Thu, 25 Aug 2016 08:00:00 EDTAn object of the present invention is to provide a means for efficiently obtaining mammalian embryos having high conception rates. A first aspect of the present invention is a method for selecting a mammalian embryo prepared by in vitro culture from a fertilized egg, comprising a step of selecting an embryo using two or more of the following indicators: the time from fertilization to the completion of first cleavage; the morphology at a stage after first cleavage and before second cleavage; the morphology at a stage after third cleavage and before fourth cleavage; and the amount of oxygen consumed at the early blastocyst stage, the blastocyst stage, or the expanded blastocyst stage.
Thu, 25 Aug 2016 08:00:00 EDTA handheld diagnostic system may include a disposable sample holder for receiving and containing a biological sample and an analysis module having a chip-scale microscope. The sample holder may include a plurality of uniformly spaced tick marks. The analysis module may include a sensor for detecting the tick marks as the sample holder is inserted into the analysis module. The chip-scale microscope may include an image sensor for capturing images of the sample. Each time the sensor detects a tick mark, control circuitry may issue a control signal to the image sensor to capture an image of the biological sample. This type of automated image capture mechanism ensures that images are captured at a uniform spatial distribution even when the sample holder is inserted into the analysis module at variable speed. The analysis module may transmit sample imaging data to a portable electronic device.
Thu, 25 Aug 2016 08:00:00 EDTThe present invention provides an efficient apparatus for extracting biologically active substances through a magnetic particle method, comprising a magnetic needle, a magnetic needle moving mechanism, a sleeve and a sleeve moving mechanism and a vibration mechanism, a bracket, a positioning mechanism; the vibration mechanism is capable of generating vibrations to enable the sleeve moving mechanism to make upward and downward vibrations; wherein the magnetic needle can vertically insert into and move out of the sleeve; the sleeve can vertically insert into and move out of a sample treatment area; and the vibration mechanism comprises a lever bar, a movable fulcrum and a rotary mechanism. The present invention further provides an instrument comprising the apparatus for extracting biologically active substances. The present invention further provides a method for extracting and purifying biologically active substances using the apparatus or the instrument for extracting biologically active substances. The vibration frequency and amplitude of the apparatus and the instrument can be optionally adjusted, and the range of adjustments for the amplitude is greatly extended, so the apparatus and the instrument exhibit such advantages as making only small noise and have a long service life.
Thu, 25 Aug 2016 08:00:00 EDTAn apparatus including at least one of a stainer module and a coverslipper module; an imaging module; a storage module; an automated transport module for transporting at least one slide between at least one of the stainer module and the coverslipper module, the imaging module and the storage module; and a controller. A method including processing at least one slide; determining whether an imaging module is available for imaging of a biological specimen on the at least one slide; transporting the at least one slide to the imaging module using an automated transport module; and transporting the at least one slide to a storage module using the automated transport module when it is determined that the imaging module is not available. A system including a processing module for processing at least one slide including a biological specimen thereon. A machine readable medium.
Thu, 25 Aug 2016 08:00:00 EDTA method for constructing an metal ion binding motif by identifying an metal ion binding peptide that binds an metal ion with specificity, ascertaining at least a portion of a nucleic acid sequence encoding the metal ion binding peptide, tailoring the nucleic acid sequence encoding the metal ion binding peptide into an metal ion binding site, identifying a host protein and a relevant portion of the nucleic acid sequence of the host protein, operatively linking the tailored nucleic acid sequence encoding the metal ion binding peptide and the host protein nucleic acid sequence into an metal ion binding motif sequence, and expressing metal ion binding motif sequence, in which the nucleic acid sequence encoding the metal ion binding peptide is tailored so as to achieve the metal ion binding motif with a desired specificity for the metal ion. Also, the proteins encoded by the metal ion binding motif sequence as constructed by the method.
Thu, 25 Aug 2016 08:00:00 EDTThe present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in appendicitis patients and in patients at risk for appendicitis. In particular, the invention relates to using assays that detect one or more biomarkers as diagnostic and prognostic biomarker assays in such patients.
Thu, 25 Aug 2016 08:00:00 EDTMethods and kits for diagnosing the presence of and prognosing the appearance of tissue remodelling-associated conditions, involving the presence of enzyme complexes in a biological sample, are disclosed. In particular, the method pertains to diagnosing the presence of or prognosing appearance of metastatic cancer by the identification of high molecular weight enzyme complexes comprising MMPs.
Thu, 25 Aug 2016 08:00:00 EDTCompositions and methods which indicate an increased risk for pancreatic carcinoma in a test subject are disclosed.
Thu, 25 Aug 2016 08:00:00 EDTThe invention features methods of diagnosis by assessing B7-H1 expression in a tissue from a subject that has, or is suspected of having, cancer, methods of treatment with agents that interfere with B7-H1-receptor interaction, methods of selecting candidate subjects likely to benefit from cancer immunotherapy, and methods of inhibiting expression of B7-H1.
Thu, 25 Aug 2016 08:00:00 EDTIt is intended to provide a method for detecting a pancreatic tumor (pancreatic cancer or benign pancreatic tumor) with low invasiveness to a test subject and with high detection sensitivity and accuracy. The present invention provides a method for detecting a pancreatic tumor by measuring the amounts of APOA2 protein variants in a sample of a test subject using anti-APOA2 antibodies, anti-APOA2 antibodies for use in the method, and a kit for the detection of a pancreatic tumor, comprising the antibodies.
Thu, 25 Aug 2016 08:00:00 EDTThe present invention relates to a method for diagnosis of cancer and for monitoring the progression of cancer and/or the therapeutic efficacy of an anti-cancer treatment in a sample of a subject by detecting oncogenic proteins in microvesicles, and to the use of an agent blocking exchange of microvesicles for treating cancer.
Thu, 25 Aug 2016 08:00:00 EDTThe present disclosure relates to compositions and methods for diagnosis, research, and screening for chemicals in biological fluids (e.g., related to methanol poisoning, ethanol levels, and ethylene glycol poisoning). In particular, the present disclosure relates to point of care systems and methods for detecting formic acid or formate, ethanol, ethylene glycol, and other clinically relevant chemicals in biological fluids.
Thu, 25 Aug 2016 08:00:00 EDTSystems, methods, and devices for detecting infections in a clinical sample are provided. Small-volume clinical samples obtained at a point-of-service (POS) location and may be tested at the POS location for multiple markers for multiple diseases, including upper and lower respiratory diseases. Samples may be tested for cytokines, or for inflammation indicators. Dilution of samples, or levels of detection, may be determined by the condition or past history of a subject. Test results may be obtained within a short amount of time after sample placement in a testing device, or within a short amount of time after being obtained from the subject. A prescription for treatment of a detected disorder may be provided, and may be filled, at the POS location. A bill may be automatically generated for the testing, or for the prescription, may be automatically sent to an insurance provider, and payment may be automatically obtained.
Thu, 25 Aug 2016 08:00:00 EDTThe present invention relates to compositions and method for diagnosing cancer by detecting IL13Rα2 or a tumor associated exosome. In some instances, the invention relates to using IL13-conjugated quantum dots to detect an IL13Rα2 or a tumor associated exosome.
Thu, 25 Aug 2016 08:00:00 EDTMethods of identifying a subject having an autoimmune disease, such as Type 1 diabetes, as likely to respond to treatment with a tumor necrosis factor-alpha (TNF-α) receptor II activator, involving measuring CD8 protein density on the surface of autoreactive CD8+ T cells and identifying the subject as likely to respond to the treatment if the CD8 protein density is reduced relative to a reference CD8+ T cell. For Type 1 diabetes, the method may involve measuring C-peptide levels in an in vitro biological sample from the subject, identifying the subject as likely to respond to the treatment if the C-peptide levels are detectable, and identifying the subject as unlikely to respond to the treatment if the C-peptide levels are substantially undetectable. The invention also features pharmaceutical compositions of one or more TNFR2 activators for therapeutic use.
Thu, 25 Aug 2016 08:00:00 EDTA lateral flow assay detects and differentiates between viral and bacterial infections. A combined point of care diagnostic device tests markers for viral infection and markers for bacterial infection, to effectively assist in the rapid differentiation of viral and bacterial infections. In one preferred embodiment, the bacterial marker is CRP. In another preferred embodiment, the viral marker is MxA. In some embodiments, it is unnecessary to lyse the cells in the sample prior to applying it to the device.
Thu, 25 Aug 2016 08:00:00 EDTHomogeneous immunoassays that allow for compensation of background signals inherent in samples and reagents. The use of homogeneous immunoassays for the detection of the presence or amount of symmetrical Dimethyl Arginine (SDMA) in biological samples. Reagents and kits for conducting the assays.
Thu, 25 Aug 2016 08:00:00 EDTAntibodies, immunoassay methods and kits for the detection and determination of 3,4,-dichloro-N-[(1-(dimethylamino)cyclohexyl)methyl]benzamide and 3,4,-dichloro-N-[(1-(methylamino)cyclohexyl)methyl]benzamide, as well as the precursory immunogens, are described.
Thu, 25 Aug 2016 08:00:00 EDTThe present invention provides a detection instrument capable of easily detecting an intended detection object without any skilled technique. The detection instrument (1) of the present invention includes a detection portion (12), a detection reagent which develops a color by specifically reacting with a detection object in a sample is placed in the detection portion (12), positional information of the detection reagent in the detection portion (12) is information on the detection object, and color development of the detection reagent can be optically read. It is preferred that a bar code is formed in the detection portion (12), and the detection reagent is placed as a part of the bar code.
Thu, 25 Aug 2016 08:00:00 EDTThe invention provides methods and compositions for the diagnosis, prognosis, and/or treatment response characterization of individuals suffering from systemic lupus erythematosus (SLE) using single cell network profiling.
Thu, 25 Aug 2016 08:00:00 EDTSome embodiments of the invention provide a system for measurement of at least two hemoglobin species in a patient's blood sample by spectroscopy, and measurement of at least pH of the blood sample by biosensor. The system comprises a disposable cartridge adapted for insertion into a slot of an analyzer, and the results are used to monitor the acid-base status of a patient. A method for monitoring the acid-base status of a patient using the system is also provided.
Thu, 25 Aug 2016 08:00:00 EDTThe present invention provides methods, compositions, kits, and devices for detecting heavy metals in dried blood (e.g., dried blood spots). For example, the present invention provides: 1) dried blood spot paper that is detectably free of heavy metals and methods of preparing such paper using organic acid; 2) dried blood extraction solutions optimized for heavy metal detection (e.g., extraction solutions containing acetic acid and/or gold); 3) methods for estimating venous blood volume from dried blood mass; and 4) kits and kit components optimized for heavy metal detection in dried blood (e.g., kits with paper detectably free of heavy metals, heavy metal free skin wipes, metal free collection case, etc.).
Thu, 25 Aug 2016 08:00:00 EDTA multi-modality sensor for physiological characterization of cells can include an array of sensing pixel groups, each sensing pixel group comprising an array of sensing pixels; a plurality of signal conditioning blocks, each signal conditioning block coupled to a corresponding one sensing pixel group of the array of sensing pixel groups to process outputs of that sensing pixel group; and a controller providing signals for independent configuration of sensing modalities for each pixel of each sensing pixel group. The pixels of the multi-modality sensor support at least two sensing modalities by including an op amp that can be shared by at least two sensing circuits and including a photodiode. A cellular culture can be applied to the multi-modality sensor and a biological measurement can be performed on the cellular culture using at least two sensing modalities of the multi-modality sensor.
Thu, 25 Aug 2016 08:00:00 EDTAn apparatus for measuring through optical means temporally resolved, optical properties, and/or phenotypes, linked to cellular homeostasis. Those temporal measurements enable the detection of cell regulation through various channels linked to homeostasis, in order to assess cell viability or early cell death through rapid diagnostic.
Thu, 25 Aug 2016 08:00:00 EDTTo a biomolecule measuring apparatus, a semiconductor sensor for detecting ions generated by a reaction between a biomolecular sample and a reagent is set. The semiconductor sensor has a plurality of cells which are arranged on a semiconductor substrate, and each of which detects ions, and a plurality of readout wires. Each of the plurality of cells has an ISFET which has a floating gate and which detects ions, a first MOSFET M2 for amplifying an output from the ISFET, and a second MOSFET M3 which selectively transmits an output from the first MOSFET to a corresponding readout wire R1. Each of the plurality of cells is provided with a third MOSFET M1 which generates hot electrons in the ISFET and which injects a charge to the floating gate of the ISFET. Here, the second MOSFET and the third MOSFET are separately controlled.
Thu, 25 Aug 2016 08:00:00 EDTA method and an instrument for determining a nutritional state of a plant with respect to one or more nutrients is provided. The method comprises the steps of recording a time series of a fluorescence induction signal of a tissue sample of the plant using a fluorometer device to obtain signal data, wherein the time series at least comprises signal data within the rising portion of the fluorescence induction signal, and determining the nutritional state from an empirical model applied to the signal data, wherein the empirical model is based on pre-recorded reference data and relates nutritional states to shape-related features in the progression of the fluorescence induction signal.
Thu, 25 Aug 2016 08:00:00 EDTA system for preparing a sample for study in a charged-particle microscope by: Providing a substantially planar sample holder having opposed faces substantially parallel to one another, comprising at least one aperture that connects said faces and across which a membrane has been mounted, which membrane comprises at least one perforation;Spanning a film of aqueous liquid across said perforation, which liquid comprises at least one study specimen suspended therein;Prior to said spanning step, placing a blotting sheet of blotting material in intimate contact with a first surface of said membrane, at a side distal from said sample holder;Depositing said aqueous liquid through said aperture and onto a second surface of said membrane, opposite said first surface; andSubsequently removing said blotting sheet from said membrane.
Thu, 25 Aug 2016 08:00:00 EDTMethods for dispensing a fluid sample on a substrate include obtaining an image of a sample applicator in proximity to the substrate, where the image includes a first image of the sample applicator and a second image of the sample applicator, determining a height of the sample applicator relative to a surface plane of the substrate based on a distance between common portions of the first and second images, and dispensing the fluid sample onto the substrate using the sample applicator, where the dispensing includes: translating the sample applicator, translating the substrate, or translating both the sample applicator and the substrate to effect a relative translation between the sample applicator and the substrate; and maintaining the sample applicator within 2 microns of a target height relative to the surface plane of the substrate during the translating.
Thu, 25 Aug 2016 08:00:00 EDTThe present invention relates to a method of producing thin sections by means of a microtome, wherein a hand wheel (32) is manually driven for producing a first thin section, and wherein the rotational movement of the hand wheel (32) is detected by means of an encoder (38), and a profile of the rotational movement is determined. The determined profile is stored and selected for production of at least a second thin section. A motor (24) is driven for generating a cutting movement between a cutting unit (16) and a sample holder (12) in accordance with the respective stored profile selected for producing the second thin section. The invention further relates to a microtome (10) by means of which the above method is carried out.
Thu, 25 Aug 2016 08:00:00 EDTThe present invention provides methods for mineral precipitation of porous particulate starting materials using isolated urease.
Thu, 25 Aug 2016 08:00:00 EDTThe present invention relates to a method of preparing a strain of sugar fermenting Saccharomyces cerevisiae with capability to ferment xylose, wherein said method comprises different procedural steps. The method comprises mating a first sporulated Saccharomyces cerevisiae strain with a second Saccharomyces cerevisiae haploid strain. Thereafter, screening for mated cells is performed, growing such mated cells, and verifying that mated cells exhibit basic morphology by microscopic inspection. Thereafter, creation of a mixture of the mated cells is performed, subjecting the mixture to continuous chemostat cultivation and obtaining the sugar fermenting Saccharomyces cerevisiae cells with capability to ferment xylose is performed. The invention also comprises strains obtained by said method.
Thu, 25 Aug 2016 08:00:00 EDTA method for measuring human CYP3A inducibility upon administration of a test drug, characterized in that a non-human animal to which a test drug is administered or a population of human cells cultured in a medium containing a test drug is infected with viruses (A) and (B); virus (A) being an adenovirus which is used as a vector and engineered by incorporating thereto a detectable reporter gene and at least 3 human PXR binding regions falling within an untranslated region of a human CYP3A gene, and virus (B) being an adenovirus which is used as a vector and engineered by incorporating thereto a human PXR cDNA; and subsequently expression level of the reporter gene is determined in the non-human animal or the cultured human cells. The present invention ensures convenient and accurate evaluation of human CYP3A inducibility upon administration of a test drug to a human subject, providing accurate evaluation in terms of the efficacy of the test drug, occurrence of side effects, disappearance of the drug effect, etc.
Thu, 25 Aug 2016 08:00:00 EDTAn improved method and kit of determining whether a patient is predisposed to having severe periodontal disease and/or having high risk of progression of periodontal disease, comprising the steps of (i) taking a biological sample from said patient; (ii) genotyping said biological sample for genetic polymorphism pattern comprising IL 1B (rs16944), IL 1B (rs1143623) and IL 1B (rs4848306); and (iii) comparing said genetic polymorphism patterns to a reference composite genotype pattern; wherein the similarity of said genetic polymorphism patterns to said reference pattern indicate said patient's predisposition to having severe periodontal disease and/or having high risk of progression of periodontal disease.
Thu, 25 Aug 2016 08:00:00 EDTThis invention refers to the methods for predicting the progress of nephritis and lupus nephritis in an individual. This invention also refers to the methods for evaluating the development of nephritis, particularly lupus nephritis, in an individual, and his/her response to a treatment.
Thu, 25 Aug 2016 08:00:00 EDTThe present invention relates to a method for real-time monitoring and/or quantification of newly-synthesized complementary deoxyribonucleic acid (cDNA) during a reverse transcription reaction of an ribonucleic acid (RNA) template in a sample, the method using a fluorogenic dye binding to RNA:cDNA hybrids. The present invention also relates to the use of this method as well as to kits employing the fluorogenic dye.
Thu, 25 Aug 2016 08:00:00 EDTSNP specific hydrolysis probe including a hairpin structure toward the 3′ end, along with kits are provided that are designed for the detection of a SNP in a target nucleic acid
Thu, 25 Aug 2016 08:00:00 EDTThe present invention relates to nucleic acid amplification assays for the detection of nucleic acid sequences of Gardnerella vaginalis. The present invention provides oligonucleotides that are complementary or that anneal to nucleic acid sequences of the vly gene of GV. The present invention also provides internal amplification controls (IACs) that can be used in nucleic acid amplification reactions.
Thu, 25 Aug 2016 08:00:00 EDTA primer set useful for further improvement of sensitivity in detection or identification of bacterial species in a sample by a PCR method, a kit for PCR using the primer set and a method of detection or identification of bacterial species in a sample using the primer set. Sensitivity in detection or identification of bacterial species in a sample by conducting a PCR method using the primer set with a minimized contamination amount of bacterial nucleic acid is improved. The primer set includes at least one primer pair in seven primer pairs obtained by selecting one primer pair from each of Groups S1 to S7 consisting of specific primers. In addition, the kit for detection of bacterial species includes at least one primer pair in seven primer pairs obtained by selecting one primer pair from each of Groups K1 to K7 consisting of specific primers.
Thu, 25 Aug 2016 08:00:00 EDTA method for steadying a thermal convection flow field in a PCR reaction solution during a thermal convective polymerase chain reaction (PCR) includes steps as follows. A PCR tube is provided. A PCR reaction solution is filled in the PCR tube. A bottom of the PCR tube is heated so that a thermal convection is induced. And the PCR to be is tilted at a tilt angle relative to a vertical line over a ground for causing a single-loop flow in the thermal convection flow field in the PCR reaction solution, whereby a denaturation an annealing reaction and an extension reaction occur sequentially and repeatedly in different temperature regions of the PCR reaction solution.
Thu, 25 Aug 2016 08:00:00 EDTAn elution container is a container that stores a liquid by sealing a first opening. The elution container includes: a first annular wall section having an annular wall surface formed around the first opening; and a first annular attachment surface which is formed on the inner side of the first annular wall section and to which a first film sealing the first opening is attached. The first annular wall section has a height higher than the first attachment surface. In addition, the elution container includes: a second annular wall section having an annular wall surface on a second end portion of the elution container; and a second annular attachment surface which is formed on the inner side of the second annular wall section and to which a second film sealing a second opening is attached. The second annular wall section has a height higher than the second attachment surface.
Thu, 25 Aug 2016 08:00:00 EDTA chip for biological and/or biochemical analyses includes: a supporting body, including a substrate of semiconductor material and a structural layer of biocompatible material; and a plurality of wells, designed to contain a liquid solution for the biological and/or biochemical analyses. Each well is formed by: a bottom chamber, defining a first containment volume for containing the liquid solution, and a top chamber, extending at least partially vertically aligned to, and in fluid communication with, the bottom chamber and defining a second containment volume greater than the first containment volume. The bottom chamber is formed, at least in part, in the biocompatible structural layer.
Thu, 25 Aug 2016 08:00:00 EDTThe present invention is directed to a diagnostic composition for use in the viscoelastic analysis of a test liquid, and to a container (1) comprising same. The composition comprises at least an activator of coagulation, and at least one further constituent selected from CaCl2 and from one or more inhibitors and/or coagulation components, wherein the composition is present in essentially dry form of all constituents and in an amount sufficient for performing one single viscoelastic analysis of a specified blood or plasma sample and wherein the constituents are not present in a substance mixture, but in a spatially separated form. The present invention is further directed to a method of performing a viscoelastic analysis on a test liquid, and to the use of the diagnostic composition in such a method.
Thu, 25 Aug 2016 08:00:00 EDTA method for assaying cellulase activity, including: a process (A) of preparing two or more substrate solutions, which have an identical absorbance measured at an identical wavelength and in which cellulose is dispersed at an identical concentration, and measuring the absorbance of each of the substrate solutions; a process (B) of respectively adding different kinds of enzyme solutions to the substrate solutions, and performing an enzyme reaction under the same conditions; a process (C) of measuring the absorbance of each of the substrate solutions after the enzyme reaction; a process (D) of calculating the absorbance decrease values of the substrate solutions before and after the enzyme reaction; and a process (E) of assaying cellulase activities of the enzyme solutions based on the absorbance decrease values. In the assay of the process (E), it is determined that the enzyme solutions have higher cellulase activity as the absorbance decrease values become larger.
Thu, 25 Aug 2016 08:00:00 EDTProvided is a diagnostic apparatus. The diagnostic apparatus includes a microfluidic chip including first and second measurement parts for respectively measuring an amount of hemoglobin and an active degree of an enzyme within a blood sample. The second measurement part of the microfluidic chip analyzes the active degree of the enzyme within the blood sample using voltammetry.
Thu, 25 Aug 2016 08:00:00 EDTA medium for the culture and detection of target microorganisms, having at least one natural or synthetic specific substrate configured to detect at least one enzyme activity or metabolic activity of the target microorganisms and at least one compound that inhibits or delays the germination of spores of microorganisms, other than the target microorganisms, that are capable of interfering with the culture and detection of the target microorganisms.
Thu, 25 Aug 2016 08:00:00 EDTThe present disclosure relates to methods and devices for providing accurate measurement of a property of a sample. The method comprises obtaining a plurality of independent measurements of the property. The plurality of values of the property of the sample obtained by the plurality of independent measurements is compared to determine whether one or more of the values is an outlier.
Thu, 25 Aug 2016 08:00:00 EDTAn expression system for expressing a protein comprising: a eukaryotic host cell carrying a dihydrofolate reductase (DHFR) deficiency; and an expression vector, the expression vector encoding the human growth hormone gene; a expression vector, the expression vector comprising: a eukaryotic selectable marker including a minimal SV 40 early promoter driving expression of a sequence encoding dihydrofolate reductase for complementing the DHFR deficiency in the host cell; a prokaryotic selectable marker conveying Ampicillin resistance to a prokaryotic host cell; a prokaryotic Origin of Replication; a plurality of multiple cloning sites (MCS); and at least one protein expression module comprising: a Simian Vacuolating Virus 40 (SV40) early promoter, inclusive of its 72 bp enhancer repeats; and a rabbit β-globin intron sequence being separable from a SV40 p A sequence by a first multiple cloning site, for receiving a coding sequence and expressing a desired protein therefrom.
Thu, 25 Aug 2016 08:00:00 EDTA method for recombinantly expressing a macromolecule in a host cell is disclosed which involves culturing a host cell which contains two nucleic acid sequences, i.e., a first nucleic acid sequence encoding a membrane-permeabilizing agent and a second nucleic acid sequence encoding a desired macromolecule under the operative control of an inducible promoter, to a selected cell density that permits accumulation of the agent. Thereafter the host cell is exposed to an environmental condition that induces the agent to disrupt the integrity of the cell membrane without complete lysis of the cell membrane. The host cell thereby allows transport through the membrane of small molecular weight compounds. These resulting host cells are cultured in the presence of a nutrient cocktail that contains components that can transport through the disrupted cell membrane, e.g., an inducing agent that induces the tightly regulated promoter and metabolic requirements that permit expression of the macromolecule. Alternatively, a method for enhancing recombinant expression of a macromolecule in a host cell comprises contacting a host cell at a suitable cell density with a membrane-permeabilizing agent that disrupts the integrity of the cell membrane without complete lysis of the membrane, and allows transport through the membrane of small molecular weight compounds. The host cell contains a nucleic acid sequence encoding a macromolecule under the operative control of an inducible promoter. These cells are then cultured in the presence of an above-described nutrient cocktail, and permits enhanced expression of the macromolecule in the membrane-disrupted host cell. Each method can also be employed in methods for in situ drug screening, among other uses.
Thu, 25 Aug 2016 08:00:00 EDTBiomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce an intermediate or product, e.g., by fermentation.
Thu, 25 Aug 2016 08:00:00 EDTA method for producing saccharides according to the present invention includes a step of preparing a slurry by adding an aqueous solution containing a sugar or a sugar solution to biomass so that the slurry has a biomass content of 10 to 30 w/v %, and a step of adding at least one of an enzyme that degrades cellulose and an enzyme that degrades hemicellulose to the slurry containing the biomass to degrade at least one of cellulose and hemicellulose contained in the biomass, thereby producing saccharides containing glucose as a main component.