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SYSTEM AND METHOD FOR FETAL AND MATERNAL RED BLOOD CELL COUNTING

Thu, 06 Apr 2017 08:00:00 EDT

A system for counting fetal and maternal red blood cells (RBCs) including a microscope and image capturing device to capture at least one image from a slide holding the fetal and maternal RBCs; a computer readable medium for storing the at least one image; a processor for executing computer readable instructions stored on a computer readable medium; wherein the computer executable instructions include instructions for: indentifying red blood cells from the at least one image; distinguishing between fetal and maternal red blood cells; and counting the fetal and maternal red blood cells.



METHOD, APPARATUS AND SYSTEM FOR DETECTING AND DETERMINING COMPROMISED REAGENT PADS BY QUANTIFYING COLOR CHANGES INDUCED BY EXPOSURE TO A HOSTILE ENVIRONMENT

Thu, 06 Apr 2017 08:00:00 EDT

A reagent test paddle includes a contamination detection medium, a reference color bar, at least one chemical test medium, and a unique identifier. The contamination detection medium includes a reagent that changes color in the presence or when exposed to a hostile or inhospitable environment. Each chemical test medium includes a regent that is responsive to a respective analyte in a biological sample. The reference color bar includes reference color samples of different colors. The unique identifier, like a serial number, identifies the particular paddle and its chemical test medium so it can be uniquely and anonymously associated with a user. A method includes capturing and interpreting digital images of a biologically unexposed and subsequently exposed reagent test paddle at various delay times within an automatically calibrated environment; locating the paddle in a plurality of digital images, extracting the reference color bar and locating the contamination detection medium and chemical test medium in each digital image. Color changes of the chemical test medium and contamination medium are detected at various delay times after sample exposure. To determine validity of test results, the method further compares the detected colors of the contamination detection medium with predetermined colors expected for no contamination and contamination.



Method of Receiving and Handling a Plurality of Clinical Samples for Reporting a Sum of Diagnostic Results for Each Sample

Thu, 06 Apr 2017 08:00:00 EDT

A method is provided for receiving and handling a plurality of clinical samples and managing information associated therewith for generating and reporting any of a plurality of different diagnostic results from each sample in a timely manner, particularly within about thirty (30) hours. Methods described comprise, for example, receiving a plurality of single gynecological swab samples, each having identity and test requisition information associated therewith, wherein the test requisition information indicates a test for at least one causative agent, from a choice of a plurality of agents (for example, between about 5 and about 25 different microbiological agents) and managing information associated therewith for generating and reporting any of a plurality of different diagnostic results for each sample.



AUTOMATED SELECTION OF MICROORGANISMS AND IDENTIFICATION USING MALDI

Thu, 06 Apr 2017 08:00:00 EDT

A method and apparatus for locating and selecting a colony of microorganisms on a culture dish and identifying microorganisms in said selected colony using MALDI. The method comprises the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of said selected colony of microorganisms; depositing at least some of said sample of said selected colony of microorganisms on a target plate; and transferring said target plate with said sample in an apparatus for performing MALDI for identification of said sample of said selected colony of microorganisms. A sample of a colony of microorganisms is automatically deposited on a depositing spot such that the sample covers at most approximately half of said one of the depositing spots of the target plate. A suspension of a sample of microorganisms is automatically prepared by automatically picking the sample with a picking tool and submerging the picking tool with said sample in a suspension, after which the picking tool is vibrated in vertical sense only to release the sample from the picking tool.



CARTRIDGE DEVICE FOR A MEASURING SYSTEM FOR MEASURING VISCOELASTIC CHARACTERISTICS OF A SAMPLE LIQUID, A CORRESPONDING MEASURING SYSTEM, AND A CORRESPONDING METHOD

Thu, 06 Apr 2017 08:00:00 EDT

The present invention is directed to a cartridge device for a measuring system for measuring viscoelastic characteristics of a sample liquid. In particular a blood sample, comprising a cartridge body having at least one measurement cavity formed therein and having at least one probe element arranged in said at least one measurement cavity for performing a test on said sample liquid; and a cover being attachable on said cartridge body; wherein said cover covers at least partially said at least one measurement cavity and forms a retaining element for retaining said probe element in a predetermined position within said at least one measurement cavity. The invention is directed to a measurement system and a method for measuring viscoelastic characteristics of a sample liquid.



GLUTAMINYL CYCLASE AS A DIAGNOSTIC/PROGNOSTIC INDICATOR FOR NEURODEGENERATIVE DISEASES

Thu, 06 Apr 2017 08:00:00 EDT

A method for predicting, diagnosing and prognosticating a neurodegenerative disease, such as Alzheimer's disease (AD), Mild Cognitive Impairment (MCI) and neurodegeneration in Down's syndrome (NDS) using glutaminyl cyclase (QC) as a diagnostic/prognostic indicator. The use of antibodies binding to QC and kits for performing said diagnostic method are also provided.



METHOD AND DEVICE FOR STABILIZING OF PROTEINS

Thu, 06 Apr 2017 08:00:00 EDT

A method relating to preparation of a biological sample for analysis by flow cytometry including contacting a drawn blood sample with a protective agent that is substantially free of formaldehyde and analyzing the white blood cells of the contacted sample for protein epitopes after at least about twenty-four hours after contacting.



METHODS AND REAGENTS FOR ANALYZING PROTEIN-PROTEIN INTERFACES

Thu, 06 Apr 2017 08:00:00 EDT

The present disclosure provides methods and reagents useful for analyzing protein-protein interfaces such as interfaces between a presenter protein (e.g., a member of the FKBP family, a member of the cyclophilin family, or PIN1) and a target protein. In some embodiments, the target and/or presenter proteins are intracellular proteins. In some embodiments, the target and/or presenter proteins are mammalian proteins.



COVALENT TETHERING OF FUNCTIONAL GROUPS TO PROTEINS AND SUBSTRATES THEREFOR

Thu, 06 Apr 2017 08:00:00 EDT

A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.



BIOMARKERS AND METHODS TO DISTINGUISH OVARIAN CANCER FROM BENIGN TUMORS

Thu, 06 Apr 2017 08:00:00 EDT

Methods for detecting and measuring metabolic changes useful in the detection of cancer, and in differentiating between ovarian cancer and benign ovarian tumor are described, as well as the unexpected and valuable combination of detecting both lipidomic and aqueous metabolites. Two independent LC-MS-based metabolomics platforms, including a global lipidomics approach, were used to screen for differentially abundant plasma metabolites between cases with serous ovarian carcinoma and controls with benign serous ovarian tumor. The identified biomarkers can be used to distinguish between ovarian cancer and benign tumors. Standards and kits for use with the methods for detecting cancer are also provided.



ALPHA METHYLACYL A COENZYME RACEMASE DETECTION

Thu, 06 Apr 2017 08:00:00 EDT

A method of detecting the presence or absence of alpha methylacyl A coenzyme racemase (AMACR) is provided. In one embodiment of the method, a fluid including a secretion chosen from at least one of a prostate secretion or a secretion from an accessory glad or a cellular component of the fluid is obtained from a subject. The fluid is contacted with a reagent for detecting AMACR under conditions such that the reagent detects AMACR in the semen. The level of AMACR is determined such as by comparing it to a standard curve. The fluid can be semen or a prostate secretion or another fluid. A kit that can be used with the method is also provided.



COMPOSITIONS AND METHODS FOR IMPROVED CELL-BASED BOTULINUM NEUROTOXIN ASSAYS

Thu, 06 Apr 2017 08:00:00 EDT

Methods and compositions are provided where a transfected cell that produces a hybrid protein with a reporter-containing portion and a botulinum toxin cleavage site is contacted with a botulinum toxin in media having a reduced sodium concentration, thereby increasing the sensitivity. Such media can also be used in combination with elevated cell culture temperatures. Kits that include such media and a botulinum toxin are also described.



POLYMERSOME ENCAPSULATION OF HYDROPHOBIC FLUORESCENT POLYMERS

Thu, 06 Apr 2017 08:00:00 EDT

Described herein are aqueous soluble polymersomes that encapsulate one or more hydrophobic fluorescent polymers and methods of their preparation and use.



ASSAY PLATE AND USES THEREOF

Thu, 06 Apr 2017 08:00:00 EDT

Provided herein are assay plates and uses thereof. In particular, provided herein are assay plates comprising optofluidic channels and microposts for use in performing biological and chemical assays and detecting assay results. The described plates provide reduced assay time and sample volume, and increased sensitivity and specificity in biological and chemical assays.



Portable Instrument for In Vitro Detection and Quantification of Biomarkers

Thu, 06 Apr 2017 08:00:00 EDT

A portable assay instrument for rapid detection and quantification of biomarkers is described. The instrument may include components to automate steps of an assay. Assay-specific agitation during an incubation phase in which capture binding agents, detection binding agents, and conjugates are present in sample wells can appreciably increase the speed of the assay and improve assay sensitivity. The instrument may further include an optical detector and processor for automated probing of the sample wells and computation of biomarker concentration.



SYSTEM AND METHOD FOR DETECTING TARGET SUBSTANCES

Thu, 06 Apr 2017 08:00:00 EDT

A method includes capturing a signal of a detection substrate exposed to a sample containing a target substance; determining that the detection substrate is in a testable state; and generating an assessment of the presence of the target substance in the sample.



CHARACTERIZING LIQUIDS USING MAGNETIC DISCS

Thu, 06 Apr 2017 08:00:00 EDT

The present disclosure is directed towards characterizing liquids through the use of magnetic discs that rotate in response to dynamic magnetic fields. In some embodiments, a light beam is transmitted into the liquid while the magnetic discs rotate, and one or more parameters a light beam signal associated with the transmitted light beam are identified. Various characteristics of the liquid may be detected based on the one or more parameters of the light beam signal.



CELL BASED ASSAY TO MEASURE THE T-CELL STIMULATING CAPACITY OF ANTI-LAG3 ANTIBODIES AND OTHER AGENTS

Thu, 06 Apr 2017 08:00:00 EDT

The present invention includes a human LAG3 functional assay using a Jurkat T-cell lymphoma cell line engineered to overexpress LAG3 at an optimal level relative to CD3. The assay is useful, for example, for determining the immunostimulatory properties of LAG3 modulators (e.g., inhibitors or activators). The optimized LAG3/CD3 ratio ensures expression of optimal receptor components on the T-cell and, thus, superior assay sensitivity. Immunostimulation of the T-cells can be measured, for example, by following cytokine (e.g., IL-2) production. The optimized T-cell line forms part of the present invention along with compositions generated with use of the assay.



DUAL DIELECTROPHERETIC MEMBRANE FOR MONITORING CELL MIGRATION

Thu, 06 Apr 2017 08:00:00 EDT

A dual dielectropheretic article for monitoring cell migration includes: a membrane to selectively migrate a plurality of cells across the membrane, the membrane including: a first surface to receive the cells; a second surface opposed to the first surface; and a plurality of communication paths disposed in the membrane to provide the selective migration of the cells across the membrane from the first surface to the second surface; a first electrode disposed on the first surface to: provide an electric field for dielectrophoresis of the cells at the first surface; and provide a first potential for monitoring an impedance at the first surface; and a third electrode disposed on the second surface to: provide an electric field for dielectrophoresis of the cells at the second surface; and provide a third potential for monitoring an impedance at the second surface.



CHEMICAL DETECTION DEVICE HAVING MULTIPLE FLOW CHANNELS

Thu, 06 Apr 2017 08:00:00 EDT

The described embodiments may provide a chemical detection circuit that may comprise a plurality of first output circuits at a first side and a plurality of second output circuits at a second side of the chemical detection circuit. The chemical detection circuit may further comprise a plurality of tiles of pixels each placed between respective pairs of first and second output circuits. Each tile may include four quadrants of pixels. Each quadrant may have columns with designated first columns interleaved with second columns. Each first column may be coupled to a respective first output circuit in first and second quadrants, and to a respective second output circuit in third and fourth quadrants. Each second column may be coupled to a respective second output circuit in first and second quadrants, and to a respective first output circuit in third and fourth quadrants.



SPECIMEN PROCESSING SYSTEMS, PIPETTE ASSEMBLIES AND METHODS FOR PREPARING REAGENTS

Thu, 06 Apr 2017 08:00:00 EDT

Systems and methods that enable automated processing of specimens carried on microscope slides are described herein. Aspects of the technology are directed, for example, to automated slide processing apparatuses capable of dispensing liquids onto microscope slides. Additional aspects of the technology are directed to methods of replacing a reagent pipette in automated slide processing apparatuses. The apparatus can include, for example, a reagent pipette assembly including a reagent pipette moveable between at least one loading position for obtaining reagent from a reagent container at a filling station and at least one dispense position. The apparatus can also include a retainer for releasably securing the reagent pipette. In some embodiments, the reagent pipette assembly includes a locking mechanism for transitioning the retainer from an open configuration for receiving a pipette and a closed configuration for securing a pipette, in e.g., an aligned position within the retainer.



Micro-Sampling for Aquatic Chemical Analysis

Thu, 06 Apr 2017 08:00:00 EDT

The current invention describes in vivo and vitro (cultured) sampling technologies that allow direct temporal and spatial sampling from living ecosystems such as those associated with marine ecology. The optional use of parallel sampling methods, observatory design, provides for the ability to measure the response of individual organisms to a variety of both biotic and abiotic stresses. Sampling in small volumes and close proximity to living organisms has allowed direct measurement of various invertebrate and other aquatic species in marine ecosystems. These sampling techniques are intended to apply to any liquid based ecosystem in an attempt to minimize sampling as a dependent variable in measuring the chemical and biological behavior of the ecosystem. If is intended that this sampling technology be used to directly measure the chemical behavior of a wide variety of organisms; including, plants, animals, and micro-organisms (e.g. algae, plankton). These probes facilitate the direct measurement of metabolism, decomposition, pollution, and stress or stimuli from the local environment. A variety of sampling tips and probes have been developed for discrete and continuous sampling. A variety of sampling probe geometries, sizes, and sampling capabilities are disclosed that enable both contact and non-contact sampling of the chemical environment. The liquid sampling has been optimized for chemical analysis with liquid chromatography mass spectrometry. Fatty acid and lipid profiling have been demonstrated on a number of species from a cultured aquatic using these techniques.



METHODS AND MATERIALS FOR DETECTING GENE AMPLIFICATION

Thu, 06 Apr 2017 08:00:00 EDT

This document relates to methods and materials involved in detecting gene amplification in a mammal. For example, methods and materials for detecting amplification at CPM and MDM2 loci to determine the presence or absence of a malignant lipomatous neoplasm in a mammal are provided.



APPLICATIONS OF SINGLE MOLECULE SEQUENCING

Thu, 06 Apr 2017 08:00:00 EDT

The invention provides methods for determining the presence of a disease by comparing a sequence from a single target molecule with a predetermined sequence that is associated with a specific disease.



GENE SIGNATURES FOR CANCER DIAGNOSIS AND PROGNOSIS

Thu, 06 Apr 2017 08:00:00 EDT

Biomarkers and methods using the biomarkers for molecular detection and classification of disease and, particularly, molecular markers for cancer diagnosis and prognosis and methods of use thereof are provided.



MULTI-COPY REFERENCE ASSAY

Thu, 06 Apr 2017 08:00:00 EDT

A method, comprising amplifying a nucleic acid sequence of interest in a sample comprising genomic DNA of a subject; amplifying a reference nucleic acid sequence in the sample; quantifying the amplified sequence of interest relative to the amplified reference sequence; and determining a copy number of the sequence of interest from the relative quantified amplified sequence of interest. The reference sequence may have at least 80% sequence identity to at least one of SEQ ID NO:1-38, such as SEQ ID NO:1-13. Also disclosed are kits and compositions, each comprising a first probe which specifically hybridizes to at least a portion of at least one reference sequence. Also disclosed is a system configured to perform the above method.



METHOD FOR DIAGNOSING COLORECTAL CANCER FROM A HUMAN FECES SAMPLE BY QUANTITIVE PCR, PRIMERS AND KIT

Thu, 06 Apr 2017 08:00:00 EDT

The present invention relates to the field of detection of colorectal cancer (CRC). Specifically it relates to methods for the early detection, risk screening and monitoring of CRC and/or adenomatous polyps in a human subject based on the quantification of one or more 16S rDNA bacterial sequences in feces. It further relates to the use of said bacterial sequences as biomarkers of colorectal cancer and/or adenomatous polyps; to a kit comprising a reagent and instructions for the quantification of said bacterial sequences; and to nucleic acids for the quantification of said 16S rDNA bacterial sequences.



METHODS FOR DIAGNOSING AND ASSESSING RISK OF DEVELOPING GLOMERULOSCLEROSIS

Thu, 06 Apr 2017 08:00:00 EDT

This document features method related to variants in the Inverted Formin 2 (INF2) gene that are associated with susceptibility to focal segmental glomerulosclerosis (FSGS). For example, methods of using such variants for risk assessment and for diagnosing and optimizing treatment of FSGS are provided.



MICROFLUIDIC DEVICES, SYSTEMS AND METHODS FOR SAMPLE PREPARATION AND ANALYSIS

Thu, 06 Apr 2017 08:00:00 EDT

The present disclosure provides microfluidic devices, systems and methods for sample preparation and/or analysis. A microfluidic device can include a first channel having a sequence of (n) chambers each having a first volume (v). The first channel can include one or more valves at opposing ends of the first channel that fluidically isolate the first channel. The microfluidic device can further include a second channel in fluid communication with the first channel. The second channel can include at least one second chamber having a total second volume that is at least equal to the total volume of the first channel (n*v). The second channel can include one or more valves at opposing ends of the second channel that fluidically isolate the second channel from the first channel.



DNA SEQUENCING BY NANOPORE USING MODIFIED NUCLEOTIDES

Thu, 06 Apr 2017 08:00:00 EDT

This invention provides a process for sequencing single-stranded DNA or RNA by employing a nanopore and modified nucleotides.



ANALYSIS OF A POLYMER FROM MULTI-DIMENSIONAL MEASUREMENTS

Thu, 06 Apr 2017 08:00:00 EDT

A target sequence of polymer units is estimated from plural series of measurements taken from sequences of polymer units that comprise the target sequence or a complementary sequence. Each measurement is dependent on a k-mer (k polymer units). Models treat the measurements as observations of k-mer states, comprising transition weightings in respect of transitions between successive k-mer states and emission weightings for different measurements being observed. An estimated alignment mapping between the plural series of measurements is derived based on an application of the models to each series. An estimate of the target sequence of polymer units is generated by applying the models, treating the types of k-mer state of each model and the measurements as dimensions of a plural dimensional k-mer state and plural dimensional observations. Constraint of paths through the plural dimensional k-mer states using the derived alignment mapping greatly reduces the required processing.



Isothermal Nucleic Acid Amplification Reactor With Integrated Solid State Membrane

Thu, 06 Apr 2017 08:00:00 EDT

Provided are devices adapted to isolate, amplify, and detect nucleic acids that may be present in a biological sample. The devices can, in some embodiments, isolate, amplify, and detect nucleic acid in a single chamber. In other embodiments, the devices are adapted to isolate and amplify nucleic acids in a reaction chamber, after which the nucleic acids may be communicated to a pervious medium—such as a lateral flow strip—where the user may label and detect the nucleic acids.



LABELED CIRCULAR DNA MOLECULES FOR ANALYSIS OF DNA TOPOLOGY, AND TOPOISOMERASES AND FOR DRUG SCREENING

Thu, 06 Apr 2017 08:00:00 EDT

The present invention provides labeled circular plasmid DNA molecules for studying DNA topology and topoisomerases. The molecules of the present invention also provide tools for high throughput drug screening for inhibitors of DNA gyrases and DNA topoisomerases for anticancer drug discovery and antibiotics discovery.



NOVEL PCR PRIMERS AND METHODS THEREOF FOR THE IDENTIFICATION OF BACILLUS COAGULANS

Thu, 06 Apr 2017 08:00:00 EDT

The present invention discloses novel oligonucleotide primer sequences BC1, BC2 and BC3 for the identification of Bacillus coagulans. The invention also discloses a PCR based method for the identification of Bacillus coagulants using the aforesaid primers, wherein positive amplification with primer sets BC1, BC2 and negative amplification with primer set BC3 confirms the presence of Bacillus coagulans.



METHOD FOR EVALUATING SUITABILITY OF DUODENAL FLUID SAMPLE AS SAMPLE FOR DETECTING PANCREATIC FLUID-DERIVED COMPONENTS

Thu, 06 Apr 2017 08:00:00 EDT

A method is provided for evaluating the suitability of a duodenal fluid sample collected from an animal as a sample for detecting pancreatic fluid-derived components. The method comprises: (a) mixing a duodenal fluid sample with a chymotrypsin-specific substrate and measuring an amount of degradation the chymotrypsin-specific substrate by of the duodenal fluid sample, (b) mixing the duodenal fluid sample with a pepsin-specific substrate and measuring an amount of degradation of the pepsin-specific substrate by the duodenal fluid sample, and (c) evaluating that the duodenal fluid sample is suitable as a sample for detecting pancreatic fluid-derived components if the amount of degradation of the chymotrypsin-specific substrate by the duodenal fluid sample is higher than a prescribed threshold value and the amount of degradation of the pepsin-specific substrate by the duodenal fluid sample is lower than a prescribed threshold value, as being suitable as a sample for detecting pancreatic fluid-derived components.



SUMOylation Assay and Related Reagents

Thu, 06 Apr 2017 08:00:00 EDT

Provided herein and methods and kits for identifying inhibitors of SUMOylation. The provided methods include contacting a candidate agent with (i) a SUMO-conjugating peptide comprising an N-terminal linker and a detectable tag, and (ii) a SUMO comprising a metal complex, under conditions that allow binding between the SUMO-conjugating peptide and the SUMO, and detecting the detectable tag thereby determining the level of binding between the SUMO-conjugating peptide and SUMO. A reduced level of binding as compared to the level of binding in the absence of the candidate agent indicating the agent inhibits SUMOylation.



REAGENT CARTRIDGE FOR DETECTION OF CELLS

Thu, 06 Apr 2017 08:00:00 EDT

An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. A delivery portion of the housing defines a delivery path between the reagent volume and the reaction chamber when the housing is coupled to the reaction chamber. The delivery path includes a protrusion such that the delivery path has a discontinuous inner surface. The actuator can be moved to convey a reagent from the reagent container into the reaction chamber via the delivery path.



Hybrid Suppressor tRNA for Vertebrate Cells

Thu, 06 Apr 2017 08:00:00 EDT

This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in vertebrate cells. The components include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNA's/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in vertebrate cells are also provided. The present invention provides vertebrate cells with translation components, e.g., pairs of orthogonal aminoacyl-tRNA synthetases (O-RSs) and orthogonal tRNA's (O-tRNA's) and individual components thereof, that are used in vertebrate protein biosynthetic machinery to incorporate an unnatural amino acid in a growing polypeptide chain, in a vertebrate cell.



Methods of Producing Rhamnolipids

Thu, 06 Apr 2017 08:00:00 EDT

The present invention relates to a method of preparing at least one rhamnolipid comprising: contacting a recombinant cell with a medium containing a carbon source; andculturing the cell under suitable conditions for preparation of the rhamnolipid from the carbon source by the cell,wherein the recombinant cell has been genetically modified such that, compared to the wild-type of the cell, the cell has an increased activity of at least one of the enzymes E1, E2 and E3, wherein the enzyme E1 is an α/β hydrolase, the enzyme E2 is a rhamnosyltransferase I and the enzyme E3 is a rhamnosyl-transferase II, andwherein the carbon source is a C4 molecule.



STRAND-INVASION BASED DNA AMPLIFICATION METHOD

Thu, 06 Apr 2017 08:00:00 EDT

Methods for amplification of a target nucleic acid sequence comprising strand invasion are provided in which strand invasion occurs both at upstream and downstream regions of the target nucleic acid sequence. Further provided are kits and compositions suitable for use in such methods. The methods may comprise amplifying a target nucleic acid sequence comprising a region of unknown sequence, or determining the sequence of a target nucleic acid comprising a region of unknown sequence.



Methods for Production of Xylosylxylitol Oligomers

Thu, 06 Apr 2017 08:00:00 EDT

The present disclosure relates generally to the production of xylosyl-xylitol oligomers, and more specifically to biological methods for producing xylosyl-xylitol oligomers in host cells.



CELL-FREE PREPARATION OF CARBAPENEMS

Thu, 06 Apr 2017 08:00:00 EDT

Provided herein are cell-free systems for generating carbapenems, e.g., a compound of the Formula (I): or salts thereof; wherein , R1, R2, R3, R4, R5 and R6 are defined herein. Also provided are pharmaceutical compositions comprising a compound generated by the inventive cell-free system, and use of these compounds and compositions for the treatment of bacterial infections.



SELECTIVE ADVANTAGE IN FERMENTATION

Thu, 06 Apr 2017 08:00:00 EDT

Disclosed are transformed cells and related nucleotide and protein sequences, and fermentation compositions and methods, all of which are related to providing selective advantage in fermentation. For example, a selective advantage results from transformation of a cell with a nucleic acid that allows a transformed cell to metabolize one or more nitrogen-, phosphorous-, and/or sulfur-containing compounds that a native cell of the same species as the transformed cell cannot metabolize, and from fermentation of the transformed cell using one or more feedstocks, such as fractioned grain, which are depleted in or free of conventional nitrogen-, phosphorous-, and/or sulfur-containing compounds that a native cell of the same species as the transformed cell can metabolize. Also disclosed are methods for improved oxygen transfer in an aerobic or microaerobic fermentation.



Means and Methods for Itaconic Acid Production

Thu, 06 Apr 2017 08:00:00 EDT

The present invention relates to a method of producing itaconic acid. Further the present invention relates to nucleic acids encoding an aconitate-delta-isomerase (ADI) and trans-aconitate decarboxylase (TAD) and uses of such nucleic acids. Provided is additionally a recombinant host cell engineered to overexpress nucleic acids of the present invention. Furthermore an expression cassette and a vector are provided which include the respective nucleic acid.



SEMI-SYNTHETIC TEREPHTHALIC ACID VIA MICROORGANISMS THAT PRODUCE MUCONIC ACID

Thu, 06 Apr 2017 08:00:00 EDT

The invention provides a non-naturally occurring microbial organism having a muconate pathway having at least one exogenous nucleic acid encoding a muconate pathway enzyme expressed in a sufficient amount to produce muconate. The muconate pathway including an enzyme selected from the group consisting of a beta-ketothiolase, a beta-ketoadipyl-CoA hydrolase, a beta-ketoadipyl-CoA transferase, a beta-ketoadipyl-CoA ligase, a 2-fumarylacetate reductase, a 2-fumarylacetate dehydrogenase, a trans-3-hydroxy-4-hexendioate dehydratase, a 2-fumarylacetate aminotransferase, a 2-fumarylacetate aminating oxidoreductase, a trans-3-amino-4-hexenoate deaminase, a beta-ketoadipate enol-lactone hydrolase, a muconolactone isomerase, a muconate cycloisomerase, a beta-ketoadipyl-CoA dehydrogenase, a 3-hydroxyadipyl-CoA dehydratase, a 2,3-dehydroadipyl-CoA transferase, a 2,3-dehydroadipyl-CoA hydrolase, a 2,3-dehydroadipyl-CoA ligase, a muconate reductase, a 2-maleylacetate reductase, a 2-maleylacetate dehydrogenase, a cis-3-hydroxy-4-hexendioate dehydratase, a 2-maleylacetate aminoatransferase, a 2-maleylacetate aminating oxidoreductase, a cis-3-amino-4-hexendioate deaminase, and a muconate cis/trans isomerase. Other muconate pathway enzymes also are provided. Additionally provided are methods of producing muconate.



XYLANASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME

Thu, 06 Apr 2017 08:00:00 EDT

The present invention relates to xylanase variants, comprising an alteration at least at one position corresponding to position 87 of the polypeptide of SEQ ID NO: 3, wherein the variant has xylanase activity and has increased xylanase inhibitor tolerance compared to the xylanase of SEQ ID NO: 3; and i) wherein the variant has at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide of SEQ ID NO: 3; or ii) wherein the number of alterations is 1-20, e.g., 1-10 such as 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 alterations. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.



TRANSGENIC TOBACCO PLANTS FOR ENHANCED BIOETHANOL PRODUCTION

Thu, 06 Apr 2017 08:00:00 EDT

Genetically modified tobacco plants are provided having altered hexose accumulation. Methods are provided for producing ethanol from fermentation of tobacco biomass derived from the tobacco plants having altered hexose accumulation. The altered hexose accumulation can be an increase in total hexose content or an increase in hexose content in the phloem or the roots/shoots as compared to non-genetically modified tobacco plants. Expression vectors are provided for tobacco plant transformation having a gene encoding a sucrose invertase inhibitor operably linked to a promoter, such that expression of the inhibitor in the plant can increase and/or alter hexose accumulation in the plant. The genetically modified tobacco plants having altered hexose accumulation can further contain a transgenic construct to confer resistance to a glyphosate herbicide or a phosphinothricin (PPT) herbicide.



Microorganisms for Biosynthesis of Limonene on Gaseous Substrates

Thu, 06 Apr 2017 08:00:00 EDT

Engineered microorganisms are provided that convert gaseous substrates, such as producer gas, into limonene. In some embodiments, limonene is pumped out of the cell via an efflux pump. In some embodiments, limonene, produced as described herein, is converted through catalytic dimerization into jet fuel. Producer gas used in the processes described herein for production of limonene may be derived from sources that include gasification of waste feedstock and/or biomass residue, waste gas from industrial processes, or natural gas, biogas, or landfill gas.



ENHANCED EXPRESSION AND STABILITY REGIONS

Thu, 06 Apr 2017 08:00:00 EDT

Expression-enhancing nucleotide sequences for expression in eukaryotic systems are provided that allow for enhanced and stable expression of recombinant proteins in eukaryotic cells. Enhanced expression and stability regions (EESYRs) are provided for expression of a gene of interest in a eukaryotic cell. Chromosomal loci, sequences, and vectors are provided for enhanced and stable expression of genes in eukaryotic cells.



EUKARYOTIC EXPRESSION VECTORS RESISTANT TO TRANSGENE SILENCING

Thu, 06 Apr 2017 08:00:00 EDT

The present invention relates to recombinant DNA molecules such as plasmids, non-viral vectors, viral vectors and hybrids thereof, and more particularly to vector modifications that improve expression of said DNA molecules in cell lines and organisms.



FABRICATION OF HIERARCHICAL SILICA NANOMEMBRANES AND USES THEREOF FOR SOLID PHASE EXTRACTION OF NUCLEIC ACIDS

Thu, 06 Apr 2017 08:00:00 EDT

The present invention provides a novel method to fabricate silica nanostructures on thin polymer films based on silica deposition and self-wrinkling induced by thermal shrinkage. These micro- and nano-scale structures have vastly enlarged the specific area of silica, thus the silica nanomembranes can be used for solid phase extraction of nucleic acids. The inventive silica nanomembranes are suitable for nucleic acid purification and isolation and demonstrated better performance than commercial particles in terms of DNA recovery yield and integrity. In addition, the silica nanomembranes have extremely high nucleic acid capacity due to its significantly enlarged specific surface area of silica. Methods of use and devices comprising the silica nanomembranes are also provided.



METHODS AND DEVICES FOR SIMULTANEOUS OPTICAL IRRADIATION AND OSCILLATING MAGNETIC FIELD RADIATION OF A TARGET

Thu, 06 Apr 2017 08:00:00 EDT

The present disclosure is generally directed to methods and devices for the precise and simultaneous optical irradiation and oscillating magnetic field radiation of a target, such as mammalian cells and/or nanostructures.



MESOPOROUS CATALYSTS OF MAGNETIC NANOPARTICLES AND FREE-RADICALPRODUCING ENZYMES, AND METHODS OF USE

Thu, 06 Apr 2017 08:00:00 EDT

A composition comprising mesoporous aggregates of magnetic nanoparticles and free-radical producing enzyme (i.e., enzyme-bound mesoporous aggregates), wherein the mesoporous aggregates of magnetic nanoparticles have mesopores in which the free-radical-producing enzyme is embedded. Methods for synthesizing the enzyme-bound mesoporous aggregates are also described. Processes that use said enzyme-bound mesoporous aggregates for depolymerizing lignin, removing aromatic contaminants from water, and polymerizing monomers polymerizable by a free-radical reaction are also described.



PROCESSES FOR PREPARING SILICA-CARBON ALLOTROPE COMPOSITE MATERIALS AND USING SAME

Thu, 06 Apr 2017 08:00:00 EDT

The present document describes a carbon allotrope-silica composite material comprising a silica microcapsule comprising a silica shell having a thickness of from about 50 nm to about 500 μm, and a plurality of pores, said shell forming a capsule having a diameter from about 0.2 μm to about 1500 and having a density of about 0.001 g/cm3 to about 1.0 g/cm3, wherein said shell comprises from about 0% to about 70% Q3 configuration, and from about 30% to about 100% Q4 configuration, or wherein said shell comprises from about 0% to about 60% T2 configuration and from about 40% to about 100% T3 configuration, or wherein said shell comprises a combination of T and Q configurations thereof, and wherein an exterior surface of said capsule is covered by a functional group; a carbon allotrope attached to said silica microcapsule. Also described is a carbon allotrope-silica composite material comprising a carbon allotrope attached to a silica moiety comprising a silica nanoparticle having a diameter from about 5 nm to about 1000 nm, wherein an exterior surface of said silica nanoparticle is covered by a functional group.



THERMOSTABLE ALGINATE DEGRADING ENZYMES AND THEIR METHODS OF USE

Thu, 06 Apr 2017 08:00:00 EDT

The present invention relates to the identification, production and use of thermostable alginate lyase enzymes that can be used to partially degrade alginate to yield oligosaccharides or to give complete degradation of alginate to yield (unsaturated) mono-uronates.



SERINE PROTEASES OF BACILLUS SPECIES

Thu, 06 Apr 2017 08:00:00 EDT

The present disclosure relates to serine proteases cloned from Bacillus spp., and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.



POLYPEPTIDE HAVING PROTEASE ACTIVITY AND METHODS FOR INCREASING ITS ACTIVITY THEREOF

Thu, 06 Apr 2017 08:00:00 EDT

The present invention relates to a polypeptide having protease activity comprising a zinc finger protease domain, a helix-turn-helix domain and a GAF domain. The core protein sequence of the protease is shown as SEQ ID NO: 1. The invention also relates to optimized reaction conditions for the protease and methods of increasing the protease activity.



METHOD FOR REDUCING VISCOSITY IN SACCHARIFICATION PROCESS

Thu, 06 Apr 2017 08:00:00 EDT

The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having glycosyl hydrolase family 61/endoglucanase activity, methods for hydrolyzing biomass material, and methods for reducing viscosity of biomass mixture using a composition comprising a polypeptide having glycosyl hydrolase family 61/endoglucanase activity.



Alpha-Amylase Variants and Polynucleotides Encoding Same

Thu, 06 Apr 2017 08:00:00 EDT

The present invention relates to alpha-amylase variants with improved stability in the presence of glucose and/or relatively higher activity on long chain versus short chain substrates. The present encoding invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.



POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME

Thu, 06 Apr 2017 08:00:00 EDT

The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.



CYCLODEXTRIN GLUCANOTRANSFERASE

Thu, 06 Apr 2017 08:00:00 EDT

The present invention relates to a novel cyclodextrin glucanotransferase (CGTase) enzyme which is obtainable from Clostridium saccharoperbutylacetonicum N1-4, N1-4(HMT) or N1-504. The invention further relates to nucleic acids encoding the enzyme, vectors and host cells, and uses of the CGTase.



Modified Adenovirus Hexon Protein and Uses Thereof

Thu, 06 Apr 2017 08:00:00 EDT

The present invention provides a method of altering the specificity of an adenovirus vector. The method involves providing an adenovirus having a capsid with a modified adenovirus hexon protein. The modified adenovirus has a capsid comprising a hexon protein with a deletion in hypervariable region 1 and/or hypervariable region 4 of the hexon and an insert of an exogenous molecule therein.



Bionanomaterials and Their Synthesis

Thu, 06 Apr 2017 08:00:00 EDT

The use of biomaterials, such as viruses and virus-like particles, to form nanostructures is generally disclosed. For instance, rod-like viruses can be used to form composite nanofibers that are fixed together in a head-to-tail assembly by a polymer. Also, 2-dimensional nanostructures formed from crosslinked viruses assembled in a single, film-like layer are generally disclosed. Porous gels having controllable pore size through the use of virus particles are also disclosed.



METHOD FOR CELL CULTURE

Thu, 06 Apr 2017 08:00:00 EDT

A method for stem or progenitor cell culture. More precisely, the invention relates to a method for cell culture using one or more IαI (inter-alpha trypsin inhibitor or Inter-alpha inhibitor) protein(s) or part(s) thereof as a component in a cell culture media or a coating on a cell culture surface material. Furthermore the invention relates to a cell culture media and a cell culture coating/matrix provided with one or more IαI proteins(s) or part(s) thereof.



PRIMITIVE AND PROXIMAL HEPATIC STEM CELLS

Thu, 06 Apr 2017 08:00:00 EDT

Hepatic progenitors comprise two populations of human hepatic stem cells, primitive and proximal hepatic stem cells, and two populations of committed progenitors, one for biliary cells and one for hepatocytes. Human primitive hepatic stem cells are a very small fraction of the liver cell population and give rise to proximal hepatic stem cells constituting a much larger fraction of the liver. Human proximal hepatic stem cells give rise to biliary and hepatocyte committed progenitors. Primitive and proximal stem cells are the primary stem cells for the human liver. Human primitive hepatic stem cells may be isolated by immunoselection from human livers or culturing human liver cells under conditions which select for a human primitive hepatic stem cell. Proximal hepatic stem cells may be isolated by immunoselection, or by culturing human liver cells under conditions which include a developmental factor. Proximal hepatic stem cells may also be isolated by culturing colonies comprising a primitive hepatic stem cell under conditions which include a developmental factor. Resulting compositions may be used for treating liver disorders and for producing bioartificial organs.



Mature Dendritic Cell Compositions and Methods for Culturing Same

Thu, 06 Apr 2017 08:00:00 EDT

This invention provides methods to prepare and use immunostimulatory cells for enhancing an immune response. The invention provides a method for preparing mature dendritic cells (DCs), comprising the sequential steps of: (a) signaling isolated immature dendritic cells (iDCs) with a first signal comprising an interferon gamma receptor (IFN-γR) agonist and/or a tumor necrosis factor alpha receptor (TNF-αR) agonist to produce signaled dendritic cells; and (b) signaling said signaled dendritic cells with a second transient signal comprising an effective amount of a CD40 agonist to produce CCR7+ mature dendritic cells. Also provided by this invention are enriched populations of dendritic cells prepared by the methods of the invention. Such dendritic cells have enhanced immunostimulatory properties and increased IL-12 secretion and/or decreased IL-10 secretion. CD40 signaling can be initiated by one or more of polypeptide translated from an exogenous polynucleotide encoding CD40L (e.g., mRNA or DNA), an agonistic antibody to CD40 receptor or by CD40 ligand polypeptide. The enriched populations can be further modified by the administration of an immunogen to the DC. The DC will take up and process the immunogen on its cell surface.



Mature Dendritic Cell Compositions and Methods for Culturing Same

Thu, 06 Apr 2017 08:00:00 EDT

This invention provides methods to prepare and use immunostimulatory cells for enhancing an immune response. The invention provides a method for preparing mature dendritic cells (DCs), comprising the sequential steps of: (a) signaling isolated immature dendritic cells (iDCs) with a first signal comprising an interferon gamma receptor (IFN-γR) agonist and/or a tumor necrosis factor alpha receptor (TNF-αR) agonist to produce signaled dendritic cells; and (b) signaling said signaled dendritic cells with a second transient signal comprising an effective amount of a CD40 agonist to produce CCR7+ mature dendritic cells. Also provided by this invention are enriched populations of dendritic cells prepared by the methods of the invention. Such dendritic cells have enhanced immunostimulatory properties and increased IL-12 secretion and/or decreased IL-10 secretion. CD40 signaling can be initiated by one or more of polypeptide translated from an exogenous polynucleotide encoding CD40L (e.g., mRNA or DNA), an agonistic antibody to CD40 receptor or by CD40 ligand polypeptide. The enriched populations can be further modified by the administration of an immunogen to the DC. The DC will take up and process the immunogen on its cell surface.



Reducing Immune Tolerance Induced by PD-L1

Thu, 06 Apr 2017 08:00:00 EDT

The present disclosure relates to compositions and methods for reducing immune tolerance associated with CAR T cell therapy. Embodiments of the present disclosure include isolated nucleic acid sequence comprising a nucleic acid sequence that encodes modified programmed cell death protein 1 (PD-1) and a nucleic acid sequence that encodes chimeric antigen receptor (CAR).



Zwitterionic-Bias Material for Blood Cell Selection and Application Thereof

Thu, 06 Apr 2017 08:00:00 EDT

The invention provides a zwitterionic-bias material for blood cell selection and a method for removing leukocytes from a blood sample. The zwitterionic-bias material for blood cell selection is a copolymer formed by zwitterionic structural units and charged structural units wherein the zwitterionic structural unit comprises at least one positively charged moiety and one negatively charged moiety, a distance between the positively charged moiety and the negatively charged moiety is a length of 1˜5 carbon-carbon bonds, and the zwitterionic structural units and charged structural units are randomly arranged to have zwitterionic-bias.



MYCOBACTERIA PRE-ANALYTIC REAGENT

Thu, 06 Apr 2017 08:00:00 EDT

A pre-analytic reagent is provided for liquefaction of a sputum sample and complete inactivation of one or more Mycobacteria, e.g., Mycobacterium tuberculosis (MTB) suspected of being contained in the sputum sample at room temperature. The pre-analytic reagent may include one or more of each of: a chaotropic agent, a chelator, a reducing agent, a detergent, and acetic acid. The pre-analytic reagent can be configured to be effective to liquefy the sputum sample and completely inactivate the Mycobacteria suspected to be contained therein.



HIGH SALINITY TOLERANT MICROALGAE STRAINS, PRODUCTS DERIVED THEREFROM, AND METHODS OF PRODUCING THE SAME

Thu, 06 Apr 2017 08:00:00 EDT

The present invention relates generally to microalgae strains that are tolerant to high salinity, the products derived from the high salinity tolerant microalgae strains, and methods of producing high salinity tolerant microalgae strains and their products.



INLET AND OUTLET GEOMETRIES FOR A VERTICAL THREE-STREAM MICROFLUIDIC DEVICE

Thu, 06 Apr 2017 08:00:00 EDT

An example includes an apparatus for separating cells from a fluid sample, including a plenum, defining: a centrally located top inlet, and respective side outlets disposed below the inlet and to the side of the inlet; and a bottom outlet disposed below the top inlet, to the side of the two or more outlets, wherein the plenum is configured to receive a fluid and cells suspension through the inlet, and direct it to the bottom outlet, against respective side-walls extending between the bottom outlet and the two or more side outlets, and wherein the distance between each of the two or more side outlets and the bottom outlet is selected to encourage cells to exit through the bottom outlet under a force. Lateral/vertical distance between the inlet and side outlets can provide lateral fluid travel, thereby allowing time for gravity to bias cells toward an outlet.



Test Apparatus

Thu, 06 Apr 2017 08:00:00 EDT

Provided is a test apparatus in which a test for bacterial identification or antimicrobial susceptibility can be promptly determined. A division state of bacteria is monitored by performing microscopic observation of shapes and the number of the bacteria in each of wells in a culture plate for bacterial identification culture or an antimicrobial susceptibility test, and it is determined whether or not the bacteria grow in a stage shifted from an induction phase to a logarithmic phase, with reference to an image obtained through microscopic observation. In addition, determination performed based on turbidity in the related art may be combined with determination performed based on microscopic observation in which change and the like in the shapes of the bacteria are monitored. Accordingly, it is possible to realize a highly accurate test result.



COMPACT SUBNANOSECOND HIGH VOLTAGE PULSE GENERATION SYSTEM FOR CELL ELECTRO-MANIPULATION

Thu, 06 Apr 2017 08:00:00 EDT

Disclosed are methods and systems for subnanosecond rise time high voltage (HV) electric pulse delivery to biological loads. The system includes an imaging device and monitoring apparatus used for bio-photonic studies of pulse induced intracellular effects. The system further features a custom fabricated microscope slide having micro-machined electrodes. A printed circuit board to interface the pulse generator to the micro-machined glass slide having the cell solution is disclosed. An low-parasitic electronic setup to interface with avalanche transistor-switched pulse generation system is also disclosed. The pc-board and the slide are configured to match the output impedance of the pulse generator which minimizes reflection back into the pulse generator, and minimizes distortion of the pulse shape and pulse parameters. The pc-board further includes a high bandwidth voltage divider for real-time monitoring of pulses delivered to the cell solutions.



FILTRATION DEVICE AND SYSTEM

Thu, 06 Apr 2017 08:00:00 EDT

The invention relates to a filtration device (100), characterized in that it includes: a first block (101) having a cavity forming a first chamber (110) comprising a bottom wall (111) having a set of microstructures including micro-walls and micro-contacts, the set of microstructures defining micro-chambers and micro-channels on the bottom wall (111) of the culture chamber; a second block (102) having a cavity forming a second chamber (120); and a filtration membrane (130), the first block (101), the membrane (130), and the second block (102) being arranged such that the membrane (130) is located between the first chamber (110) and the second chamber (120), adjacent to each of the first and second chambers (110, 120); as well as a first opening and a second opening for enabling a first fluid to pass into the second chamber (120) which is separated from the first chamber (110) by the membrane (130).



Conical Impeller and Applications Thereof

Thu, 06 Apr 2017 08:00:00 EDT

A conical impeller with a hub that has a conical surface extending into the interior of the impeller. The hub has spiral, slanting arms which are attached or integrally formed with a plurality of curved blades. The blades can be connected at the bottom by a ring. The intersection of the conical surface of the hub with the blade forms an upward path for fluids and their entrained particles or gases which have been brought into the interior of the impeller to effectively completely be ejected. The discharge edges of the blades and/or the conical surface of the hub may have openings for discharging gas into the fluid. The impeller imparts low shear to the fluid and its components, and the shear is independent or only depends slightly on the size of the impeller. The impeller minimizes or eliminates particle agglomeration and fouling of the impeller. The efficiency of and flow pattern produced by the impeller means that containers don't require the use of baffles. The high efficiency and low shear of the impeller is useful for applications such as mixing of biological fluids, decontamination of produced water, chemical mechanical polishing, and flotation cells. The impeller may be raised or lowered in the mixing vessel, and may incorporate embedded sensors.



ADVANCED TISSUE ENGINEERING SYSTEM

Thu, 06 Apr 2017 08:00:00 EDT

The invention is an automated advanced tissue engineering system that comprises a housing in which one or more tissue engineering modules are accomodated together with a central microprocessor that controls functioning of the tissue engineering modules. In one embodiment, the tissue engineering module comprises a housing supporting one or more bioreactor chamber assemblies and a fluid reservoir operationally engageable with the housing. The bioreactor chamber assemblies may be selected depending on the end product option desired and may include, for example, a cell therapy bioreactor chamber, a single implant bioreactor chamber and a multiple (mosaic) implant bioreactor chamber.



Polypeptides Having Phospholipase C Activity and Polynucleotides Encoding Same

Thu, 06 Apr 2017 08:00:00 EDT

The present invention provides polypeptides having phospholipase C activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides. Furthermore, the present invention provides a method of reducing the phospholipid content in an oil composition using the polypeptide having phospholipase C activity as well as other relevant uses of the polypeptide.



Polypeptides Having Phospholipase C Activity and Polynucleotides Encoding Same

Thu, 06 Apr 2017 08:00:00 EDT

The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.



ANTIBODIES SPECIFICALLY BINDING TO HER3

Thu, 06 Apr 2017 08:00:00 EDT

The present invention relates to isolated antibodies, or an antigen portions thereof, which bind to human HER3. The novel antibodies are of great utility since they allow for the sensitive and specific detection of human HER3. Detection of human HER3 is, e.g., possible in a tissue sample, even when such tissue sample is a formalin-fixed paraffin embedded tissue (FFPET) sample.



METHODS AND COMPOSITIONS RELATED TO AMYLOID-BETA-42 OLIGOMERS

Thu, 06 Apr 2017 08:00:00 EDT

Embodiments are directed to compositions comprising a conformation-dependent antibody that specifically binds oligomeric Aβ-42, and the methods of using the same.



Modified FGF-21 Polypeptides and Their Uses

Thu, 06 Apr 2017 08:00:00 EDT

Modified FGF-21 polypeptides and uses thereof are provided.



PEPTIDE TAGS FOR LABELING PROTEINS BY FUSION, AND ANTIBODIES FOR THE DETECTION THEREOF

Thu, 06 Apr 2017 08:00:00 EDT

Peptide tags (tags) are provided for their fusion to proteins at the N-terminal or C-terminal ends, which are defined by a series of structural and functional properties that define their safety with regard to the modification of the structure or biological function of the protein or peptide to which they are fused, and wherein the tag-protein is selected from a group consisting of a fragment of Phl P 2 Pheleum pratense , a fragment of Hev b 6.02 Hevea brasiliensis, and a fragment of Amb t 5 Ambrosia trifida, or a combination thereof. Methods are also provided for the production and detection of these recombinant fusion proteins as well as specific antibodies that bind to these tags.



SAMPLE COLLECTION AND PROCESSING DEVICE

Thu, 06 Apr 2017 08:00:00 EDT

device for separating and concentrating target particles or molecules from a fibrinogen containing sample of liquid comprises a container (1) for collecting the sample and a closure (2). The container (1) comprises a first end and a second end and at least one interior wall defining a reservoir portion (5) for receiving the sample. The reservoir portion (5) comprises at least one anchor element (4) to locally catch a polymerized fibrin pellet formed upon the addition of the sample into the container. The separation and concentration process is operated by trapping the target particles or molecules into the so-formed polymerized fibrin pellet that are captured on the anchor element (4).



HANDHELD FLUID HANDLING SYSTEMS AND METHODS

Thu, 06 Apr 2017 08:00:00 EDT

A handheld system includes a reference pressure source configured to generate a reference pressure. The handheld system also includes a primary pressure source coupled to the reference pressure source. The primary pressure source is configured to generate a primary pressure in a primary pressure range. The primary pressure is less than the reference pressure, and the primary pressure is induced by the reference pressure source. The handheld system also includes a secondary pressure source coupled to the primary pressure source. The secondary pressure source is configured to generate a secondary pressure in a secondary pressure range. The secondary pressure is less than the primary pressure, and the secondary pressure is induced by the primary pressure source.



FILTRATION DEVICE FOR RAPID SEPARATION OF BIOLOGICAL PARTICLES FROM COMPLEX MATRICES

Thu, 06 Apr 2017 08:00:00 EDT

Methods and systems for filtering of biological particles are disclosed. Filtering membranes separate adjacent chambers. Through osmotic or electrokinetic processes, flow of particles is carried out through the filtering membranes. Cells, viruses and cell waste can be filtered depending on the size of the pores of the membrane. A polymer brush can be applied to a surface of the membrane to enhance filtering and prevent fouling.



ULTRA-RAPID TISSUE CRYOPRESERVATION METHOD AND APPARATUS

Thu, 06 Apr 2017 08:00:00 EDT

A method and apparatus for the processing of tissue and cellular material during cryopreservation and/or processing for microscopy. The method and apparatus maximizes heat transfer coefficients by using liquid-free cryopreservation protocols and maximizing glass transition characteristics through increasing pressure during cryopreservation. Cooling rates combined with megapascal pressures reduced the required concentration of cryoprotective agents (CPAs) needed for ice-free cell and tissue cryopreservation.



PHOTON MODULATION MANAGEMENT SYSTEM

Thu, 06 Apr 2017 08:00:00 EDT

Embodiments described herein provide systems for optimizing organism growth, destruction or repair, by controlling the duty cycle, wavelength band and frequency of photon bursts to an organism, through the high frequency modulation of photons in an individual color spectrum to the organism, where the photon modulation is based upon the specific needs of the organism. Devices for the optimization of organism growth, destruction or repair through the high frequency modulation of photons of individual color spectrum to the organism are also provided. Further provided are methods for the optimization of organism growth, destruction or repair through the use of high frequency modulation of photons of individual color spectrums.