in the sample using the processor.
Thu, 23 Feb 2017 08:00:00 ESTThis disclosure relates to the field of diagnostic methods. A new marker antibody for rheumatoid arthritis, as well as a new method for detecting rheumatoid arthritis, is disclosed herein. Also provided herein are means and methods for predicting whether a subject will develop rheumatoid arthritis. This disclosure is based on the discovery that patients with rheumatoid arthritis have antibodies in their circulation that react specifically with citrullinated antibodies. In one aspect, the disclosure, therefore, relates to an antibody comprising a citrulline residue. In another aspect, the disclosure provides a method for the detection of antibodies specific for rheumatoid arthritis in a sample from a subject, wherein the sample is contacted with a citrullinated antibody and wherein it is determined whether the sample comprises antibodies specifically reactive with the citrullinated antibody.
Thu, 23 Feb 2017 08:00:00 ESTThe invention provides new methods of isolating target cells using a solid phase, the solid phase comprising a ligand L wherein the ligand L is capable of specifically binding a ligand binding partner LB, the ligand binding partner LB being present in a receptor molecule binding reagent or a multimerization reagent used for isolating target cells. The invention also provides corresponding new arrangements and devices for isolating a target cell from a sample.
Thu, 23 Feb 2017 08:00:00 ESTThe present application relates to a biosensor that employs an acoustic cavity to store mechanical energy. In particular examples, the biosensor includes an electrode region and one or more reflector regions to form the acoustic cavity, as well as a functionalized active area disposed in proximity to the cavity. Methods of making and using such biosensors are also described herein.
Thu, 23 Feb 2017 08:00:00 ESTA reference control composition for use therein and methods for making the same, including a plurality of spermatozoa from a non-human source for simulating human spermatozoa and an effective amount of an agent for maintaining the plurality of spermatozoa in a substantially aggregate-free condition.
Thu, 23 Feb 2017 08:00:00 ESTThe present invention relates to an immune hepatotoxicity screening method using hepatocytes derived from human stem cells. After hepatocytes differentiated from human stem cells and human hepatocytes are treated with ethanol, CCl4, and acetaminophen to induce immune hepatotoxicity, a hepatocellular immunotoxic material assay system is constructed in order to verify cytokines, chemokines, and lipid mediators, which are mediators secreted from the hepatocytes, and an immunotoxic material can be confirmed in the cells having the induced hepatotoxicity by using the system. Therefore, the immune hepatotoxicity screening method using hepatocytes derived from human stem cells can be favorably used.
Thu, 23 Feb 2017 08:00:00 ESTIn the present invention, for test cells which are either stem cells whose state of differentiation is unknown or cells obtained from stem cells by differentiation induction, an LC-MS or GC-MS analysis is performed on culture supernatants collected from a culture dish of the test cells and a culture dish of control cells whose state of differentiation is known, and the state of differentiation of the test cells is assessed based on the amount, determined as a result of the aforementioned analysis, of at least one compound selected from the group of putrescine, kynurenine, cystathionine, ascorbic acid, riboflavin, pyruvic acid, serine, cysteine, threonic acid, citric acid, and orotic acid in both the culture supernatant of the test cells and the culture supernatant of the control cells.
Thu, 23 Feb 2017 08:00:00 ESTThe invention relates to novel cells and cell lines, and methods for making and using them.
Thu, 23 Feb 2017 08:00:00 ESTA microfluidic sensor, such as an electrochemical blood test strip, with more accurate measurement comprises a plurality of channels through which a fluid to be tested flows via a capillary action. One or more electrodes are located under the channels. As the fluid flows over the electrodes in the channels, the impedance between the electrodes may be measured to determine fluid properties. In order to increase the accuracy of the measurements, the electrode deposition may be configured to be less than 10 μm in thickness via a printing process with high process consistency, thereby reducing the disruption of the electrode deposition on the fluid flow.
Thu, 23 Feb 2017 08:00:00 ESTA portable bioreactor is provided for the in situ study of mesophilic and thermophilic corrosion inducing microbial biofilms on metal surfaces used in any industry, such as oil, chemical, petrochemical, oil refining, food, metallurgical, paper. The bioreactor's configuration is a “batch” type for turbulent and piston-driven laminar flow, operating by cycles, with the continuous circulation of fluid. A culture medium, or industrial operation or production fluids containing microbiota is introduced into the load of the receiving body, thus the bioreactor is single phase. The bioreactor comprises a support section for corrosimetric test coupons that are in touch with the fluid under dynamic conditions, such as found in the fluid carrying pipelines, naturally promoting the formation of the microbial biofilm. The coupons are removed for analysis to follow the kinetics of microbial biofilm development. The operating conditions comprise a section having sufficient turbulence for the fluid to homogenize and maintain a temperature of 20 to 80° C. necessary for the mesophilic or thermophilic microorganism's growth. Salinity can be in the range 2 to 200 ppm (NaCl) and the pH range from 2 to 10; the bioreactor's operating conditions conform to the physicochemical characteristics of the fluid from the industry to be assessed.
Thu, 23 Feb 2017 08:00:00 ESTDisclosed herein are embodiments of compounds, conjugates, and devices, such as columns comprising such compounds and/or conjugates, that can be used to identify, separate, and quantify post-translationally modified analytes. The disclosed compounds and conjugates can be used to discriminate between analytes, such as peptides, having different post-translation modifications, such as methylations, phosphorylations, acetylations, citrullinations, hydroxylations, nitrosylations, ADP-ribosylations, glycosylations, propionylations, butyrylations, crotonylations, 2-hydroxyisobutyrylations, malonylations, succinylations, formylations, ubiquitinations, neddylations, proline cis-trans isomerizations. In particular disclosed embodiments, the compounds and conjugates can be used to separate peptides having different degrees of methylation.
Thu, 23 Feb 2017 08:00:00 ESTThe method for detecting an analyte of the present invention comprises the steps of: coating an electrode with at least one of an aniline polymer formed by substituting hydrogen of a monomer with a boronic acid, followed by electropolymerization, a thiopene polymer, a pyrrole polymer, a carbon nanotube and graphene; exposing the electrode to an analyte that is present in a solution or air; and measuring impedance generated from the electrode exposed to the analyte. The present invention forms the aniline polymer by electropolymerization of an aniline monomer which is formed by substituting hydrogen with a boronic acid in the step of coating the electrode, and utilizes the electrode as a biosensor after coating the electrode with the aniline polymer. Further, in the step of measuring impedance, the presence/absence of an analyte is detected from a change in conductivity.
Thu, 23 Feb 2017 08:00:00 ESTAn impedance spectroscopy biosensor is provided that is fabricated on a stretchable substrate. The stretchable substrate is integrated with an impedance biosensor that undergoes cyclic strain without cracking.
Thu, 23 Feb 2017 08:00:00 ESTA method of exchanging fluids with suspended particles includes providing a microfluidic device with a first inlet channel operatively coupled to a source of particles and a second inlet channel operatively coupled to an exchange fluid. A transfer channel is connected at a proximal end to the first inlet channel and the second inlet channel. First and second outlet channels are connected to a distal end of the transfer channel. The source of particles is flowed at a first flow rate into the first inlet channel while the exchange fluid is flowed at a second flow rate into the second inlet channel wherein the ratio of the second flow rate to the first flow rate is at least 1.5. Particles are collected in one of the first and second outlet channels while fluid substantially free of particles is collected in the other of the first and second outlet channels.
Thu, 23 Feb 2017 08:00:00 ESTAn apparatus and method of identifying objects includes: a microfluidic chip in which are disposed a plurality of channels, the microfluidic chip including: a main fluid channel into which a sample fluid mixture of objects to be identified is introduced; a plurality of sheath fluid channels into which sheath fluids are introduced, the sheath fluids which orient the objects in the main fluid channel in a predetermined direction while still maintaining laminar flow in the main fluid channel; an interrogation apparatus which detects and interrogates the oriented objects in the main fluid channel; and a focused energy apparatus which performs an action on the objects.
Thu, 23 Feb 2017 08:00:00 ESTThe present invention refers to a formal-in-free fixative composition, suitable for the fixation of cells in particular in liquid samples, the use of said fixative for the treatment of biological samples, a method for the treatment of cell-comprising liquid samples, a kit comprising said fixative and a method for diagnosis of cell-comprising biological material samples.
Thu, 23 Feb 2017 08:00:00 ESTProvided herein are methods for determining the presence or absence of an enteroviruses and parechoviruses in a biological sample. The methods involve identifying the presence or absence of a target nucleic acids from the viruses using direct amplification from a biological sample without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids. Also provided are methods of diagnosis using the methods provided and compositions and kits for the practice of the methods.
Thu, 23 Feb 2017 08:00:00 ESTThe present disclosure provides genetically engineered cell lines comprising chromosomally integrated synthetic sequences having predetermined epigenetic modifications, wherein a predetermined epigenetic modification is correlated with a known diagnosis, prognosis or level of sensitivity to a disease treatment. Also provided are kits comprising said epigenetically modified synthetic nucleic acids or cells comprising said epigenetically modified synthetic nucleic acids that can be used as reference standards for predicting responsiveness to therapeutic treatments, diagnosing diseases, or predicting disease prognosis.
Thu, 23 Feb 2017 08:00:00 ESTThe present inventions relates to methods and assays to predict the response of an individual to a psychiatric treatment and to a method to improve medical treatment of a disorder, which is responsive to treatment with a psychiatric treatment.
Thu, 23 Feb 2017 08:00:00 ESTThe present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
Thu, 23 Feb 2017 08:00:00 ESTA composition is provided comprising a negatively charged group, an oligonucleotide sequence and at least none or one nuclease-resistant linkage group to form a chemically-enhanced primer. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the primer as well as a method of preparing DNA for sequencing and a method of sequencing DNA and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
Thu, 23 Feb 2017 08:00:00 ESTThe present invention provides methods, kits, and compositions for producing single-stranded circular DNA by PCR. In particular, hairpin primers are provided, and methods of use thereof to produce single-stranded circular DNA molecules.
Thu, 23 Feb 2017 08:00:00 ESTProcess and apparatus for the optimization of DNA detection and comprising: charging a plurality of reaction vessels with reagents and primers suspected of being suitable for the particular sample, in various quantities; placing in each reaction vessel a sample of the target DNA; subjecting each vessel concurrently to PCR; simultaneously observing optically the whole PCR process in each reaction vessel.
Thu, 23 Feb 2017 08:00:00 ESTA fluidic device is disclosed for processing a biological sample in order to extract nucleic acids contained in said sample and for subsequently amplifying said extracted nucleic acids, said device including a processed sample storage archive area 10 comprising an absorbent solid substrate 14 treated with at least one nucleic acid stabilising reagent or reagent mix, said substrate allowing the generally dry and stabilised storage of said extracted and/or amplified nucleic acids, for example for long term storage of biological samples recovered forensically.
Thu, 23 Feb 2017 08:00:00 ESTThe present invention relates to a simple and highly efficient method for the isolation of nucleic acids from a sample, methods of lysing samples from which the nucleic should be obtained, and materials that can be used in such methods.
Thu, 23 Feb 2017 08:00:00 ESTA sample preparation device comprising a first chamber containing a first movable piston separating a first volume upstream and a second volume downstream the first piston, a second chamber containing a second movable piston separating a third volume upstream and a fourth volume downstream the second piston, and an inlet to the first volume and an outlet from the first volume. The first volume is connected with the third volume by a first communication path and the second volume is connected with the fourth volume by a second communication path.
Thu, 23 Feb 2017 08:00:00 ESTThe invention relates to a method for identifying unknown microbes in a sample, wherein a mass spectrometric determination termination down to the taxonomic level of the genus or species is supplemented by a detailed determination of a lower taxonomic level or variety by means of infrared spectrometry, using restricted reference libraries of infrared spectra. These libraries can be genus-specific, containing only infrared spectra of microbes of one genus, or species-specific, containing only infrared spectra of microbes of one species. In so doing, a robust mass spectrometric identification of the species of unknown microbes is advantageously supplemented with a detailed analysis of the subspecies and varieties by means of infrared spectrometry, primarily in order to identify medically important varieties such as pathovars like EHEC and EPEC, and antibiotic-resistant microbes like MRSA.
Thu, 23 Feb 2017 08:00:00 ESTAn enzymatic method is provided for restructuring an affinity ligand bound heterogenous glycoform antibody sample to a substantially homogenous single desired glycoform antibody sample for therapeutic uses and kits for performing the methods. A method for enzymatically altering the Fc region of an affinity ligand bound antibody from a heterogenous glycoform to a substantially homogenous single glycoform comprises: contacting the affinity ligand bound heterogeneous glycoform antibody with a reaction buffer designed for a particular glycoform modification for a time sufficient and under conditions to modify the glycoform of the Fc region to a substantially homogeneous single form; optionally adding one or more nucleotide sugars and/or cofactors; and releasing the substantially homogeneous single glycoform antibody sample from said affinity ligand. The invention also encompasses biopharmaceuticals comprising single glycoform mAbs and polyclonal antibodies enzymatically produced for the treatment of cancers and immune disorders as well as compositions comprising the single glycoform antibodies as a biopharmaceutical.
Thu, 23 Feb 2017 08:00:00 ESTThe invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable Circumstances Processes for improved production of isolated mammalian, particularly human, proteins are provided.
Thu, 23 Feb 2017 08:00:00 ESTThe present invention provides improved DNA polymerases, in particular, type A DNA polymerases, that may be better suited for applications in recombinant DNA technologies. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.
Thu, 23 Feb 2017 08:00:00 ESTDisclosed are methods for ergothioneine biosynthesis. More particularly, the present disclosure relates to methods for microbial ergothioneine biosynthesis. The present disclosure relates generally to engineered host cells and methods for producing ergothioneine. More particularly, the present disclosure relates to an engineered host cell and methods for microbial ergothioneine biosynthesis using the engineered host cell.
Thu, 23 Feb 2017 08:00:00 ESTIt has been found, surprisingly, that the Corynebacterium humireducens strain comprises a very effective glycine cleavage system.
Thu, 23 Feb 2017 08:00:00 ESTSurprisingly, it has been found that alkaliphilic bacteria of the genus Corynebacterium are naturally suited to produce L-amino acids.
Thu, 23 Feb 2017 08:00:00 ESTThe present disclosure relates to methods and systems for injecting a gas (e.g., air) into a grain oil recovered from a grain process so as to remove an amount of one or more materials (e.g., moisture, unsaponifiables, insolubles, free fatty acids, and the like) from the grain oil.
Thu, 23 Feb 2017 08:00:00 ESTProvided are: a novel yeast having an ability to efficiently produce ethanol from glucose and xylose in a short time in the coexistence of the glucose and the xylose; and a method for producing ethanol using the novel yeast. A yeast, which was designated as Candida intermedia 4-6-4T2 and was deposited as FERN BP-11509.
Thu, 23 Feb 2017 08:00:00 ESTA liquid fuel production method of the present invention includes: a saccharification step in which a biomass is saccharified; a methane fermentation step in which a saccharified liquid acquired in the saccharification step undergoes methane fermentation; and a biogas to liquid (BTL) step in which a liquid fuel is generated from a biogas acquired in the methane fermentation step.
Thu, 23 Feb 2017 08:00:00 ESTAn object is to provide a thermotolerant yeast capable of assimilating xylose to produce ethanol or a mutant strain thereof and a method for producing ethanol by assimilation of xylose. There is used a yeast, Kluyveromyces marxianus strain No. 21 (accession number: NITE BP-01739), or a mutant strain thereof that has xylose assimilation ability and ethanol production ability when cultured at 30° C. under aerobic conditions using a culture medium containing xylose as a sugar source. Also performed is a method for producing ethanol, comprising culturing the yeast or the mutant strain thereof under aerobic conditions using a culture medium containing xylose as a sugar source.
Thu, 23 Feb 2017 08:00:00 ESTMethod and apparatus for the production of hydrogen and other gaseous or liquid substances (such as 2,3-butanediol) formed with the help of microbes in conditions where the normal microbial metabolism (catabolism and anabolism) has been restricted by pH or temperature, for example. Then carbon is not liberated into gaseous phase as fast as in more common microbial reactions. Carrier gas directed into organic waste or other biomass is helping in liberating molecular hydrogen into gaseous phase with the aid of microbial enzymes or electric phenomena at the same time when new hydrogen is binding into the biomass from water. Removed gases or combustion gases from the incineration plants can be directed back into bioprocess in some process alternatives, together with lowering total carbon emission by these means. The production plant is planned in such a way that it can be situated in the midst of inhabitation.
Thu, 23 Feb 2017 08:00:00 ESTThe present invention provides methods for improving microbial tolerance to alpha olefin compounds, host cells having increased tolerance to such compounds, and method of using the host cells to produce alpha olefin compounds.